Thiamine pyrophosphatase activity in secretory cells of the lateral prostate and seminal vesicle of normal and castrated guinea pigs and castrates treated with oestradiol

1993 ◽  
Vol 25 (1) ◽  
pp. 77-85 ◽  
Author(s):  
C. C. Tam ◽  
Y. C. Wong
Keyword(s):  
Seminal Vesicle ◽  
Guinea Pigs ◽  
Secretory Cells ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

Improved localization of thiamine pyrophosphatase activity in mounted cryostat sections using gel fixation and gel incubation media

1978 ◽  
Vol 56 (3-4) ◽  
pp. 345-347 ◽  
Author(s):  
Zdeněk Lukáš ◽  
Karel Dvořák ◽  
Zdeňka Juránková
Keyword(s):  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity ◽  
Cryostat Sections

Intracellular digestion and cellular defecation in a monogenean, Diclidophora merlangi

Parasitology ◽  
1975 ◽  
Vol 70 (3) ◽  
pp. 331-340 ◽  
Author(s):  
D. W. Halton
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Digestive Enzymes ◽  
Host Protein ◽  
Blood Proteins ◽  
Host Fish ◽  
Intracellular Digestion ◽  
Thiamine Pyrophosphatase ◽  
Lysosomal System ◽  
Pyrophosphatase Activity

The ultrastructural and cytochemical changes accompanying intracellular digestion and cellular defecation in the haematin cell of Diclidophora merlangi have been described. Blood proteins of the host-fish are sequestered by endocytosis and degraded within an interconnecting network of channels that form an integral, but changing, part of the cell. The digestive enzymes involved originate in the granular endoplasmic reticulum and are packaged in the Golgi apparatus and transferred to the channels in Golgi vesicles. The rate of haemoglobin absorption and the activity of the Golgi, as judged by vesicle counts and staining intensities for thiamine pyrophosphatase activity, are stimulated by the introduction of host protein into the gut lumen. The haematin residues of digestion are extruded periodically into the lumen by exocytosis involving membrane fusion. The process is a continuous one and, in worms starved of food, can result in the complete evacuation of pigment from the cell. It is suggested that a lysosomal system operates in the digestive cycle of the haematin cell.

Localization of Enzymes in Certain Secretory Cells of Helix Tentacles

1964 ◽  
Vol s3-105 (69) ◽  
pp. 49-60
Author(s):  
NANCY J. LANE
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Cell Types ◽  
Secretory Cells ◽  
Oval Cells ◽  
Cytoplasmic Localization ◽  
Cell Organelles ◽  
Distinct Cell ◽  
Thiamine Pyrophosphatase ◽  
Ultrastructural Appearance

Secretory cells in the optic tentacles of the snails, Helix aspersa and H. pomatia, have been investigated for the cytoplasmic localization of certain enzymes. The collar cells, considered to be neurosecretory, and the lateral oval cells, were those examined. Acid phosphatase activity is found in the cytoplasm of both cells, in scattered spheroids called the β-bodies. This enzymatic activity indicates that the β-bodies may be lysosomes, as does their ultrastructural appearance. In the 2 cell types, the activity of both alkaline phosphatase and thiamine pyrophosphatase is localized in crescentic bodies considered to correspond to the Golgi lamellae, and in some of the β-bodies. The latter enzyme also exists in the cortices of the α-bodies which, like the β-bodies, are lipid-containing globules. The activity of both cytochrome oxidase and succinate dehydrogenase is found, not only in granules, rods, and filaments interpreted as the mitochondria, but also on the cortices of some or all of the β-bodies. It is concluded that in invertebrates, the lipochondria may be the sites of activity of many different enzymes which in vertebrates are restricted to distinct cell organelles.

Surface Features of Epithelium of the Seminal Vesicle in Young Adult and Old Guinea Pigs and after Castration

1983 ◽  
Vol 116 (3) ◽  
pp. 257-264 ◽  
Author(s):  
Y.C. Wong
Keyword(s):  
Young Adult ◽  
Seminal Vesicle ◽  
Guinea Pigs ◽  
Surface Features

Ultrastructural localization of thiamine pyrophosphatase activity in the intestinal epithelial cells of adult rats

1984 ◽  
Vol 74 (2) ◽  
pp. 181-187 ◽  
Author(s):  
Yukio Oomori ◽  
Kazuyuki Ono ◽  
Katsushi Ishikawa ◽  
Yoh-ich Satoh ◽  
Mitsuaki Matoba
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Ultrastructural Localization ◽  
Intestinal Epithelial ◽  
Adult Rats ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

Histochemical evaluation of thiamine pyrophosphatase activity during first molar odontogenesis of the neonatal hairless mouse

1977 ◽  
Vol 60 (1) ◽  
pp. 78-90 ◽  
Author(s):  
Brian L. Kuyatt ◽  
Leslie P. Gartner ◽  
James L. Hiatt ◽  
D. Vincent Provenza
Keyword(s):  
Hairless Mouse ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

Redistribution and recycling of internalized membrane in seminal vesicle secretory cells

1990 ◽  
Vol 68 (3) ◽  
pp. 183-193 ◽  
Author(s):  
Lucinda R. Mata ◽  
Erik I. Christensen
Keyword(s):  
Seminal Vesicle ◽  
Secretory Cells

scholarly journals AN ULTRACYTOCHEMICAL AND ULTRASTRUCTURAL STUDY OF EPITHELIAL CELLS IN DUCTULI EFFERENTES OF CHINESE HAMSTER

1971 ◽  
Vol 19 (12) ◽  
pp. 766-774 ◽  
Author(s):  
MASAO YOKOYAMA ◽  
JEFFREY P. CHANG
Keyword(s):  
Epithelial Cells ◽  
Golgi Apparatus ◽  
Phosphatase Activity ◽  
Plasma Membranes ◽  
Chinese Hamster ◽  
Fluid Composition ◽  
Ciliated Cells ◽  
Efferent Duct ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

Ultrastructural and ultracytochemical features of the efferent duct of Chinese hamster have been studied. The ducts are composed of two main types of epithelial cells, ciliated and nonciliated. Distinct structural and cytochemical characteristics of these cells are apparent. Presence of fibrogranular complex which is supposedly related to basal body replication was demonstrated in ciliated cells for the first time in this tissue. Thiamine pyrophosphatase activity in Golgi apparatus, acid phosphatase activity in Golgi apparatus and lysosomes and alkaline phosphatase activity on basal plasma membranes of both ciliated and nonciliated cells have been localized. However, thiamine pyrophosphatase activity was seen only on the luminal surface, apical vacuole and apical tubular structure of nonciliated cells but not on the surface of ciliated cells. Similarly, horseradish peroxidase was absorbed only by nonciliated cells. The cytochemical and ultrastructural differences between the two types of cells indicate a functional specialization. The results indicate that the ciliated cells are concerned with the transportation of the sperms and that the nonciliated cells are concerned with the regulation of fluid composition in the duct since the latter are capable of both secretion and absorption.

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