A 7-kDa pollen coating-borne peptide from Brassica napus interacts with S-locus glycoprotein and S-locus-related glycoprotein

Planta ◽  
1995 ◽  
Vol 196 (2) ◽  
Author(s):  
SimonJ. Hiscock ◽  
James Doughty ◽  
AntonyC. Willis ◽  
HughG. Dickinson
Keyword(s):  
Brassica Napus ◽  
S Locus ◽  
Pollen Coating ◽  
S Locus Glycoprotein

Sequence and expression of endogenous S-locus glycoprotein genes in self-compatible Brassica napus

1994 ◽  
Vol 242 (2) ◽  
pp. 209-216 ◽  
Author(s):  
Laurian S. Robert ◽  
Sharon Allard ◽  
Tanya M. Franklin ◽  
Martin Trick
Keyword(s):  
Brassica Napus ◽  
S Locus ◽  
S Locus Glycoprotein

scholarly journals Molecular characterization of the S locus in two self-incompatible Brassica napus lines.

1996 ◽  
Vol 8 (12) ◽  
pp. 2369-2380 ◽  
Author(s):  
K Yu ◽  
U Schafer ◽  
T L Glavin ◽  
D R Goring ◽  
S J Rothstein
Keyword(s):  
Brassica Napus ◽  
Molecular Characterization ◽  
S Locus

scholarly journals Characterization of S haplotype in a new self-compatible Brassica rapa cultivar Dahuangyoucai

2013 ◽  
Vol 49 (No. 4) ◽  
pp. 157-163 ◽  
Author(s):  
X. Zhang ◽  
C. Ma ◽  
D. Yin ◽  
W. Zhu ◽  
C. Gao ◽  
...  
Keyword(s):  
Brassica Species ◽  
Functional Class ◽  
S Locus ◽  
S Haplotype ◽  
Self Compatibility ◽  
Female Determinant ◽  
First Time ◽  
S Locus Glycoprotein ◽  
Signalling Cascade

The most important Brassica species, B. rapa, is naturally self-incompatible. Self-compatible mutants would be useful for dissecting the molecular mechanism of self-incompatibility (SI), a process that promotes outcrossing by recognizing and refusing self-pollens. The S haplotype in a new self-compatible B. rapa cultivar, Dahuangyoucai, was characterized for the first time in this study. Sequence analysis of the S-locus genes, SLG (S-locus glycoprotein), SRK (S-locus receptor kinase) and SCR (S-locus cysteine-rich protein) revealed that Dahuangyoucai contained S haplotype highly similar to S-f2, a non-functional class I S haplotype identified in another self-compatible B. rapa cultivar, Yellow Sarson. Mutations of MLPK (M-locus protein kinase) and non-transcription of the male determinant, SCR, were observed in this cultivar, which is similar to the situation reported in Yellow Sarson. With respect to the female determinant, SRK, no transcript was detected in Yellow Sarson but two fragments were detected in Dahuangyoucai. One fragment was highly similar to SRK-f2, but the other fragment was different from the signal factors previously identified in the SI reaction. The results suggest that Dahuangyoucai and Yellow Sarson have the same origin and a similar mechanism of self-compatibility, but diverge after mutations in SRK, SCR and MLPK. Further studying the self-compatibility of Dahuangyoucai might identify novel factors involved in the SI signalling cascade and provide new insights into the mechanisms of SI in Brassicaceae.

scholarly journals Self-compatibility in Brassica napus is caused by independent mutations in S-locus genes

2007 ◽  
Vol 50 (3) ◽  
pp. 391-400 ◽  
Author(s):  
Shunsuke Okamoto ◽  
Masashi Odashima ◽  
Ryo Fujimoto ◽  
Yutaka Sato ◽  
Hiroyasu Kitashiba ◽  
...  
Keyword(s):  
Brassica Napus ◽  
S Locus ◽  
Self Compatibility

scholarly journals Genomic Organization of the S Locus: Identification and Characterization of Genes in SLG/SRK Region of S9 Haplotype of Brassica campestris (syn. rapa)

Genetics ◽  
1999 ◽  
Vol 153 (1) ◽  
pp. 391-400 ◽  
Author(s):  
Go Suzuki ◽  
Naoko Kai ◽  
Tamaki Hirose ◽  
Kiichi Fukui ◽  
Takeshi Nishio ◽  
...  
Keyword(s):  
Brassica Campestris ◽  
Protein A ◽  
S Locus ◽  
Cysteine Rich Protein ◽  
Pollen Coat ◽  
Genes Encoding ◽  
Gene Rich Region ◽  
Identification And Characterization ◽  
S Locus Glycoprotein

Abstract In Brassica, two self-incompatibility genes, encoding SLG (S locus glycoprotein) and SRK (S-receptor kinase), are located at the S locus and expressed in the stigma. Recent molecular analysis has revealed that the S locus is highly polymorphic and contains several genes, i.e., SLG, SRK, the as-yet-unidentified pollen S gene(s), and other linked genes. In the present study, we searched for expressed sequences in a 76-kb SLG/SRK region of the S9 haplotype of Brassica campestris (syn. rapa) and identified 10 genes in addition to the four previously identified (SLG9, SRK9, SAE1, and SLL2) in this haplotype. This gene density (1 gene/5.4 kb) suggests that the S locus is embedded in a gene-rich region of the genome. The average G + C content in this region is 32.6%. An En/Spm-type transposon-like element was found downstream of SLG9. Among the genes we identified that had not previously been found to be linked to the S locus were genes encoding a small cysteine-rich protein, a J-domain protein, and an antisilencing protein (ASF1) homologue. The small cysteine-rich protein was similar to a pollen coat protein, named PCP-A1, which had previously been shown to bind SLG.

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