Tritium Incorporation From Tritium Water as an Index of Metabolism

<p>Part A The metabolism of mustard (Sinapis alba) seeds wet at sub-germination temperatures has been studied using tritium incorporation as an index of metabolism. The theory and scope of the method are discussed. The enzymic reactions known in 1964 are surveyed one by one, suggesting which will, or will not, incorporate tritium from THO into specified metabolites, or cannot be confidently predicted either way. Improvements have been made in the chromatography procedure. At 0°, many of the normal germination chemical reactions proceed, but about one tenth as fast as at 24°. Amino-acids are being metabolised within 2 h of wetting the seeds, and malic and citric acids within 4 h. Within 24 h lipids and fructose are undergoing reactions. An unidentified compound “M”, not reported in normal germination, is being metabolised within 48 h. Another aberration from normal is the absence of detectable succinate metabolism. Labelling of the solid residue (insoluble in ethanol and in water) always occurs, shown to be largely non-metabolic. To explain the non-germination of seeds at temperatures near 0°, it is hypothesized that the Krebs cycle is qualitatively altered, perhaps by “wasting away” of glutamate to 4-aminobutyrate instead of its routing into the Krebs cycle as alpha-oxoglutarate. Part B A method has been developed for studying the metabolism of dry seeds, spores and pollen by exposure to THO vapour. Dry Pinus radiata pollen labels many compounds. A few have been identified and are common metabolites. It may be that the metabolism of dry pollen is not qualitatively different from its germination reactions. Dry mustard seeds and spores of the fungus Pithomyces chartarum give, in contrast to pollen, patterns of incorporation very different from those in early germination.</p>

Tritium Incorporation From Tritium Water as an Index of Metabolism

<p>Part A The metabolism of mustard (Sinapis alba) seeds wet at sub-germination temperatures has been studied using tritium incorporation as an index of metabolism. The theory and scope of the method are discussed. The enzymic reactions known in 1964 are surveyed one by one, suggesting which will, or will not, incorporate tritium from THO into specified metabolites, or cannot be confidently predicted either way. Improvements have been made in the chromatography procedure. At 0°, many of the normal germination chemical reactions proceed, but about one tenth as fast as at 24°. Amino-acids are being metabolised within 2 h of wetting the seeds, and malic and citric acids within 4 h. Within 24 h lipids and fructose are undergoing reactions. An unidentified compound “M”, not reported in normal germination, is being metabolised within 48 h. Another aberration from normal is the absence of detectable succinate metabolism. Labelling of the solid residue (insoluble in ethanol and in water) always occurs, shown to be largely non-metabolic. To explain the non-germination of seeds at temperatures near 0°, it is hypothesized that the Krebs cycle is qualitatively altered, perhaps by “wasting away” of glutamate to 4-aminobutyrate instead of its routing into the Krebs cycle as alpha-oxoglutarate. Part B A method has been developed for studying the metabolism of dry seeds, spores and pollen by exposure to THO vapour. Dry Pinus radiata pollen labels many compounds. A few have been identified and are common metabolites. It may be that the metabolism of dry pollen is not qualitatively different from its germination reactions. Dry mustard seeds and spores of the fungus Pithomyces chartarum give, in contrast to pollen, patterns of incorporation very different from those in early germination.</p>

Influence of Crystallite Size of Nickel and Cobalt Ferrites on the Catalytic Pyrolysis of Buckwheat Straw by Using TGA-FTIR Method

Pyrolysis of buckwheat straw with or without catalysts was investigated using the TGA-FTIR method to determine the influence of nickel and cobalt ferrites on the distribution of pyrolysis products. According to the obtained results, the overall shape of the thermogravimetric and derivative thermogravimetric curves is unchanged in the presence of nickel and cobalt ferrites but different weight losses were observed. All catalysts contribute to the formation of solid residue from BWS pyrolysis. The presence of cobalt ferrites exhibited the highest bio-oil yields, whereas the highest non-condensable gas yield and the lowest bio-oil yield was obtained with the addition of NiFe2O4 (1) catalyst. According to the obtained results, the ability of nickel and cobalt ferrites to catalyze deoxygenation reactions depends on the crystallite size. The nickel or cobalt ferrites with smaller crystallite size (15-22 nm) show a higher ability to catalyzed dehydration reaction than catalysts with larger crystallite size (45-54 nm).

INVESTIGATION OF THE PROCESSES OF THERMAL DESTRUCTION OF CELLULOSE-CONTAINING (PAPER) WASTE

Materials of practical research work on thermal destruction of paper waste were presented. The main task was The comprehensive study of the aspects of carbon formation on the basis of analytical studies was considered, as well as using a specially built laboratory installation — a waste graphitizer. Research has been carried out on the effectivity of application of pyrolysis gases of the process as fuel to maintain the temperatures of the thermal destruction reaction. Practical examples have proved the possibility and expediency of using the solid residue of the reaction as a component in various fields of production. Bibl. 10, Fig. 1, Tab. 3.

KINETIC STUDY ON HYDROTHERMAL COMPOSITION OF GLUCOSE IN NAOH SOLUTION WITH ZNO AS CATALYST

<p>Hydrothermal liquifaction is a biomass conversion process, where the structure of the biomass is convert into liquid components under super critical conditions with a high temperature. In this study, glucose is used as biomass. The purpose of this study was to study the reaction kinetics and determine the hydrothermal decomposition of glucose in NaOH solution. This experiment used 10 grams of glucose and dissolve it in 80 mL of NaOH solution then put it in an autoclave. Experiments were carried out by varying the heating temperature carried out in an autoclave with a magnetic stirrer. After heating at various temperatures, the autoclave is immediately cooled down. The processed material is filtered to separate insoluble solids from the liquid phase. The solid residue that has been separated from the liquid phase is then dried in an oven at 105°C for 24 hours. The composition of the filtrate was analyzed using the GC-MS method and the glucose concentration was analyzed using the Lane Eynon method. Prior to GC-MS analysis, the filtrate was distilled at atmospheric pressure until a solid residue remained. The sample analyzed is the result of distillation with a temperature above 100°C to ensure that there is no water and residual glucose in the sample. The results of GC-MS analysis of product samples from the hydrothermal decomposition process had 3 peaks. The first peak shows the compound 1,3 Dipalmitin which has an area of 14.74%, the second peak shows the Olealdehyde compound which has an area of 32.35%, and the third peak shows the 1,2-Epoxyhexadecane compound which has an area of 52.91%. The kinetics results in hydrothermal decomposition of glucose in this experiment obtained a reaction order of 2 with an activation energy (Ea) of 15.91 KJ / mol and a pre-exponential factor of 66.12.</p>