Extraction of junctional complexes from triad junctions of rabbit skeletal muscle

1994 ◽  
Vol 15 (5) ◽  
pp. 493-504 ◽  
Author(s):  
H. K. Motoike ◽  
A. H. Caswell ◽  
H. M. Smilowitz ◽  
N. R. Brandt
Keyword(s):  
Skeletal Muscle ◽  
Rabbit Skeletal Muscle ◽  
Junctional Complexes ◽  
Triad Junctions

Cryo-Electron Tomography of Isolated Triad Junctions from Skeletal Muscle

2001 ◽  
Vol 7 (S2) ◽  
pp. 94-95 ◽  
Author(s):  
C.-E. Hsieh ◽  
M. Marko ◽  
B.K. Rath ◽  
S. Fleischer ◽  
T. Wagenknecht
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Electron Tomography ◽  
Mass Density ◽  
Tomographic Reconstruction ◽  
Three Dimensional ◽  
Cryo Electron Tomography ◽  
Junctional Complexes ◽  
Triad Junction ◽  
Triad Junctions

In skeletal muscle, depolarization of the plasma membrane, which is initiated at the neuromuscular junction, is transduced to a rise in cytoplasmic calcium at specialized structures known as triad junctions (TJs). TJs occur in the myofiber’s interior at regions near the z-lines, where transversely oriented tubular invaginations of the plasma membrane (T-tubules) form junctions with two elements of the sarcoplasmic reticulum (SR). Isolation of membrane fractions that are enriched in junctional complexes and which retain function has been reported.Figure 1 shows a region of an electron micrograph containing an isolated TJ in the frozen-hydrated state. in the orientation shown, two SR-derived vesicles sandwich a flattened vesicle derived from the T-tubule. The junctional regions contain a complex distribution of density, presumably due to proteins that are known to be present in TJs. Electron tomography offers the means to determine the three-dimensional mass density from such micrographs, which would greatly aid in their interpretation. Only recently has the automated data collection technology for determining tomograms of non-stained, frozen-hydrated specimens become available. Here we describe the first tomographic reconstruction of a frozen-hydrated triad junction by automated electron tomography.

scholarly journals The Reduction in Vitro of 17α-Hydroxypregnenolone (3β,17α-Dihydroxy-Δ5-pregnen-20-one) by Rabbit Skeletal Muscle

1960 ◽  
Vol 235 (10) ◽  
pp. 2797-2800
Author(s):  
Patricia Z. Thomas ◽  
Enrico Forchielli ◽  
Ralph I. Dorfman
Keyword(s):  
Skeletal Muscle ◽  
Rabbit Skeletal Muscle

scholarly journals Characterization of rabbit skeletal muscle glycogenin. Tyrosine 194 is essential for function

1993 ◽  
Vol 268 (20) ◽  
pp. 14687-14693 ◽  
Author(s):  
Y. Cao ◽  
A.M. Mahrenholz ◽  
A.A. DePaoli-Roach ◽  
P.J. Roach
Keyword(s):  
Skeletal Muscle ◽  
Rabbit Skeletal Muscle
Sign in / Sign up

Export Citation Format

Share Document