scholarly journals In vitro meristem-tip culture and regeneration approaches in Congolese cassava accessions (Manihot esculenta Crantz cv. Boma and cv. Mpelo-Nlongi)

2015 ◽  
Vol 3 (2) ◽  
pp. 72
Author(s):  
J.-Roger Bansimba Mukiese ◽  
Aimé Diamuini Ndofunsu ◽  
Freddy Bulubulu ◽  
Alexandre Mbaya Ntumbula ◽  
Sébastien Luyindula Ndiku
Keyword(s):  
Growth Regulators ◽  
Manihot Esculenta ◽  
Callus Formation ◽  
Shoot Development ◽  
Media Composition ◽  
Manihot Esculenta Crantz ◽  
Adenine Sulphate ◽  
Meristem Tip Culture ◽  
The Media

<p>Shiny dome-like structures measuring less than 1mm in length were excised aseptically from shoot tip buds of infected of two cassava (<em>Manihot esculenta</em> Crantz) local cultivars (Boma and Mpelo Nlongi) and cultivated <em>in vitro</em> in two types of media with different combination of growth hormone: Murashige and Skoog supplemented of sucrose (20 g/l), Naphtalenacetic acid (NAA, 10 μM), Ben-zylaminopurine (BAP, 0.66 μM) as well as Gibberellic acid (GA3, 0.1 μM) with 80 mg/l of Adenine sulphate and MS-free growth regulators. After four weeks, data were scored: 29.5% responding explant with callus formation and 20.5% responding explants to shoot development in the medium with growth regulators for the cultivar Boma whereas the cultivar Mpelo-Nlongi presented 5.7% and 25.7% respectively of callus formation and shoot development. The cultivar Boma presented a tendency more pronounced for the callus formation rather than with the shoot development contrary to the cultivar Mpelo-Nlongi. In regards of this experiment, it was shown that the media composition and genotype are essential factors, which influence in vitro growth, mainly the shoot development, in the culture of meristems for cassava local accessions.</p>

scholarly journals Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz)

2018 ◽  
Vol 21 (2) ◽  
pp. 45
Author(s):  
Idha Susanti ◽  
Suharsono Suharsono ◽  
Utut Widyastuti ◽  
Ulfah Juniarti Siregar ◽  
Aris Tjahjoleksono
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Manihot Esculenta ◽  
Callus Formation ◽  
Induction Treatment ◽  
Manihot Esculenta Crantz ◽  
Friable Callus ◽  
Apical Bud ◽  
Different Response

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media.  This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment.  Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and  half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”.  All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos

Plant regeneration from stem callus of cassava

1979 ◽  
Vol 57 (16) ◽  
pp. 1761-1763 ◽  
Author(s):  
J. P. Tilquin
Keyword(s):  
Plant Regeneration ◽  
Gibberellic Acid ◽  
Shoot Apical Meristem ◽  
Manihot Esculenta ◽  
Apical Meristem ◽  
Callus Formation ◽  
Shoot Development ◽  
Meristem Culture ◽  
Manihot Esculenta Crantz ◽  
Leaf Differentiation

Callus formation and organogenesis have been induced on the internode culture of cassava (Manihot esculenta Crantz). Callusing was rapidly induced on the medium devised by Kartha, Gamborg, and Constabel for the shoot apical meristem culture of cassava. During culture, green protuberances appear on the callus followed by the differentiation of a leaf-like structure. The leaf-like structure degenerated after a month of differentiation. On the same medium, but lacking gibberellic acid (GA3), organogenesis is less frequent and precocious but the leaves which appear are typical of cassava; leaf differentiation is followed by shoot development.

scholarly journals The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro

2021 ◽  
Vol 20 (5) ◽  
pp. 127-138
Author(s):  
Danuta Kozak ◽  
Marzena Parzymies ◽  
Alicja Świstowska ◽  
Barbara Marcinek ◽  
Elżbieta Pogroszewska
Keyword(s):  
Growth Regulators ◽  
Growth And Development ◽  
Callus Formation ◽  
Propagation Rate ◽  
Multiplication Rate ◽  
Explant Position ◽  
Murashige And Skoog Medium ◽  
The Media ◽  
Pot Plant

Mandevilla is a valuable ornamental pot vine. However, due to a low propagation rate, it is difficult to keep up with the demand. Micropropagation would allow to produce lots of plants for the market. The aim of the study was to determine the effect of the growth regulators addition to the media and explants orientation on multiplication of Mandevilla sanderi, an exotic, ornamental pot plant. The shoot tips were placed vertically or horizontally on the Murashige and Skoog medium supplemented with benzyladenine (BA) or isopentenyladenine (2iP), at concentrations of 1, 2.5 or 5 mg·dm–3 singly or in combination with thidiazuron (TDZ) at concentrations of 0.01, 0.025 or 0.05 mg·dm–3. Maximum multiplication rate was noted on the media supplemented with 2.5 mg·dm–3 2iP + 0.025 mg·dm–3 TDZ or 5 mg·dm–3 2iP, when explants were placed horizontally. All the treatments resulted in callus formation. Medium supplemented with the highest concentration of BA combined with TDZ was the most active in callus growth.

