Basic Studies on Hybrid Wheat Breeding : V. Performance of the F1 Hybrids Produced by Utilizing Cytoplasmic Male Sterility

A type of male sterility found in two Desmodium plants of probably interspecific hybrid origin was cytoplasmically inherited. The cytoplasmic male-sterile character was incorporated in the tropical legume Desmodium sandwicense by backcrossing. In this genetic background pollen sterility was complete. The male-sterile character was not graft-transmissible, and it produced no detectable pleiotropic effects on growth and development. Desmodium intortum gave restoration of pollen fertility in Fl hybrids with male-sterile lines of D. sandwicense. Restored F1 hybrids produced apparently normal pollen, but tests of functional ability of the pollen disclosed that pollen fertility was less than that of Fl hybrids with normal cytoplasm. Incomplete restoration of fertility was not due to heterozygosity of fertility-restoring genes with gametophytic expression, since fertility-restoring genes were shown to act sporophytically. The results established the occurrence in the legume Desmodium of a system of determination of the male-sterile, fertility-restored phenotypes that is similar to the cytoplasmic male sterility systems described in many other angiosperm plants. A scheme utilizing the genetic stocks produced in this study for commercial production of the interspecific hybrid D. sandwicense x D. intortum as a cultivar is presented.

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Development of male sterile broccoli lines with raphanus sativus cytoplasm and assessment of their value for breeding purposes

Journal of Horticultural Research ◽

10.2478/johr-2013-0028 ◽

2013 ◽

Vol 21 (2) ◽

pp. 101-107 ◽

Cited By ~ 1

Author(s):

Piotr Kamiński

Keyword(s):

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Raphanus Sativus ◽

Morphological Characters ◽

F1 Hybrids ◽

Vegetable Crops ◽

Male Sterile ◽

Self Compatibility ◽

High Level ◽

Research Institute

ABSTRACT The aim of this study was to obtain new broccoli lines with cytoplasmic male sterility trait for the development of the modern F1 hybrids. CDT70 cauliflower line obtained in the Research Institute of Horticulture, formerly Research Institute of Vegetable Crops, Skierniewice, Poland, with the reliable cytoplasmic male sterility from Raphanus sativus (Ogu-INRA) was selected as a source of this trait. Three broccoli lines: BMi, BCr1 and BCr2 were used as donors of commercial characters in all cross combinations with sterile components. Selected fertile broccoli genotypes were characterized by good quality, uniformity and high level of self-compatibility. The breeding procedure included three consecutive back crosses of male sterile genotypes with fertile broccoli lines that lasted from 2008 to 2012. In each generation, self-compatibility level, the stability of the male sterility trait and ability for the generative propagation of back-crossed genotypes were tested in comparison with donor broccoli lines in the greenhouse. The agronomical and morphological characters of the back-crossed progeny were also evaluated in the field. As a result, three CMS broccoli lines of Bc3 generation with good quality and high seeding index, suitable for the breeding purposes, were obtained.

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Development of thermo-photo sensitive genic male sterile lines in wheat using doubled haploid breeding

10.21203/rs.2.15367/v3 ◽

2020 ◽

Author(s):

Hongsheng Li ◽

Shaoxiang Li ◽

Sedhom Abdelkhalik ◽

Armaghan Shahzad ◽

Jian Gu ◽

...

Keyword(s):

Male Sterility ◽

Doubled Haploid ◽

Yellow Rust ◽

Wheat Breeding ◽

Yield Potential ◽

Limiting Factor ◽

Hybrid Wheat ◽

Male Sterile ◽

Dh Lines ◽

F2 Generation

Abstract Background: Two-line hybrid wheat system using thermo-photo sensitive genic male sterility (TPSGMS) is currently the most promising approach for wheat heterosis utilization in China. However, during past twenty years only few TPSGMS lines were developed in hybrid wheat breeding, which has been the main limiting factor to create heterotic hybrids. Application of doubled haploid (DH) breeding provides a useful strategy to efficiently develop practically usable TPSGMS lines. Results: F1s and selected F2 and F3 sterile plants of eight crosses made from two commercial TPSGMS lines were used to produce DH lines. We developed a total of 24 elite DH sterile lines with stable sterility, good outcrossing and yield potential, resistance to yellow rust and powdery mildew, as well as desirable plant height (50-60 cm). These DH lines were developed within 4 years through at least one year of evaluation. The stability of male sterility was confirmed for most (20/24) of these elite DH sterile lines by multiple tests in two or three years. These lines are expected to be used in hybrid wheat breeding. The percentage of elite lines developed from the tested DH lines produced from filial generations was in the order of F2 > F3 > F1. Conclusions: We demonstrate that coupling DH techniques with conventional breeding is an efficient strategy for accelerating the development of more practical wheat TPSGMS lines. Generation of DHs from F2 generation appeared to be the better choice considering the balance of shortening breeding time and overall breeding efficiency.

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Basic Studies on Hybrid Wheat Breeding : VII. Characteristics of the Male Sterile Lines of Common Wheat Cultivars

Ikushugaku zasshi ◽

10.1270/jsbbs1951.26.179 ◽

1976 ◽

Vol 26 (3) ◽

pp. 179-186 ◽

Cited By ~ 2

Author(s):

Junzo FUJIGAKI ◽

Koichiro TSUNEWAKI

Keyword(s):

Common Wheat ◽

Wheat Breeding ◽

Hybrid Wheat ◽

Wheat Cultivars ◽

Male Sterile ◽

Basic Studies

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The genetic basis of cytoplasmic male sterility and fertility restoration in wheat

Nature Communications ◽

10.1038/s41467-021-21225-0 ◽

2021 ◽

Vol 12 (1) ◽

Cited By ~ 2

Author(s):

Joanna Melonek ◽

Jorge Duarte ◽

Jerome Martin ◽

Laurent Beuf ◽

Alain Murigneux ◽

...

Keyword(s):

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Genetic Basis ◽

Fertility Restoration ◽

Effective Control ◽

Hybrid Wheat ◽

Sequence Analyses ◽

Wheat Varieties ◽

Breeding Lines ◽

Restorer Genes

AbstractHybrid wheat varieties give higher yields than conventional lines but are difficult to produce due to a lack of effective control of male fertility in breeding lines. One promising system involves the Rf1 and Rf3 genes that restore fertility of wheat plants carrying Triticum timopheevii-type cytoplasmic male sterility (T-CMS). Here, by genetic mapping and comparative sequence analyses, we identify Rf1 and Rf3 candidates that can restore normal pollen production in transgenic wheat plants carrying T-CMS. We show that Rf1 and Rf3 bind to the mitochondrial orf279 transcript and induce cleavage, preventing expression of the CMS trait. The identification of restorer genes in wheat is an important step towards the development of hybrid wheat varieties based on a CMS-Rf system. The characterisation of their mode of action brings insights into the molecular basis of CMS and fertility restoration in plants.

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