Hybrid wheat. VII. Tests on the transmission of cytoplasmic male sterility in wheat by embryo-endosperm grafting

Euphytica ◽  
1968 ◽  
Vol 17 (3) ◽  
pp. 439-444 ◽  
Author(s):  
J.-R. Lacadena

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Joanna Melonek ◽  
Jorge Duarte ◽  
Jerome Martin ◽  
Laurent Beuf ◽  
Alain Murigneux ◽  
...  

AbstractHybrid wheat varieties give higher yields than conventional lines but are difficult to produce due to a lack of effective control of male fertility in breeding lines. One promising system involves the Rf1 and Rf3 genes that restore fertility of wheat plants carrying Triticum timopheevii-type cytoplasmic male sterility (T-CMS). Here, by genetic mapping and comparative sequence analyses, we identify Rf1 and Rf3 candidates that can restore normal pollen production in transgenic wheat plants carrying T-CMS. We show that Rf1 and Rf3 bind to the mitochondrial orf279 transcript and induce cleavage, preventing expression of the CMS trait. The identification of restorer genes in wheat is an important step towards the development of hybrid wheat varieties based on a CMS-Rf system. The characterisation of their mode of action brings insights into the molecular basis of CMS and fertility restoration in plants.







2019 ◽  
Vol 60 (7) ◽  
pp. 1604-1618
Author(s):  
Hongxia Li ◽  
Jinglei Guo ◽  
Chengyang Zhang ◽  
Weijun Zheng ◽  
Yulong Song ◽  
...  

Abstract K-type cytoplasmic male sterility (KCMS) lines were ideal material for three-line hybrid wheat system due to the major role in hybrid wheat production. In this study, the morphology of developing microspore and mature pollen was compared between a KCMS line and its near-isogenic restorer line (KCMS-NIL). The most striking difference is that the microspore was unable to develop into tricellular pollen in the KCMS line. MicroRNA plays vital roles in flowering and gametophyte development. Small RNA sequencing identified a total of 274 known and 401 novel miRNAs differentially expressed between two lines or two developmental stages. Most of miRNAs with high abundance were differentially expressed at the uninucleate stage, and their expression level recovered or remained at the binucleate stage. Further degradome sequencing identified target genes which were mainly enriched in transcription regulation, phytohormone signaling and RNA degradation pathways. Combining with the transcriptome data, a correlation was found between the abnormal anther development, such as postmeiotic mitosis cessation, deformative pollen wall and the chromosome condensation of the vegetative cell, and the alterations in the related miRNA and their targets expression profiles. According to the correlation and pathway analysis, we propose a hypothetic miRNA-mediated network for the control of KCMS restoration.



Author(s):  
K.R. KANUKOVA ◽  
◽  
Z.I. BOGOTOVA ◽  
I.Kh. GAZAEV ◽  
S.P. APPAEV ◽  
...  


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