In vitro Propagation of Malaysian Cassava (Manihot esculenta Crantz) Variety through Low Cost Tissue Culture Media

The study investigated an in vitro propagation of Manihot esculenta Crantz in a substituted substrate regime. The aim was to proffer and affordable alternative to the expensive high tech media formulations usually employed in tissue culture protocol. The experiment was conducted on laboratory bench, using standard tissue culture and micropropagation methods under aseptic conditions. The morphogenesis effect of the substrate was determined based on the integer number of explants’ callus and adventitious shoot regeneration. Results showed that MS + Agar, supported embryogenic callus formation with 38% viability, NH4NO3 + KH2PO4 + Agar, supported same with 29%. MS + 2, 4-D + BAP +Agar supported shoot establishment with 32%. While NH4NO3 + KH2PO4 + Zea mays extracts + Agar, did same with 43.26%. MS + Soil, supported callugenesis with 27% viability while NH4NO3 + KH2PO4 + Soil supported the callus establishment with 25%. MS + 2,4 - D + BAP + Soil, supported shoot establishment with 38.41% viability while NH4NO3 + KH2PO4 + Zea mays Extracts + Soil supported same with 36%. The application of crude Zea mays seedling extracts can serve as potent alternative to the synthetic 2, 4 – D and BAP, in in vitro somatic cell morphogenesis. NH4NO3 + KH2 + PO4 can substitute for the MS salt in the same protocol. Loamy top soil can be a good alternative to agar powder as gelling agent in cassava somatic cell embryogenesis and shoot regeneration. Keywords: Ammonium nitrate, Potassium biphosphate, MS salt, axillary meristem, morphogenesis.

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Effectiveness of tissue culture media components on the growth and development of cauliflower (Brassica oleracea var. Botrytis) seedling explants in vitro

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb12.1984 ◽

2012 ◽

Vol 11 (76) ◽

Author(s):

Ehab M. R.

Keyword(s):

Tissue Culture ◽

Brassica Oleracea ◽

Growth And Development ◽

Culture Media ◽

Tissue Culture Media ◽

Seedling Explants

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In vitro tissue culture and genetic analysis of two high-CBD medical cannabis (Cannabis sativa L.) breeding lines

Genetika ◽

10.2298/gensr2003925m ◽

2020 ◽

Vol 52 (3) ◽

pp. 925-941 ◽

Cited By ~ 1

Author(s):

Spela Mestinsek-Mubi ◽

Sinja Svetik ◽

Marko Flajsman ◽

Jana Murovec

Keyword(s):

Tissue Culture ◽

Cannabis Sativa ◽

Therapeutic Potential ◽

Culture Media ◽

Shoot Culture ◽

Medical Cannabis ◽

Breeding Lines ◽

In Vitro Tissue Culture ◽

Tissue Culture Media

The species Cannabis sativa L. has recently witnessed a resurgence of interest all over the world due to its multipurpose applications and the scientific confirmation of its pharmacological properties. Genotypes with high cannabinoid content are appreciated in the pharmaceutical and cosmetic industries due to their therapeutic potential. These genotypes, with predominantly high cannabidiol (CBD) content, are the subject of research and breeding in several programs, but to date, little data is published on the in vitro tissue culture of cannabis. Our study focused on the establishment of an efficient micropropagation method for two high-CBD breeding lines (MX-CBD-11 and MX-CBD-707) as the basis for advanced biotechnological breeding approaches. The results demonstrated that the in vitro culture of medical cannabis can be initiated on different culture media, that cultured plants can be successfully acclimatized, and that nodal position, and especially the genotype, have a significant influence on the success of shoot culture establishment. They showed that the published tissue culture media optimized for one high-THC strain of Mexican cannabis are not as efficient for other genotypes of (medical) cannabis. We complemented this research with a genetic study of 95 plants of the two breeding lines with 16 microsatellite (SSR) markers which clustered the plants based on breeding line. The results demonstrated that only 8 markers are needed for discrimination of all analyzed plants and their usefulness for clonal identification.

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Low-Cost Alternatives for Conventional Tissue Culture Media

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2018.704.288 ◽

2018 ◽

Vol 7 (04) ◽

pp. 2523-2529 ◽

Cited By ~ 1

Author(s):

Dipak D. Kadam ◽

Amit A. Chhatre ◽

Shivaji A. Lavale ◽

Nalini A. Shinde

Keyword(s):

Tissue Culture ◽

Culture Media ◽

Low Cost ◽

Tissue Culture Media

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Low Cost Tissue Culture Technologies in Vegetables: A Review

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2020/v29i930226 ◽

2020 ◽

pp. 66-78

Author(s):

Reshav Naik ◽

Anil Bhushan ◽

R. K. Gupta ◽

Anamika Walia ◽

Anshika Gaur

Keyword(s):

