Induction of microspore embryogenesis in Brassica napus L. is accompanied by specific changes in protein synthesis

The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) applied at high concentrations for a short time was investigated as a novel stress for induction of microspore embryogenesis for the first time. Brassica napus L. cvs. Topas and Hyola 420 were used as model plants for testing this hypothesis. Microspores were subjected to 2,4-D at 4 concentrations (15, 25, 35 and 45 mg/l) for 15–45 min while the classical heat shock was used as the control treatment. Among 2,4-D treatments in Topas, the highest yield of torpedo-stage embryos was achieved at 15 mg/l 2,4-D for 30 min while more normal plantlets were produced when 2,4-D (25 mg/l for 30 min) was applied to the microspores. In Hyola 420 the results showed a lower number of embryos and normal plantlets at all concentrations of 2,4-D. Although Hyola 420 was almost equally embryogenic as Topas after heat shock treatment, large differences between genotypes (concerning embryogenic response) occurred after 2,4-D treatment. However, the mean number of embryos and regenerants was higher in heat shock as compared to 2,4-D induced stress (one magnitude of order). According to the results obtained, 2,4-D can be introduced as a new stress for induction of embryogenesis in microspores similarly like in zygotic and somatic cells. This novel stress is very important for plant species whose microspores are extremely sensitive to classical stresses.

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Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.)

Plant Growth Regulation ◽

10.1023/b:grow.0000017488.29181.fa ◽

2004 ◽

Vol 42 (2) ◽

pp. 137-143 ◽

Cited By ~ 53

Author(s):

H.H. Gu ◽

P. Hagberg ◽

W.J. Zhou

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Microspore Embryogenesis ◽

Cold Pretreatment ◽

Brassica Napus L

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Effects of ascorbic acid, alpha-tocopherol, and glutathione on microspore embryogenesis in Brassica napus L.

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-013-9579-8 ◽

2013 ◽

Vol 50 (1) ◽

pp. 26-35 ◽

Cited By ~ 13

Author(s):

Maryam Hoseini ◽

Mortaza Ghadimzadeh ◽

Behzad Ahmadi ◽

Jaime A. Teixeira da Silva

Keyword(s):

Ascorbic Acid ◽

Brassica Napus ◽

Microspore Embryogenesis ◽

Alpha Tocopherol ◽

Brassica Napus L

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Characterization of polarity development through 2- and 3-D imaging during the initial phase of microspore embryogenesis in Brassica napus L

PROTOPLASMA ◽

10.1007/s00709-013-0530-y ◽

2013 ◽

Vol 251 (1) ◽

pp. 103-113 ◽

Cited By ~ 7

Author(s):

Ewa Dubas ◽

Jan Custers ◽

Henk Kieft ◽

Maria Wędzony ◽

André A. M. van Lammeren

Keyword(s):

Brassica Napus ◽

Initial Phase ◽

Microspore Embryogenesis ◽

Brassica Napus L

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INCORPORATION OF 14C INTO TWO PROTEINS FROM RAPESEED (BRASSICA NAPUS L.)

Canadian Journal of Biochemistry ◽

10.1139/o66-117 ◽

1966 ◽

Vol 44 (7) ◽

pp. 997-1004 ◽

Cited By ~ 7

Author(s):

A. J. Finlayson

Keyword(s):

Amino Acids ◽

Protein Synthesis ◽

Brassica Napus ◽

Sodium Acetate ◽

Water Extract ◽

Seed Meal ◽

Brassica Napus L ◽

Specific Activities ◽

Considerable Period

Four groups of five rapeseed plants (Brassica napus L.) were injected with sodium acetate-2-14C at intervals between flowering and maturity. Two 14C-labeled proteins were isolated from a water extract of the oil-free seed meal. The specific activities of the two proteins and of some amino acids isolated from them suggested rate differences in protein synthesis, or, alternatively, conversion of one protein into another. The results also showed that synthesis of these proteins occurs over a considerable period in the plant's development.

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