Conversion of D-xylose into ethanol by the yeast Pachysolen tannophilus

Olive oil mill solid residue (OMSR) is the solid waste generated during olive oil production process in three-phase olive mills. It consists of the remaining pulp of olive processing after the extraction of oil, as well as the cracked seeds of the olive fruits, containing thus mainly lignocellulose and residual oil. The commonly used practice for OMSR management is combustion, after having extracted the residual oil by secondary extraction using organic solvents. Other proposed ways of OMSR management are their exploitation as substrate for edible fungi production and compost, and as feedstock for biofuels generation such as methane and bioethanol. In the latter case, the complex carbohydrates (cellulose and hemicellulose) of the lignocellulose of OMSR have to be degraded towards their simple sugars and further fermented via microorganisms. The purpose of the present study was to investigate the effect of thermochemical pre-treatment of OMSR, on the final ethanol yield from the yeast Pachysolen tannophilus. Nine different types of OMSR-based substrates were tested i.e. raw OMSR, hydrolysates generated from pretreated OMSR with NaOH (0.5 %, 1.5 % w/v) and H2SO4 (0.5 %, 1.5 % v/v), and pretreated OMSR with NaOH (0.5 %, 1.5 % w/v) and H2SO4 (0.5 %, 1.5 % v/v) whole biomass. It was shown that in all cases pretreatment enhanced the consumption of carbohydrates as well as ethanol final yields.

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The influence of the initial concentrations of glucose and yeast extract on the ethanolic fermentation by Pachysolen tannophilus

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19900854 ◽

1990 ◽

Vol 55 (3) ◽

pp. 854-866 ◽

Cited By ~ 5

Author(s):

Rodríguez V. Bravo ◽

Rubio F. Camacho ◽

Villasclaras S. Sánchez ◽

Vico M. Castro

Keyword(s):

Cell Growth ◽

Ethanol Production ◽

Yeast Extract ◽

Culture Medium ◽

Ethanolic Fermentation ◽

Pachysolen Tannophilus ◽

Significant Component ◽

Batch Cultures

The ethanolic fermentation in batch cultures of Pachysolen tannophilus was studied experimentally varying the initial concentrations of two of the components in the culture medium: glucose between 0 and 200 g l-1 and yeast extract between 0 and 8 g l-1. The yeast extract appears to be a significant component both in cell growth and for ethanol production.

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Prospective evaluation of xylitol production using Dabaryomyces hansenii var hansenii, Pachysolen tannophilus, and Candida guillermondii with sustainable agricultural residues

Biomass Conversion and Biorefinery ◽

10.1007/s13399-020-01221-y ◽

2021 ◽

Author(s):

P. Saravanan ◽

S. Ramesh ◽

N. Jaya ◽

S. Anuradha Jabasingh

Keyword(s):

Agricultural Residues ◽

Xylitol Production ◽

Prospective Evaluation ◽

Pachysolen Tannophilus

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Effects of Nitrate on Fermentation of Xylose and Glucose by Pachysolen Tannophilus

Nature Biotechnology ◽

10.1038/nbt0883-503 ◽

1983 ◽

Vol 1 (6) ◽

pp. 503-506 ◽

Cited By ~ 13

Author(s):

Thomas W. Jeffries

Keyword(s):

Pachysolen Tannophilus

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Comparative genomics of biotechnologically important yeasts

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1603941113 ◽

2016 ◽

Vol 113 (35) ◽

pp. 9882-9887 ◽

Cited By ~ 165

Author(s):

Robert Riley ◽

Sajeet Haridas ◽

Kenneth H. Wolfe ◽

Mariana R. Lopes ◽

Chris Todd Hittinger ◽

...

Keyword(s):

Gene Clusters ◽

Xylose Utilization ◽

Comparative Genome Analysis ◽

Pachysolen Tannophilus ◽

Biochemical Traits ◽

Code Change ◽

Mating Type Switching ◽

Yeast Phylogeny ◽

Genomic Basis ◽

Insight Into

Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the clade sister to the known CUG-Ser clade. Our well-resolved yeast phylogeny shows that some traits, such as methylotrophy, are restricted to single clades, whereas others, such as l-rhamnose utilization, have patchy phylogenetic distributions. Gene clusters, with variable organization and distribution, encode many pathways of interest. Genomics can predict some biochemical traits precisely, but the genomic basis of others, such as xylose utilization, remains unresolved. Our data also provide insight into early evolution of ascomycetes. We document the loss of H3K9me2/3 heterochromatin, the origin of ascomycete mating-type switching, and panascomycete synteny at the MAT locus. These data and analyses will facilitate the engineering of efficient biosynthetic and degradative pathways and gateways for genomic manipulation.

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