Analysis of self-incompatibility interactions in 30 resynthesized Brassica napus lines. II. Expression of S-locus glycoproteins (SLGs)

1999 ◽  
Vol 99 (5) ◽  
pp. 885-890 ◽  
Author(s):  
M. Beschorner ◽  
V. Heberer ◽  
W. Odenbach
Keyword(s):  
Brassica Napus ◽  
Self Incompatibility ◽  
S Locus ◽  
Resynthesized Brassica Napus

Latent S alleles are widespread in cultivated self-compatible Brassica napus

Genome ◽  
2004 ◽  
Vol 47 (2) ◽  
pp. 257-265 ◽  
Author(s):  
U U Ekuere ◽  
I A.P Parkin ◽  
C Bowman ◽  
D Marshall ◽  
D J Lydiate
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Self Incompatibility ◽  
S Locus ◽  
Winter Oilseed Rape ◽  
A Genome ◽  
C Genome ◽  
S Alleles ◽  
Crop Types ◽  
S Allele

The genetic control of self-incompatibility in Brassica napus was investigated using crosses between resynthesized lines of B. napus and cultivars of oilseed rape. These crosses introduced eight C-genome S alleles from Brassica oleracea (S16, S22, S23, S25, S29, S35, S60, and S63) and one A-genome S allele from Brassica rapa (SRM29) into winter oilseed rape. The inheritance of S alleles was monitored using genetic markers and S phenotypes were determined in the F1, F2, first backcross (B1), and testcross (T1) generations. Two different F1 hybrids were used to develop populations of doubled haploid lines that were subjected to genetic mapping and scored for S phenotype. These investigations identified a latent S allele in at least two oilseed rape cultivars and indicated that the S phenotype of these latent alleles was masked by a suppressor system common to oilseed rape. These latent S alleles may be widespread in oilseed rape varieties and are possibly associated with the highly conserved C-genome S locus of these crop types. Segregation for S phenotype in subpopulations uniform for S genotype suggests the existence of suppressor loci that influenced the expression of the S phenotype. These suppressor loci were not linked to the S loci and possessed suppressing alleles in oilseed rape and non-suppressing alleles in the diploid parents of resynthesized B. napus lines.Key words: self-incompatibility, B. oleracea, B. rapa, S locus, suppression.

scholarly journals Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2186
Author(s):  
Zhiquan Liu ◽  
Bing Li ◽  
Yong Yang ◽  
Changbin Gao ◽  
Bin Yi ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Locus ◽  
Recognition System ◽  
Inbred Lines ◽  
The Self ◽  
Self Incompatibility ◽  
S Locus ◽  
S Haplotype ◽  
Self Compatibility

Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus.

Conservation of S-locus for self-incompatibility in Brassica napus (L.) and Brassica oleracea (L.)

1997 ◽  
Vol 95 (1-2) ◽  
pp. 73-82 ◽  
Author(s):  
W. Y. Cheung ◽  
G. Champagne ◽  
N. Hubert ◽  
L. Tulsieram ◽  
D. Charne ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Self Incompatibility ◽  
S Locus ◽  
Brassica Napus L ◽  
Brassica Oleracea L

scholarly journals Molecular characterization of the S locus in two self-incompatible Brassica napus lines.

1996 ◽  
Vol 8 (12) ◽  
pp. 2369-2380 ◽  
Author(s):  
K Yu ◽  
U Schafer ◽  
T L Glavin ◽  
D R Goring ◽  
S J Rothstein
Keyword(s):  
Brassica Napus ◽  
Molecular Characterization ◽  
S Locus

Sequence and Structural Diversity of the S Locus Genes From Different Lines With the Same Self-Recognition Specificities in Brassica oleracea

Genetics ◽  
2000 ◽  
Vol 154 (1) ◽  
pp. 413-420 ◽  
Author(s):  
Makoto Kusaba ◽  
Masanori Matsushita ◽  
Keiichi Okazaki ◽  
Yoko Satta ◽  
Takeshi Nishio
Keyword(s):  
Structural Diversity ◽  
Receptor Protein ◽  
Self Incompatibility ◽  
S Locus ◽  
High Similarity ◽  
Putative Receptor ◽  
Recognition Specificity ◽  
Polymorphic Genes ◽  
Self Recognition ◽  
Self Fertilization

Abstract Self-incompatibility (SI) is a mechanism for preventing self-fertilization in flowering plants. In Brassica, it is controlled by a single multi-allelic locus, S, and it is believed that two highly polymorphic genes in the S locus, SLG and SRK, play central roles in self-recognition in stigmas. SRK is a putative receptor protein kinase, whose extracellular domain exhibits high similarity to SLG. We analyzed two pairs of lines showing cross-incompatibility (S2 and S2-b; S13 and S13-b). In S2 and S2-b, SRKs were more highly conserved than SLGs. This was also the case with S13 and S13-b. This suggests that the SRKs of different lines must be conserved for the lines to have the same self-recognition specificity. In particular, SLG2-b showed only 88.5% identity to SLG2, which is comparable to that between the SLGs of different S haplotypes, while SRK2-b showed 97.3% identity to SRK2 in the S domain. These findings suggest that the SLGs in these S haplotypes are not important for self-recognition in SI.

Visualization of the S-locus region in Ipomoea trifida: toward positional cloning of self-incompatibility genes

2004 ◽  
Vol 12 (5) ◽  
pp. 475-481 ◽  
Author(s):  
Go Suzuki ◽  
Saiko Tanaka ◽  
Maki Yamamoto ◽  
Rubens Norio Tomita ◽  
Yasuo Kowyama ◽  
...  
Keyword(s):  
Positional Cloning ◽  
Self Incompatibility ◽  
S Locus ◽  
Ipomoea Trifida
Sign in / Sign up

Export Citation Format

Share Document