scholarly journals Tradition, quality and biotechnology in Hungarian spice pepper ( Capsicum annuum L.) breeding

2010 ◽  
Vol 58 (3) ◽  
pp. 259-266 ◽  
Author(s):  
J. Pauk ◽  
C. Lantos ◽  
G. Somogyi ◽  
P. Vági ◽  
Z. Ábrahám Táborosi ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Culture Methods ◽  
Culture Techniques ◽  
New Genotype ◽  
Breeding Programmes ◽  
History Of ◽  
Dh Lines ◽  
Two Hybrid

Spice pepper production has a history of almost 300 years in the southern part of Hungary. In this study the results of two biotechnological improvements are summarized. Anther and isolated microspore culture techniques were improved to release haploid and doubled haploid (DH) lines for spice pepper breeding. Both the anther and isolated microspore culture methods were successfully used in spice pepper haploid production. Microspore culture-derived structures were analysed to identify their different parts. Green plantlets were regenerated from embryos derived from both anther and microspore cultures. Their doubled haploid analogues were integrated into Hungarian spice pepper hybrid seed breeding programmes. One hybrid, Sláger, was released as a new genotype for spice pepper production in 2008 and two hybrid candidates (Délibáb and Bolero) are now being tested in official trials.

Ploidy of Regenerated Broccoli Derived from Microspore Culture Versus Anther Culture

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 533g-534
Author(s):  
Min Wang ◽  
Mark W. Farnham
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Direct Method ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Chromosome Complement ◽  
Culture Method ◽  
Lower Percentage ◽  
Ploidy Levels ◽  
Culture Techniques

Anther and microspore culture are commonly utilized to produce doubled-haploid (diploid), homozygous lines in broccoli (Brassica oleracea L. Italica Group). It is well-documented that doubled-haploid regenerants are produced by means of polyploidization during anther culture. However, polyploidization may not occur at all, or it may involve a tripling or quadrupling of the chromosome complement. As a consequence, regenerated populations from anther culture contain diploids, but also haploids, triploids, and tetraploids. Microspore culture represents a simpler and more direct method for producing doubled-haploids. Although a similar mix of ploidy types is likely to be observed among regenerants derived from microspore culture, the actual ploidy levels of such regenerants have not been documented for broccoli. Thus, the objectives of this study were to compare ploidy levels of regenerants developed using both anther and microspore culture in broccoli, and to examine phenotypic variation in ploidy makeup of populations developed from both anther and microspore culture using different F1 hybrids. Broccoli regenerants were derived simultaneously from both anther and microspore cultures using the same four F1 hybrids, including Everest, Patriot, Greenbelt and Major. Ploidy level was determined by flow cytometry. A majority of regenerants derived from both anther and microspore culture, were determined to be diploids or tetraploids. Significant differences in ploidy makeup of populations were observed among hybrid varieties for both culture techniques. Regardless of the culture method used, `Everest' produced a greater percentage of diploids and a lower percentage of tetraploids than `Patriot' did. Haploids were observed more frequently from microspore culture than from anther culture when `Everest' and `Major' served as parents.

scholarly journals Retraction Note: Doubled haploid production in alfalfa (Medicago sativa L.) through isolated microspore culture

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dengxia Yi ◽  
Jifeng Sun ◽  
Yanbin Su ◽  
Zongyong Tong ◽  
Tiejun Zhang ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Haploid Production ◽  
Medicago Sativa L

This paper has been retracted.

scholarly journals Improvements of doubled haploid production protocol for white cabbage (Brassica oleracea var. capitata L.)

2018 ◽  
Vol 30 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Katarina Rudolf Pilih ◽  
Urška Karolina Potokar ◽  
Borut Bohanec
Keyword(s):  
Brassica Oleracea ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Petri Dish ◽  
Abnormal Development ◽  
Haploid Induction ◽  
Embryo Formation ◽  
White Cabbage ◽  
Dh Lines ◽  
Further Development

Abstract Protocols leading to the development of doubled haploid (DH) lines by microspore culture are widely used in white cabbage (Brassica oleracea var. capitata L.), but efficiency varies according to the cultivar and induction procedure. Forty different genotypes consisting of F1 cultivars and their crosses with responsive doubled haploid lines were tested to evaluate the androgenic response. In total, 20,032 embryos were produced. On average, the haploid induction response of F1 cultivars was 7.0 embryos/Petri dish, but the average of these hybrids crossed to responsive DH lines was 26.6 embryos/Petri dish. In seven reciprocal crosses, a difference was observed in just one, meaning that the maternal effect probably has a minor influence on haploid embryogenesis in cabbage. Addition of 0.02% activated charcoal (AC) to the induction media increased embryo formation in several low-responsive genotypes, but its effect on embryo formation of high-responsive genotypes was predominantly negative, although larger embryos were formed on media containing AC than without AC. Further development into plantlets was tested by two procedures. Formed embryos were either transferred directly to regeneration medium or treated with abscisic acid and desiccated for 4 weeks. Regrowth and further development reached on average 15.5 and 57.6%, for the first and second procedures, respectively. Plantlets developed by direct transfer often exhibited abnormal development or hyperhydricity, unlike the desiccated embryos. Spontaneous diploidisation of embryos reached 42.5% in total and was not affected by AC added to the induction media.

scholarly journals Agronomic Performance of Doubled Haploid Lines and Pedigree-Derived Lines of Winter Oilseed Rape

2011 ◽  
Vol 40 (No. 4) ◽  
pp. 127-133
Author(s):  
V. Kučera ◽  
E. Schwarzbach ◽  
M. Klíma ◽  
M. Vyvadilová
Keyword(s):  
Oilseed Rape ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Winter Oilseed Rape ◽  
Actual System ◽  
Random Samples ◽  
Breeding Programmes ◽  
Dh Lines ◽  
The Given ◽  
Overlapping Sets

In four not overlapping sets of cultivars, including doubled haploid (DH) lines and pedigree derived (PD) lines of winter oilseed rape, both types of lines were compared in basic agronomic traits. The tested lines originated from breeding programmes of different breeders. Each set of cultivars was tested in a different year from 2001 to 2004 in multi-location trials with four replicates. There was no consistency in yield superiority of DH or PD lines and in all four sets the differences between the means of DH and PD groups were insignificant. On average, the differences between the groups of DH and PD lines were negligible in all evaluated agronomic traits. The statistical analyses of yield performance however revealed a very low predictive value of the multi-location trials. It can be concluded that with the given selection techniques and the actual system of cultivar trials nearly random samples of possible DH and PD lines were obtained in which no superiority of DH or PD lines in the evaluated agronomic traits could be detected.  

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