Tissue Culture ◽

Culture Media ◽

Callus Formation ◽

Low Cost ◽

Direct Regeneration ◽

Clonal Variation ◽

In Vitro Mutagenesis ◽

Vegetable Crops ◽

Brown Sugar

The demand of vegetable crops is increasing day by day due to changes in consumption patterns, so the need of the hour is to develop technologies that enhance the vegetable production at a rapid rate. Plant Tissue culture is one such remarkable biotechnological tool that has its application in vegetable propagation and improvement, disease elimination, herbicide resistance, salinity tolerance, incorporation of high nutrient content, genetically improved plants and conservation of endangered plant species and in the near future usage of this technology is going to increase further manifold. It is used for production of disease free quality planting material and development of varieties through direct regeneration, anther/ovule culture, somatic embryogenesis etc. or for creation of new variation (organogenesis via callus formation, soma-clonal variation and in vitro mutagenesis). In spite of being a very important and viable non-conventional biotechnological tool, high cost of production of seedlings in vitro remains a major impediment in popularization of this technology. High cost of producing seedlings is due to availability of limited resources, high recurrent costs of consumables for media and lack of awareness, which limits its application only to a few institutions and rich farmers especially in developing countries. Therefore, in order to make this technology a successful and viable option for the farmers, future thrust must be on cost reduction of in vitro seedlings. The components of tissue culture technology such as culture media components, glassware, lighting and water for media preparation can be replaced with low cost alternatives to reduce the overall cost of tissue culture. The usage of alternatives for gelling agent’s like isabgol (potato, tomato, cassava, turmeric, ginger), sago (potato, tomato, turmeric, ginger) cassava starch (potato, cassava, sweet potato) barley starch, phytagel etc. and for carbon sources like table sugar (potato, turmeric, ginger), jaggery, sugarcane juice, cube sugar (bittergord), brown sugar etc have already been documented worldwide. The present paper reviews the work done by researchers around the globe in developing various low cost alternative technologies with focus on vegetable crops.

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Cultivation of Clinically Significant Hemoflagellates

Clinical Microbiology Reviews ◽

10.1128/cmr.15.3.374-389.2002 ◽

2002 ◽

Vol 15 (3) ◽

pp. 374-389 ◽

Cited By ~ 53

Author(s):

Frederick L. Schuster ◽

James J. Sullivan

Keyword(s):

Tissue Culture ◽

Culture Media ◽

Life Cycles ◽

Variable Component ◽

Culture Cells ◽

Tissue Culture Media ◽

Oriental Sore ◽

Clinically Significant ◽

Cultivation In Vitro

SUMMARY The hemoflagellates, Trypanosoma spp. and Leishmania spp., are causal agents of a number of parasitic diseases having a major impact on humans and domestic animals over vast areas of the globe. Among the diseases are some of the most pernicious and deadly of human afflictions: African sleeping sickness, Chagas' disease, kala-azar, and Oriental sore. The organisms have complex, pleomorphic life cycles typically involving a vertebrate and an invertebrate host, the latter serving as a vector. In the vertebrate host, they are primarily blood and tissue parasites. In their transition from one host to another, the hemoflagellates undergo morphological, physiological, and biochemical changes that facilitate their growth and subsequent transmission. A major goal in the study of the hemoflagellates has been the cultivation in vitro of both vertebrate and invertebrate stages of the organisms. The first types of media used in their cultivation, and still useful for establishment of cultures, were undefined and contained a complex of ingredients. These gave way to semidefined formulations which included tissue culture media as a base and, as a next step, addition of tissue culture cells as a feeder layer to promote parasite growth. More recently developed media are completely defined, having replaced the feeder cells with various supplements. Serum, a sometimes-variable component of the media, can be replaced by various serum substitutes. This review focuses on the hemoflagellates that infect humans, describing stages in the development of media leading to the fully defined formulations that are now available for the cultivation of many of these organisms.

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Elaboration and characterization of low cost ceramics microfiltration membranes applied to the sterilization of plant tissue culture media

Journal of the Taiwan Institute of Chemical Engineers ◽

10.1016/j.jtice.2015.07.032 ◽

2016 ◽

Vol 59 ◽

pp. 79-85 ◽

Cited By ~ 9

Author(s):

Nabila medjemem ◽

Abdelhamid Harabi ◽

Ferhat Bouzerara ◽

Lazhar Foughali ◽

Boukhemis Boudaira ◽

...

Keyword(s):

Tissue Culture ◽

Plant Tissue Culture ◽

Plant Tissue ◽

Culture Media ◽

Low Cost ◽

Tissue Culture Media ◽

Microfiltration Membranes

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Der Einfluß von Protoplastenmedien auf die Tumorinduktion an Kalanchoe-Blättern / The Influence of Protoplast Media on the Tumor Induction on Kalanchoe Leaves

Zeitschrift für Naturforschung C ◽

10.1515/znc-1975-1-216 ◽

1975 ◽

Vol 30 (1-2) ◽

pp. 73-76 ◽

Cited By ~ 2

Author(s):

Rolf Beiderbeck

Keyword(s):

Tissue Culture ◽

Agrobacterium Tumefaciens ◽

Culture Media ◽

Plant Cells ◽

Tumor Induction ◽

Tissue Culture Media ◽

Complete Knowledge ◽

Tumor Transformation ◽

Effects Of Media

Abstract Experiments concerning tumor transformation of plant cells or protoplasts need complete knowledge of the effects of media components. Here inhibiting effects of cellulase Onozuka, glucose, sucrose and mannitol (up to 0.3 M) , of tissue culture media, and of the antibiotic aureo-mycin on the tumor initiation process in Kalanchoe leaves are reported. Also the influence of these substances on the growth of the tumor inducing Agrobacterium tumefaciens is studied. The results lead to the design of conditions which should be favourable to in vitro transformation.

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