Inhibition of the contraction of the isolated longitudinal muscle of the guinea-pig ileum by botulinum C2 toxin: Evidence for a role of G/F-actin transition in smooth muscle contraction

1989 ◽  
Vol 340 (3) ◽  
Author(s):  
Stefan Mauss ◽  
Gertrud Koch ◽  
VolkerA.W. Kreye ◽  
Klaus Aktories
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscle Contraction ◽  
Longitudinal Muscle ◽  
Smooth Muscle Contraction ◽  
Guinea Pig Ileum ◽  
C2 Toxin

scholarly journals Efeito do hexametônio sobre a contratilidade de músculo liso de cobaios provocada pelo extrato aquoso do fungo Ramaria Flavo Brunnescens

1986 ◽  
Vol 8 (8) ◽  
pp. 67
Author(s):  
Ana Maria Chagas ◽  
Zuleica Tabarelli ◽  
Ruben Boelter ◽  
Lisandre Kipper ◽  
Rejane Mello Flores ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscle Contraction ◽  
Aqueous Extract ◽  
Smooth Muscle Contraction ◽  
Southern Brazil ◽  
Guinea Pig Ileum ◽  
Strain Transducer ◽  
Fungus Extract ◽  
The Difference

Animals poisoning by ingestion of Ramaria flava brunnescens fungus found in Eucalipto groves is common in Southern Brazil. This poisoning does not have an effective antidote yet and it is common to avoid the toxicity by removing the animals from these fields or by using atropin when fungus intoxication signals appear. Present work was reallized to elucidate the manner and place of action of this fungus aqueous extract on isolated guinea pig ileum. For this purpose, we used Magnus Bath and strain transducer to Physiograph connect. Experiences were realized with 18 ileum tested with 10 mcg Nicotine. 24.600 mcg Ramaria flavo brunnescens fungus' aqueous extract and 30 mcg Hexametonium. Those concentrations were choosen from pilot experiences. The results showed that uses of this fungus extract provokes smooth muscle contraction similar to that of nicotin, the difference between both occurs due to Hexametone blockade impossibility. This fact suggests that the death mechanism of animals that eat this fungus is not due to ganglionar stimulation.

Role of Rho-kinase in guinea-pig gallbladder smooth muscle contraction

2006 ◽  
Vol 534 (1-3) ◽  
pp. 210-217 ◽  
Author(s):  
Teresa Quinn ◽  
Ronan Feighery ◽  
Alan William Baird
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscle Contraction ◽  
Rho Kinase ◽  
Smooth Muscle Contraction

Contraction of guinea pig ileal smooth muscle by acetyl glyceryl ether phosphorylcholine

1981 ◽  
Vol 241 (3) ◽  
pp. C130-C133 ◽  
Author(s):  
S. R. Findlay ◽  
L. M. Lichtenstein ◽  
D. J. Hanahan ◽  
R. N. Pinckard
Keyword(s):  
Biological Activity ◽  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscle Contraction ◽  
Glyceryl Ether ◽  
Platelet Activating Factor ◽  
Smooth Muscle Contraction

Acetyl glyceryl ether phosphorylcholine (AGEPC) is a chemical that has the biological activity of what was formerly termed platelet-activating factor. We report here that synthetic AGEPC induces the contraction of guinea pig ileal smooth muscle. Antagonists of histamine, acetylcholine, and slow-reacting substances (SRS) do not block AGEPC-induced contraction. These responses were long lasting, resistant to washing, and displayed complete agonist specific desensitization. Histamine- and SRS-induced contractions were unaffected by AGEPC. These studies show that AGEPC has the potential to produce a component of anaphylactically induced smooth muscle contraction.

PKC δ-isoform translocation and enhancement of tonic contractions of gastrointestinal smooth muscle

2007 ◽  
Vol 292 (3) ◽  
pp. G887-G898 ◽  
Author(s):  
Daniel P. Poole ◽  
John B. Furness
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscles ◽  
Specific Inhibitor ◽  
Smooth Muscle Contraction ◽  
Enteric Neurons ◽  
Guinea Pig Ileum ◽  
Tonic Component ◽  
Gastrointestinal Smooth Muscle ◽  
Immunohistochemical Evidence

PKC is involved in mediating the tonic component of gastrointestinal smooth muscle contraction in response to stimulation by agonists for G protein-coupled receptors. Here, we present pharmacological and immunohistochemical evidence indicating that a member of the novel PKC isoforms, PKC-δ, is involved in maintaining muscarinic receptor-coupled tonic contractions of the guinea pig ileum. The tonic component of carbachol-evoked contractions was enhanced by an activator of conventional and novel PKCs, phorbol 12,13-dibutyrate (PDBu; 200 nM or 1 μM), and by an activator of novel PKCs, ingenol 3,20-dibenzoate (IDB; 100 or 500 nM). Enhancement was unaffected by concentrations of bisindolylmaleimide I (BIM-I; 22 nM) that block conventional PKCs or by a PKC-ε-specific inhibitor peptide but was attenuated by higher doses of BIM-I (2.2 μM). Relevant proteins were localized at a cellular and subcellular level using confocal analysis. Immunohistochemical staining of the ileum showed that PKC-δ was exclusively expressed in smooth muscles distributed throughout the layers of the gut wall. PKC-ε immunoreactivity was prominent in enteric neurons but was largely absent from smooth muscle of the muscularis externa. Treatment with PDBu, IDB, or carbachol resulted in a time- and concentration-dependent translocation of PKC-δ from the cytoplasm to filamentous structures within smooth muscle cells. These were parallel to, but distinct from, actin filaments. The translocation of PKC-δ in response to carbachol was significantly reduced by scopolamine or calphostin C. The present study indicates that the tonic carbachol-induced contraction of the guinea pig ileum is mediated through a novel PKC, probably PKC-δ.

scholarly journals Role of various kinases in vacular smooth muscle contraction: Study with the kinase inhibitors N40344, ML-9 and H-?

1989 ◽  
Vol 49 ◽  
pp. 300
Author(s):  
Hiroko Takahashi ◽  
Toyokazu Hiranuma ◽  
Mitsugu Harchisa ◽  
Masao Koyama ◽  
Kazuhiro Kohama
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Kinase Inhibitors ◽  
Smooth Muscle Contraction
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