scholarly journals Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz)

2017 ◽  
Vol 21 (2) ◽  
pp. 45
Author(s):  
Idha Susanti ◽  
Suharsono Suharsono ◽  
Utut Widyastuti ◽  
Ulfah Juniarti Siregar ◽  
Aris Tjahjoleksono
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Manihot Esculenta ◽  
Callus Formation ◽  
Induction Treatment ◽  
Manihot Esculenta Crantz ◽  
Friable Callus ◽  
Apical Bud ◽  
Different Response

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media.  This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment.  Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and  half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”.  All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos

scholarly journals In vitro embryo rescue and plant regeneration following self-pollination with irradiated pollen in cassava (Manihot esculenta Crantz)

2015 ◽  
Vol 14 (27) ◽  
pp. 2191-2201
Author(s):  
Buttibwa Mary ◽  
S Kawuki Robert ◽  
K Tugume Arthur ◽  
Akol Jacinta ◽  
Magambo Stephen ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Manihot Esculenta ◽  
Embryo Rescue ◽  
Manihot Esculenta Crantz ◽  
Irradiated Pollen ◽  
Self Pollination

scholarly journals In vitro shoot regeneration through anther culture of Brassica spp.

1970 ◽  
Vol 35 (2) ◽  
pp. 331-341 ◽  
Author(s):  
MA Sayem ◽  
M Maniruzzaman ◽  
SS Siddique ◽  
M Al-Amin
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Maximum Rate ◽  
Media Composition ◽  
Shoot Initiation ◽  
The Media

The experiment was conducted to investigate the performance of three different genotypes (BARI Sarisha-6, BARI Sarisha-8, and BARI Sarisha-11) in two different media viz., MS and B5 with different concentrations of phytohormone (2, 4-D) for callus induction from uninucleate stage anthers of Brassica and subsequent plant regeneration in MS media with different concentrations of phytohormone (BAP and NAA). Among the genotypes, BARI Sarisha-8 showed the best performance for all the parameters of callus induction. The performance of BARI Sarisha-6 was poor compared to others. Maximum rate of callus induction (%) was observed in MS + 0.5 mg/L 2, 4-D followed by B5 + 0.5 mg/L 2,4-D. The media combination MS + 1.0 mg/L BAP 0.3 mg/L 2,4-D showed the best performance for maintenance of calli. Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1. The genotype BARI Sarisha-8 produced higher percent of shoots/calli and required minimum days for shoot initiation. Higher percent calli without shoot were produced by the genotype BARI Sarisha-6. The media combination MS + 2.0 mg/L BAP + 0.5 mg/L NAA showed the best performance for shoot regeneration and required maximum days for shoot initiation. Keywords: Regeneration; BARI Sarisha-6; BARI Sarisha-8; BARI Sarisha-11; anther culture; phytohormone  DOI: 10.3329/bjar.v35i2.5896Bangladesh J. Agril. Res. 35(2) : 331-341, June 2010

Organogenesis and chromosome number in callus derived from cassava anthers

1978 ◽  
Vol 56 (10) ◽  
pp. 1287-1290 ◽  
Author(s):  
Ming-Chin Liu ◽  
Wen-Huei Chen
Keyword(s):  
Manihot Esculenta ◽  
Callus Formation ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Chromosome Counts ◽  
Manihot Esculenta Crantz ◽  
Somatic Tissues ◽  
Mitotic Figures ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Chlorophyll Formation

Experiments have been performed to induce callus formation and organogenesis in anther culture of cassava (Manihot esculenta Crantz). Callusing was achieved on a modified Murashige and Skoog medium (MSB) supplemented with 4.44 μM 6-benzylaminopurine (BAP) and 4.52 μM 2,4-dichlorophenoxyacetic acid (2.4-D). No callus was formed from anthers pretreated at 4 °C for more than 48 h or on a medium containing 4g/ℓ activated charcoal. Callus on MSB with 4.44–8.88 μM BAP alone formed roots only. BAP (8.88 μM) in combination with α-naphthalene acetic acid (NAA) (10.74 μM) resulted in chlorophyll formation in callus. Abscisic acid (ABA) acted as an antagonist to NAA in reducing the frequency of callus greening when the latter was applied jointly with BAP. Chromosome counts of mitotic figures from callus cells ranged from 34 to 38 indicating that the calli were derived from the somatic tissues of the anthers.

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