Minimal growth in vitro conservation of coffee (Coffea spp.)

1991 ◽  
Vol 27 (3) ◽  
pp. 333-339 ◽  
Author(s):  
Anna Bertrand-Desbrunais ◽  
Michel Noirot ◽  
Andr� Charrier
Keyword(s):  
In Vitro Conservation ◽  
Minimal Growth

scholarly journals IN VITRO CONSERVATION OF DATE PALM SHOOT TIP EXPLANTS UNDER MINIMAL GROWTH CONDITION

2013 ◽  
Vol 91 (3) ◽  
pp. 1043-1062
Author(s):  
MAIADA M. EL-DAWAYATI ◽  
ELSAYED I. BAKER ◽  
AMINA H. GOMAA ◽  
ZEINAB E. ZAYED
Keyword(s):  
Growth Condition ◽  
Date Palm ◽  
Shoot Tip ◽  
In Vitro Conservation ◽  
Minimal Growth ◽  
Shoot Tip Explants

scholarly journals In Vitro Conservation of Medicinal Plant St. John’s Wort (Hypericum perforatum L.) Through Dilution of Basic Medium

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Sitti Fatimah Syahid
Keyword(s):  
Hypericum Perforatum ◽  
In Vitro Conservation ◽  
Basic Medium ◽  
Minimal Growth ◽  
St John’S Wort ◽  
St John's Wort ◽  
Necrotic Symptom ◽  
Hypericum Perforatum L ◽  
Culture Growth

In vitro techniques could be applied for plant conservation through minimal growth. Growth reduction is generally attained by modifying the culture medium and/or the environmental condition. Conservation of St. John’s wort (Hypericum perforatum L.) in vitro through dilution of basic medium was conducted at the Tissue Culture Laboratory of Indonesian Spice and Medicinal Crops Research Institute (ISMCRI) in 2018. Sterile shoots were cultured into Murashige and Skoog (MS) medium by reducing macronutrients. The treatments used were full-MS + 0.1 mg L-1 N6 - benzyladenine (BA) as control; ¾ MS + 0.1 mg L-1 BA; ½ MS + 0.1 mgL-1 BA and ¼ MS + 0.1 mg L-1 BA. Observation of the culture growth was conducted three months after the treatments. The experiment was arranged in Completely Randomized Design with ten replications. The result showed that the use of a dilution of basic medium affected the growth of St. John’s wort. The use of ¼ MS + 0.1 mg L-1 BA composition could suppress culture growth (number of shoots, shoots length and number of leaves), without showing necrotic symptom until three months of conservation. This treatment could be used as an alternative to minimize the culture of St. John’s wort for in vitro conservation.

scholarly journals In Vitro Techniques to the Conservation and Plant Regeneration of Malanga (Colocasia esculenta L. Schott)

HortScience ◽  
2019 ◽  
Vol 54 (3) ◽  
pp. 514-518 ◽  
Author(s):  
Eucario Mancilla-Álvarez ◽  
Marco A. Ramírez-Mosqueda ◽  
Samantha Arano-Avalos ◽  
Rosalía Núñez-Pastrana ◽  
Jericó J. Bello-Bello
Keyword(s):  
Genetic Resource ◽  
In Vitro Regeneration ◽  
Colocasia Esculenta ◽  
In Vitro Conservation ◽  
Plant Genetic Resource ◽  
Minimal Growth ◽  
Shoot Number ◽  
Number Of Leaves ◽  
Improvement Programs

Malanga (Colocasia esculenta) is a plant genetic resource that requires biotechnological strategies for conservation and propagation. One time-, labor-, and space-saving option is in vitro conservation and regeneration. The objective of this study was to develop a protocol for in vitro regeneration and conservation of germplasm of C. esculenta var. criolla. For conservation through minimal growth, we assessed several concentrations of Murashige and Skoog (MS) medium (one-third, one-half, and three-quarter strength), the growth retardant ancymidol (0, 1, 2, and 3 mg·L−1), and the osmoregulator polyethylene glycol (PEG-8000 mw) at different concentrations (0, 10, 20, and 30 g·L−1). For in vitro conservation, the percent survival, shoot number and length, and number of leaves and roots per explant were evaluated after 24 weeks. For in vitro regeneration, different concentrations of thidiazuron (TDZ: 0, 0.5, 1, 1.5, and 2 mg·L−1) and 6-benzylaminopurine (BAP; 0, 1, 2, 3, and 4 mg·L−1) were evaluated. After 4 weeks of cultivation, the percent response, shoot number, and number of leaves per explant were recorded. During in vitro conservation, it was noted that the treatment including 2 mg·L−1 ancymidol resulted in a retarded development, without affecting the survival of the C. esculenta germplasm. With regard to shoot regeneration, 7.60 shoots per explant were obtained using 2 mg·L−1 TDZ. Finally, 98% survival was achieved during the acclimatization process. This study will contribute to the establishment of genetic improvement programs through in vitro conservation and propagation of this valuable plant genetic resource.

