Doubled haploid sunflower (Helianthus annuus) plant production by androgenesis: fact or artifact? Part 2. In vitro isolated microspore culture

1995 ◽  
Vol 41 (3) ◽  
pp. 203-309 ◽  
Author(s):  
Marc Coumans ◽  
Danni Zhong
Keyword(s):  
Helianthus Annuus ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Plant Production ◽  
Isolated Microspore Culture

scholarly journals Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus

2020 ◽  
Author(s):  
Eduardo Berenguer ◽  
Elena A Minina ◽  
Elena Carneros ◽  
Ivett Bárány ◽  
Peter V Bozhkov ◽  
...  
Keyword(s):  
Cell Death ◽  
Brassica Napus ◽  
Embryonic Development ◽  
Doubled Haploid ◽  
Microspore Embryogenesis ◽  
Brassica Species ◽  
Cysteine Proteases ◽  
Plant Production ◽  
Haploid Plant

Abstract Microspore embryogenesis is a biotechnological process that allows us to rapidly obtain doubled-haploid plants for breeding programs. The process is initiated by the application of stress treatment, which reprograms microspores to embark on embryonic development. Typically, a part of the microspores undergoes cell death that reduces the efficiency of the process. Metacaspases (MCAs), a phylogenetically broad group of cysteine proteases, and autophagy, the major catabolic process in eukaryotes, are critical regulators of the balance between cell death and survival in various organisms. In this study, we analyzed the role of MCAs and autophagy in cell death during stress-induced microspore embryogenesis in Brassica napus. We demonstrate that this cell death is accompanied by the transcriptional upregulation of three BnMCA genes (BnMCA-Ia, BnMCA-IIa and BnMCA-IIi), an increase in MCA proteolytic activity and the activation of autophagy. Accordingly, inhibition of autophagy and MCA activity, either individually or in combination, suppressed cell death and increased the number of proembryos, indicating that both components play a pro-cell death role and account for decreased efficiency of early embryonic development. Therefore, MCAs and/or autophagy can be used as new biotechnological targets to improve in vitro embryogenesis in Brassica species and doubled-haploid plant production in crop breeding and propagation programs.

scholarly journals RETRACTED ARTICLE: Doubled haploid production in alfalfa (Medicago sativa L.) through isolated microspore culture

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dengxia Yi ◽  
Jifeng Sun ◽  
Yanbin Su ◽  
Zongyong Tong ◽  
Tiejun Zhang ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Haploid Production ◽  
Retracted Article ◽  
Medicago Sativa L

IN VITRO ANTHER AND ISOLATED MICROSPORE CULTURE AS TOOLS IN SWEET AND SPICE PEPPER BREEDING

2009 ◽  
pp. 61-64 ◽  
Author(s):  
A. Gémes Juhász ◽  
Z. Kristóf ◽  
P. Vági ◽  
C. Lantos ◽  
J. Pauk
Keyword(s):  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Pepper Breeding

scholarly journals Tradition, quality and biotechnology in Hungarian spice pepper ( Capsicum annuum L.) breeding

2010 ◽  
Vol 58 (3) ◽  
pp. 259-266 ◽  
Author(s):  
J. Pauk ◽  
C. Lantos ◽  
G. Somogyi ◽  
P. Vági ◽  
Z. Ábrahám Táborosi ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Culture Methods ◽  
Culture Techniques ◽  
New Genotype ◽  
Breeding Programmes ◽  
History Of ◽  
Dh Lines ◽  
Two Hybrid

Spice pepper production has a history of almost 300 years in the southern part of Hungary. In this study the results of two biotechnological improvements are summarized. Anther and isolated microspore culture techniques were improved to release haploid and doubled haploid (DH) lines for spice pepper breeding. Both the anther and isolated microspore culture methods were successfully used in spice pepper haploid production. Microspore culture-derived structures were analysed to identify their different parts. Green plantlets were regenerated from embryos derived from both anther and microspore cultures. Their doubled haploid analogues were integrated into Hungarian spice pepper hybrid seed breeding programmes. One hybrid, Sláger, was released as a new genotype for spice pepper production in 2008 and two hybrid candidates (Délibáb and Bolero) are now being tested in official trials.

scholarly journals Androgenesis of Red Cabbage in Isolated Microspore Culture In Vitro

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1950
Author(s):  
Anna Mineykina ◽  
Ludmila Bondareva ◽  
Alexey Soldatenko ◽  
Elena Domblides
Keyword(s):  
Culture Medium ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Antioxidant Properties ◽  
F1 Hybrids ◽  
Anthocyanin Content ◽  
Isolated Microspore Culture ◽  
Culture In Vitro ◽  
Red Cabbage

