In vitro propagation of cassava (Manihot esculenta Crantz)

1986 ◽  
Vol 6 (3) ◽  
pp. 221-228 ◽  
Author(s):  
Michael K. Smith ◽  
Brenda J. Biggs ◽  
Kenneth J. Scott
Keyword(s):  
Manihot Esculenta ◽  
In Vitro Propagation ◽  
Manihot Esculenta Crantz

scholarly journals In vitro propagation of Manihot esculenta Crantz in alternative substrate: Ammonium nitrate, potassium bi phosphate, Zea mays extracts and soil

2020 ◽  
Vol 8 (10) ◽  
pp. 237-244
Author(s):  
Duru Maduabuchi ◽  
◽  
Mbata Ikechukwu ◽  
Osikwe Keziah ◽  
Ukaoma Adamma ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Zea Mays ◽  
Somatic Cell ◽  
Ammonium Nitrate ◽  
Shoot Regeneration ◽  
Manihot Esculenta ◽  
In Vitro Propagation ◽  
Gelling Agent ◽  
Manihot Esculenta Crantz

The study investigated an in vitro propagation of Manihot esculenta Crantz in a substituted substrate regime. The aim was to proffer and affordable alternative to the expensive high tech media formulations usually employed in tissue culture protocol. The experiment was conducted on laboratory bench, using standard tissue culture and micropropagation methods under aseptic conditions. The morphogenesis effect of the substrate was determined based on the integer number of explants’ callus and adventitious shoot regeneration. Results showed that MS + Agar, supported embryogenic callus formation with 38% viability, NH4NO3 + KH2PO4 + Agar, supported same with 29%. MS + 2, 4-D + BAP +Agar supported shoot establishment with 32%. While NH4NO3 + KH2PO4 + Zea mays extracts + Agar, did same with 43.26%. MS + Soil, supported callugenesis with 27% viability while NH4NO3 + KH2PO4 + Soil supported the callus establishment with 25%. MS + 2,4 - D + BAP + Soil, supported shoot establishment with 38.41% viability while NH4NO3 + KH2PO4 + Zea mays Extracts + Soil supported same with 36%. The application of crude Zea mays seedling extracts can serve as potent alternative to the synthetic 2, 4 – D and BAP, in in vitro somatic cell morphogenesis. NH4NO3 + KH2 + PO4 can substitute for the MS salt in the same protocol. Loamy top soil can be a good alternative to agar powder as gelling agent in cassava somatic cell embryogenesis and shoot regeneration. Keywords: Ammonium nitrate, Potassium biphosphate, MS salt, axillary meristem, morphogenesis.

scholarly journals Development of in vitro propagation protocol for some recalcitrant cassava (Manihot esculenta Crantz) genotypes in Sierra Leone

2018 ◽  
Vol 17 (18) ◽  
pp. 606-613 ◽  
Author(s):  
Veronica Sesay Janatu ◽  
Gado G. Yamba Nicole ◽  
Sherman-Kamara Joseeph ◽  
David Quee Dan
Keyword(s):  
Sierra Leone ◽  
Manihot Esculenta ◽  
In Vitro Propagation ◽  
Manihot Esculenta Crantz

scholarly journals Optimization of In-Vitro Propagation of Cassava (Manihot esculenta Crantz) Genotypes

2013 ◽  
Vol 5 (3) ◽  
Author(s):  
Mapayi E. F. ◽  
Ojo D. K. ◽  
Oduwaye O. A. ◽  
Porbeni J. B. O.
Keyword(s):  
Manihot Esculenta ◽  
In Vitro Propagation ◽  
Manihot Esculenta Crantz

scholarly journals In vitro embryo rescue and plant regeneration following self-pollination with irradiated pollen in cassava (Manihot esculenta Crantz)

2015 ◽  
Vol 14 (27) ◽  
pp. 2191-2201
Author(s):  
Buttibwa Mary ◽  
S Kawuki Robert ◽  
K Tugume Arthur ◽  
Akol Jacinta ◽  
Magambo Stephen ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Manihot Esculenta ◽  
Embryo Rescue ◽  
Manihot Esculenta Crantz ◽  
Irradiated Pollen ◽  
Self Pollination

scholarly journals In vitro meristem-tip culture and regeneration approaches in Congolese cassava accessions (Manihot esculenta Crantz cv. Boma and cv. Mpelo-Nlongi)

2015 ◽  
Vol 3 (2) ◽  
pp. 72
Author(s):  
J.-Roger Bansimba Mukiese ◽  
Aimé Diamuini Ndofunsu ◽  
Freddy Bulubulu ◽  
Alexandre Mbaya Ntumbula ◽  
Sébastien Luyindula Ndiku
Keyword(s):  
Growth Regulators ◽  
Manihot Esculenta ◽  
Callus Formation ◽  
Shoot Development ◽  
Media Composition ◽  
Manihot Esculenta Crantz ◽  
Adenine Sulphate ◽  
Meristem Tip Culture ◽  
The Media

<p>Shiny dome-like structures measuring less than 1mm in length were excised aseptically from shoot tip buds of infected of two cassava (<em>Manihot esculenta</em> Crantz) local cultivars (Boma and Mpelo Nlongi) and cultivated <em>in vitro</em> in two types of media with different combination of growth hormone: Murashige and Skoog supplemented of sucrose (20 g/l), Naphtalenacetic acid (NAA, 10 μM), Ben-zylaminopurine (BAP, 0.66 μM) as well as Gibberellic acid (GA3, 0.1 μM) with 80 mg/l of Adenine sulphate and MS-free growth regulators. After four weeks, data were scored: 29.5% responding explant with callus formation and 20.5% responding explants to shoot development in the medium with growth regulators for the cultivar Boma whereas the cultivar Mpelo-Nlongi presented 5.7% and 25.7% respectively of callus formation and shoot development. The cultivar Boma presented a tendency more pronounced for the callus formation rather than with the shoot development contrary to the cultivar Mpelo-Nlongi. In regards of this experiment, it was shown that the media composition and genotype are essential factors, which influence in vitro growth, mainly the shoot development, in the culture of meristems for cassava local accessions.</p>

scholarly journals In vitro assessment of the genotoxic and cytotoxic effects of boiled juice (tucupi) from Manihot esculenta Crantz roots

2016 ◽  
Vol 15 (4) ◽  
Author(s):  
L.A. Cunha ◽  
T.C. Mota ◽  
P.C.S. Cardoso ◽  
D.D.F.A. Alcântara ◽  
R.M.R. Burbano ◽  
...  
Keyword(s):  
Manihot Esculenta ◽  
Manihot Esculenta Crantz ◽  
Cytotoxic Effects ◽  
In Vitro Assessment

Efeitos de diferentes concentrações de 6-Benzilaminopurina no cultivo in vitro de Manihot esculenta Crantz

2015 ◽  
Vol 4 (1) ◽  
pp. 105
Author(s):  
Simone da Silva ◽  
Flávio Freires Ferreira ◽  
Arlena Maria Guimarães Gato
Keyword(s):  
Manihot Esculenta ◽  
Manihot Esculenta Crantz
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