scholarly journals In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

2019 ◽  
Vol 43 ◽  
Author(s):  
Tecla dos Santos Silva ◽  
Cristina Ferreira Nepomuceno ◽  
Taliane Leila Soares ◽  
José Raniere Ferreira de Santana
Keyword(s):  
Aerial Part ◽  
Dry Mass ◽  
Growth Conditions ◽  
In Vitro Conservation ◽  
Ex Situ ◽  
Minimal Growth ◽  
Survival Percentage ◽  
Medicinal Substances ◽  
Osmotic Agents

ABSTRACT Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.

scholarly journals In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation

2017 ◽  
Vol 52 (12) ◽  
pp. 1286-1290 ◽  
Author(s):  
Hugo Teixeira Gomes ◽  
Patrícia Monah Cunha Bartos ◽  
Maíra Teixeira de Andrade ◽  
Raphael Ferreira Almeida ◽  
Luciana Florencio de Lacerda ◽  
...  
Keyword(s):  
Large Scale ◽  
Growth Conditions ◽  
In Vitro Conservation ◽  
Nodal Segments ◽  
Minimal Growth

Abstract: The objective of this work was to evaluate the micropropagation of blackberry (Rubus spp.) cultivars, after in vitro conservation under minimal growth conditions. Nodal segments of the 'Guarani', 'Caingangue', 'Ébano', and 'Xavante' genotypes were conserved under minimal growth conditions at 20ºC, for 15 months. Microshoots were regenerated and multiplied by up to five successive subcultures, when they were rooted and acclimatized. After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication.

scholarly journals Respon Pertumbuhan Kultur In Vitro Jeruk Besar (Citrus maxima (Burm.) Merr.) cv. Nambangan terhadap Osmotikum dan Retardan

2015 ◽  
Vol 5 (1) ◽  
pp. 21
Author(s):  
Iswari S. Dewi ◽  
Gani S. Jawak ◽  
Bambang S. Purwoko ◽  
M. Sabda
Keyword(s):  
Active Compound ◽  
Growth Response ◽  
In Vitro Conservation ◽  
Green Leaf ◽  
In Vitro Growth ◽  
Minimal Growth ◽  
Plant Materials ◽  
Inhibition Of Growth ◽  
Citrus Maxima

<p>ABSTRACT</p><p>In vitro  conservation has been applied to many species. However, the suppression of explant growth is essential for extending the duration of conservation.  The objective of the research was  to study  in  vitro  growth response  of  pummelo  cv.  Nambangan  to  conservation  medium containing osmotically  active  compound  (osmoticum)  or  growth  suppressant (retardant).  Two  sets  of experiments were conducted  using randomized complete design and replicated three times.  In vitro shoot with four leaves from pummelo, namely cultivar Nambangan, were used as the plant materials. The treatment in the first experiment was  MS + osmoticum (mannitol  0, 20, 40,  and 60 g L-1) and  in the  second  experiment  was  MS +  retardant  (paclobutrazol  0,  1,  3  and  5  mg  L-1).  The  resultsindicated that senescence of the leaf was induced by 20, 40, and 60 g L-1of mannitol. The best media in inhibition of growth  for  pummelo cv. Nambangan was MS + paclobutrazol  1  mg  L-1.  With this media, plant was inhibited but grew normally with green leaf and root.</p><p>Keywords: mannitol, minimal growth, paclobutrazol, pummelo</p><p> </p><p>ABSTRAK</p><p>Konservasi  in  vitro   sudah  banyak  dilakukan  pada  berbagai  spesies. Penghambatan  pertumbuhansangat  penting  bagi  lamanya  tanaman  dapat disimpan.  Tujuan  penelitian  ini  adalah  mempelajari respon  pertumbuhan  in vitro  pamelo  cv.  Nambangan  terhadap  media  konservasi mengandung osmotikum  atau  penghambat  pertumbuhan  (retardan).  Dua percobaan  dilakukan  terpisah  menggunakan  rancangan  acak  lengkap  dan diulang  3  kali.  Tunas  hasil  perbanyakan  in  vitro  dengan  4 daun, digunakan  sebagai  eksplan.  Perlakuan  pada  percobaan  pertama  adalah  MS +  osmotikum (mannitol 0, 20, 40, 60 g L-1) dan pada percobaan kedua adalah MS + retardan (paclobutrazol 0, 1, 3,5 mg L-1). Hasil menunjukkan bahwa daun mengalami senesen oleh perlakuan mannitol. Media yang direkomendasikan untuk konservasi pamelo cv. Nambangan  adalah  MS + paclobutrazol 1 mg  L-1. Dengan media tersebut pertumbuhan dihambat, tetapi tetap normal, berakar dengan daun tetap hijau.</p><p>Kata kunci: mannitol, pertumbuhan minimal, paclobutrazol, pamelo</p>

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