Induction of microspore embryogenesis in Brassica napus L. is accompanied by specific changes in protein synthesis

Planta ◽  
1994 ◽  
Vol 195 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Jan H. G. Cordewener ◽  
Ronald Busink ◽  
Jan A. Traas ◽  
Jan B. M. Custers ◽  
Hans J. M. Dons ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Brassica Napus ◽  
Microspore Embryogenesis ◽  
Brassica Napus L

scholarly journals  Effect of 2,4-D as a novel inducer of embryogenesis in microspores of Brassica napus L.

2011 ◽  
Vol 47 (No. 3) ◽  
pp. 114-122 ◽  
Author(s):  
S.H. Ardebili ◽  
M.E. Shariatpanahi ◽  
R. Amiri ◽  
M. Emamifar ◽  
M. Oroojloo ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Heat Shock ◽  
Microspore Embryogenesis ◽  
Control Treatment ◽  
Dichlorophenoxyacetic Acid ◽  
Brassica Napus L ◽  
High Concentrations ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Short Time ◽  
First Time

The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) applied at high concentrations for a short time was investigated as a novel stress for induction of microspore embryogenesis for the first time. Brassica napus L. cvs. Topas and Hyola 420 were used as model plants for testing this hypothesis. Microspores were subjected to 2,4-D at 4 concentrations (15, 25, 35 and 45 mg/l) for 15–45 min while the classical heat shock was used as the control treatment. Among 2,4-D treatments in Topas, the highest yield of torpedo-stage embryos was achieved at 15 mg/l 2,4-D for 30 min while more normal plantlets were produced when 2,4-D (25 mg/l for 30 min) was applied to the microspores. In Hyola 420 the results showed a lower number of embryos and normal plantlets at all concentrations of 2,4-D. Although Hyola 420 was almost equally embryogenic as Topas after heat shock treatment, large differences between genotypes (concerning embryogenic response) occurred after 2,4-D treatment. However, the mean number of embryos and regenerants was higher in heat shock as compared to 2,4-D induced stress (one magnitude of order). According to the results obtained, 2,4-D can be introduced as a new stress for induction of embryogenesis in microspores similarly like in zygotic and somatic cells. This novel stress is very important for plant species whose microspores are extremely sensitive to classical stresses.

Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.)

2004 ◽  
Vol 42 (2) ◽  
pp. 137-143 ◽  
Author(s):  
H.H. Gu ◽  
P. Hagberg ◽  
W.J. Zhou
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Microspore Embryogenesis ◽  
Cold Pretreatment ◽  
Brassica Napus L

Effects of ascorbic acid, alpha-tocopherol, and glutathione on microspore embryogenesis in Brassica napus L.

2013 ◽  
Vol 50 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Maryam Hoseini ◽  
Mortaza Ghadimzadeh ◽  
Behzad Ahmadi ◽  
Jaime A. Teixeira da Silva
Keyword(s):  
Ascorbic Acid ◽  
Brassica Napus ◽  
Microspore Embryogenesis ◽  
Alpha Tocopherol ◽  
Brassica Napus L

scholarly journals Characterization of polarity development through 2- and 3-D imaging during the initial phase of microspore embryogenesis in Brassica napus L

PROTOPLASMA ◽  
2013 ◽  
Vol 251 (1) ◽  
pp. 103-113 ◽  
Author(s):  
Ewa Dubas ◽  
Jan Custers ◽  
Henk Kieft ◽  
Maria Wędzony ◽  
André A. M. van Lammeren
Keyword(s):  
Brassica Napus ◽  
Initial Phase ◽  
Microspore Embryogenesis ◽  
Brassica Napus L

INCORPORATION OF 14C INTO TWO PROTEINS FROM RAPESEED (BRASSICA NAPUS L.)

1966 ◽  
Vol 44 (7) ◽  
pp. 997-1004 ◽  
Author(s):  
A. J. Finlayson
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Brassica Napus ◽  
Sodium Acetate ◽  
Water Extract ◽  
Seed Meal ◽  
Brassica Napus L ◽  
Specific Activities ◽  
Considerable Period

Four groups of five rapeseed plants (Brassica napus L.) were injected with sodium acetate-2-14C at intervals between flowering and maturity. Two 14C-labeled proteins were isolated from a water extract of the oil-free seed meal. The specific activities of the two proteins and of some amino acids isolated from them suggested rate differences in protein synthesis, or, alternatively, conversion of one protein into another. The results also showed that synthesis of these proteins occurs over a considerable period in the plant's development.

Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

2015 ◽  
Vol 35 (1) ◽  
pp. 185-193 ◽  
Author(s):  
Behzad Ahmadi ◽  
Farhad Masoomi-Aladizgeh ◽  
Mehran E. Shariatpanahi ◽  
Pejman Azadi ◽  
Mehdi Keshavarz-Alizadeh
Keyword(s):  
Brassica Napus ◽  
Plant Regeneration ◽  
Molecular Characterization ◽  
Expression Analysis ◽  
Microspore Embryogenesis ◽  
Brassica Napus L

Improved microspore embryogenesis induction and plantlet regeneration using putrescine, cefotaxime and vancomycin in Brassica napus L.

2014 ◽  
Vol 118 (3) ◽  
pp. 497-505 ◽  
Author(s):  
Behzad Ahmadi ◽  
Mehran E. Shariatpanahi ◽  
Mehdi Aghapour Ojaghkandi ◽  
Ali Akbar Heydari
Keyword(s):  
Brassica Napus ◽  
Microspore Embryogenesis ◽  
Plantlet Regeneration ◽  
Brassica Napus L

Effect of boron on microspore embryogenesis and direct embryo to plant conversion in Brassica napus (L.)

2017 ◽  
Vol 130 (2) ◽  
pp. 443-447 ◽  
Author(s):  
Pitchayapa Mahasuk ◽  
Annika Stina Kullik ◽  
Mohammed Cassim Iqbal ◽  
Christian Möllers
Keyword(s):  
Brassica Napus ◽  
Microspore Embryogenesis ◽  
Brassica Napus L ◽  
Plant Conversion

scholarly journals A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L.

2011 ◽  
Vol 168 (8) ◽  
pp. 746-757 ◽  
Author(s):  
J.M. Seguí-Simarro ◽  
P. Corral-Martínez ◽  
E. Corredor ◽  
I. Raska ◽  
P.S. Testillano ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Microspore Embryogenesis ◽  
Brassica Napus L ◽  
Developmental Program

scholarly journals Mitogen-activated protein kinases are developmentally regulated during stress-induced microspore embryogenesis in Brassica napus L

2005 ◽  
Vol 123 (4-5) ◽  
pp. 541-551 ◽  
Author(s):  
José M. Seguí-Simarro ◽  
Pilar S. Testillano ◽  
Stefan Jouannic ◽  
Yves Henry ◽  
Maria C. Risueño
Keyword(s):  
Brassica Napus ◽  
Protein Kinases ◽  
Microspore Embryogenesis ◽  
Brassica Napus L ◽  
Developmentally Regulated ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein
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