Red cabbage belongs to the economically important group of vegetable crops of the Brassicaceae family. A unique feature of this vegetable crop that distinguishes it from other members of the family is its unique biochemical composition characterized by high anthocyanin content, which gives it antioxidant properties. The production mainly uses F1 hybrids, which require constant parental lines, requiring 6–7 generations of inbreeding. Culture of isolated microspores in vitro is currently one of the promising methods for the accelerated production of pure lines with 100% homozygosity. The aim of this study is to investigate the factors and select optimal parameters for successful induction of red cabbage embryogenesis in isolated microspore culture in vitro and subsequent regeneration of DH plants. As a result of research, for the first time, it was possible to carry out the full cycle of obtaining DH plants of red cabbage from the induction of embryogenesis to their inclusion in the breeding process. The size of buds containing predominantly microspores at the late vacuolated stage and pollen at the early bi-cellular stage has to be selected individually for each genotype, because the embryoid yield will be determined by the interaction of these two factors. In the six samples studied, the maximum embryoid yield was obtained from buds 4.1–4.4 mm and 4.5–5.0 mm long, depending on the genotype. Cultivation of microspores was carried out on liquid NLN culture medium with 13% sucrose. The maximum number of embryoids (173.5 ± 7.5 pcs./Petri dish) was obtained on culture medium with pH 5.8 and heat shock at 32 °C for 48 h. Successful embryoid development and plant regeneration by direct germination from shoot apical meristem were achieved on MS culture medium with 2% sucrose and 0.7% agar, supplemented with 6-benzylaminopurine at a concentration of 1 mg/L. Analysis of the obtained regenerated plants, which successfully passed the stage of adaptation to ex vitro conditions by flow cytometry, showed that most of them were doubled haploids (up to 90.9%). A low number of seeds produced by self-fertilization in DH plants was observed.

scholarly journals Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses

2019 ◽  
Vol 55 (No. 3) ◽  
pp. 101-109 ◽  
Author(s):  
Olfa Slama-Ayed ◽  
Imen Bouhaouel ◽  
Sourour Ayed ◽  
Jacques De Buyser ◽  
Emmanuel Picard ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Triticum Turgidum ◽  
Microspore Culture ◽  
Plant Production ◽  
Haploid Plant ◽  
Interspecific Crosses ◽  
Isolated Microspore Culture ◽  
Haploid Production ◽  
Good Ability ◽  
Successful Approach

This study presents the first report comparing the efficiency of microspore culture, gynogenesis and durum wheat × maize crosses for haploid plant production from three durum wheat genotypes (Razzek, Karim and Jneh Khotifa). The results showed that the best induction, calli or embryos formation and plant regeneration rates for the three genotypes were obtained with gynogenesis (47.2, 7.6, 0.8%), followed by interspecific crosses (33.1, 1.7, 0.4%) and isolated microspore culture (8.2, 0.05, 0.01%). Interestingly, all plants regenerated by gynogenesis and durum wheat × maize crosses were green whereas all plants obtained by isolated microspore culture were albino. In the haploid production system, all steps of the process are important for the three methods. The critical steps that have greatly reduced the number of regenerated haploid plants were induction, embryogenesis and regeneration for microspore culture, forming and regeneration of calli or embryo and haploid regeneration for interspecific crosses and gynogenesis. Genotypes with good capacity of induction have not necessarily a good capacity of haploid plantlets regeneration and vice-versa. However, calli or embryos formation seems to be an indicator of the haploid production. Overall, Razzek showed a good ability to produce haploids using the three methods. Each haplomethod showed a specific advantage. Although gynogenesis is the less used method for durum wheat, it has proved to be a successful approach for green haploid plant production.  

scholarly journals Effects of Genotype and Culture Conditions on Microspore Embryogenesis and Plant Regeneration in Brassica Rapa ssp. Rapa L.

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 278 ◽  
Author(s):  
Daria Shumilina ◽  
Dmitry Kornyukhin ◽  
Elena Domblides ◽  
Alexey Soldatenko ◽  
Anna Artemyeva
Keyword(s):  
Microspore Culture ◽  
Doubled Haploids ◽  
Genetic Disorder ◽  
Cold Treatment ◽  
Microspore Embryogenesis ◽  
Culture Conditions ◽  
Secondary Embryogenesis ◽  
Isolated Microspore Culture ◽  
Pure Lines

Turnip is a biennial crop and, consequently, the creation of pure lines for breeding is a time-consuming process. The production of pure turnip lines using doubled haploids produced in isolated microspore culture has not been sufficiently developed. The aim of the present work was to determine some key factors inducing embryogenesis in the isolated microspore culture of turnip, as well as investigating the manners of embryo development. It was shown that the acidity of the medium is an important factor in embryo production; different optimal pH levels ranging from 6.2 to 6.6 corresponded to individual genotypes. Such factors as the cold treatment of buds and the addition of activated charcoal to the nutrient medium increased the responsiveness of all genotypes studied. The turnip variety ‘Ronde witte roodkop herfst’ demonstrated a genetic disorder in the development of microspores; namely, non-separation of some microspores from tetrads. In the in vitro culture, each of the daughter microspores developed on its own. This indicates the dependence of the possibility of embryogenesis in the turnip microspore culture on the genotype. Results suggest that the initiation of secondary embryogenesis in primary embryos leads to an increase in the proportion of doubled haploid plants.

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