Isolation on � VERO � cells of a Spotted Fever Group Rickettsia � Mortilli strain � from Rhipicephalus sanguineus

1986 ◽  
Vol 2 (3) ◽  
Author(s):  
G. Tringali ◽  
V. Intonazzo ◽  
A.M. Perna ◽  
S. Mansueto ◽  
D.H. Walker
Keyword(s):  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
Vero Cells ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsia

scholarly journals Comparative growth of spotted fever group Rickettsia spp. strains in Vero cells

2016 ◽  
Vol 111 (8) ◽  
pp. 528-531
Author(s):  
Arannadia Barbosa Silva ◽  
Myrian Morato Duarte ◽  
Vinicius Figueiredo Vizzoni ◽  
Ana Íris de Lima Duré ◽  
Diego Montenegro Lopéz ◽  
...  
Keyword(s):  
Spotted Fever ◽  
Vero Cells ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsia

scholarly journals Isolation and Identification of Rickettsia massiliae from Rhipicephalus sanguineus Ticks Collected in Arizona

2006 ◽  
Vol 72 (8) ◽  
pp. 5569-5577 ◽  
Author(s):  
Marina E. Eremeeva ◽  
Elizabeth A. Bosserman ◽  
Linda J. Demma ◽  
Maria L. Zambrano ◽  
Dianna M. Blau ◽  
...  
Keyword(s):  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
Vero Cells ◽  
The United States ◽  
Spotted Fever Group ◽  
Pcr Assay ◽  
Isolation And Identification ◽  
Cytotoxic Effects ◽  
Rickettsia Massiliae ◽  
Genotypic Characteristics

ABSTRACT Twenty Rhipicephalus sanguineus ticks collected in eastern Arizona were tested by PCR assay to establish their infection rate with spotted fever group rickettsiae. With a nested PCR assay which detects a fragment of the Rickettsia genus-specific 17-kDa antigen gene (htrA), five ticks (25%) were found to contain rickettsial DNA. One rickettsial isolate was obtained from these ticks by inoculating a suspension of a triturated tick into monolayers of Vero E6 monkey kidney cells and XTC-2 clawed toad cells, and its cell culture and genotypic characteristics were determined. Fragments of the 16S rRNA, GltA, rOmpA, rOmpB, and Sca4 genes had 100%, 100%, 99%, 99%, and 99%, respectively, nucleotide similarity to Rickettsia massiliae strain Bar29, previously isolated from R. sanguineus in Catalonia, Spain (L. Beati et al., J. Clin. Microbiol. 34:2688-2694, 1996). The new isolate, AZT80, does not elicit cytotoxic effects in Vero cells and causes a persistent infection in XTC-2 cells. The AZT80 strain is susceptible to doxycycline but resistant to rifampin and erythromycin. Whether R. massiliae AZT80 is pathogenic or infectious for dogs and humans or can cause seroconversion to spotted fever group antigens in the United States is unknown.

scholarly journals Isolation of an agent of the spotted fever group rickettsia from tick eggs in Madrid, Spain

1992 ◽  
Vol 108 (3) ◽  
pp. 555-557 ◽  
Author(s):  
C. Herrero ◽  
C. Pelaz ◽  
C. Martín-Bourgon
Keyword(s):  
Human Serum ◽  
Indirect Immunofluorescence ◽  
Spotted Fever ◽  
Vero Cells ◽  
Rickettsia Conorii ◽  
Spotted Fever Group ◽  
Suitable Material ◽  
Rural Villages ◽  
Spotted Fever Group Rickettsia

SUMMARYTicks recovered from dogs in rural villages around Madrid (Spain) were processed to isolate rickettsiae. One sample containing mixtures of ticks and four containing eggs, in which rickettsiae had been detected by indirect immunofluorescence with a human serum highly reactive toRickettsia conorii, were decontaminated, homogenized and inoculated onto Vero cells. Two egg samples yielded a cytopathic agent that reacted positively by immunofluorescence. One sample (14H) was successfully subcultured and identified as a member of the spotted fever group rickettsia. Tick eggs provide suitable material for isolation of rickettsia.

scholarly journals Genotypic evaluation of rickettsial isolates recovered from various species of ticks in Portugal

1995 ◽  
Vol 114 (1) ◽  
pp. 169-178 ◽  
Author(s):  
F. Bacellar ◽  
R. L. Regnery ◽  
M. S. Núncio ◽  
A. R. Filipe
Keyword(s):  
Restriction Endonuclease ◽  
Dna Sequences ◽  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
Spotted Fever Group ◽  
Potential Vector ◽  
Genotypic Analysis ◽  
Restriction Endonuclease Cleavage Site ◽  
Spotted Fever Group Rickettsia ◽  
First Time

SUMMARYTwelve rickettsial isolates, fromRhipicephalus sanguineus, R. turanicus, Dermacentor marginatusandHyalomma marginatus, were subjected to genotypic analysis. Amplification of specific DNA sequences, restriction endonuclease digestion of amplified DNA products, and gel electrophoresis were used to identify specific DNA fragment-banding patterns. Five patterns were resolved. Four were homologous with those of previously described rickettsial genotypes,R. conorii, R. slovaca, R. rhipicephaliandR. massiliae. The fifth pattern differed by only a single altered restriction endonuclease cleavage site. For the first time in Portugal a widely distributed spectrum of spotted fever group rickettsia was found among potential vector species stressing the need to determine their potential for human and domestic animals infection.

scholarly journals Rhipicephalus sanguineus: vector of a new spotted fever group rickettsia in the United States.

1975 ◽  
Vol 12 (1) ◽  
pp. 205-210 ◽  
Author(s):  
W Burgdorfer ◽  
D J Sexton ◽  
R K Gerloff ◽  
R L Anacker ◽  
R N Philip ◽  
...  
Keyword(s):  
United States ◽  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
The United States ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsia

scholarly journals Evidence of spotted fever group rickettsiae in state of Rio de Janeiro, Brazil

2002 ◽  
Vol 44 (3) ◽  
pp. 155-158 ◽  
Author(s):  
Tatiana ROZENTAL ◽  
Maria Cristina BUSTAMANTE ◽  
Marinete AMORIM ◽  
Nicolau Maués SERRA-FREIRE ◽  
Elba Regina Sampaio de LEMOS
Keyword(s):  
Rio De Janeiro ◽  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
Direct Immunofluorescence ◽  
Spotted Fever Group ◽  
Limited Information ◽  
Amblyomma Cajennense ◽  
Preliminary Results ◽  
Spotted Fever Group Rickettsiae ◽  
Spotted Fever Group Rickettsia

Ticks were obtained from dogs from February to September of 1999 at weekly intervals, in the County of Piraí, State of Rio de Janeiro. Four hundred seventy four ixodids were taxonomically identified, 103 Amblyomma cajennense, seven Amblyomma ovale, 209 Rhipicephalus sanguineus, and 155 Amblyomma sp. An hemolymph test associated with Giemsa's stain revealed two specimens in 163 ticks tested (R. sanguineus and Amblyomma sp), containing rickettsia-like organisms. Direct immunofluorescence verified the presence of spotted fever group rickettsia in one specimen of R. sanguineus. Considering the limited information on rickettsiosis in Brazil, principally in relation to the vectors involved in perpetuating it in foci, these preliminary results give us an idea on the importance of infection in ticks, allowing to expand our knowledge on this zoonosis.

scholarly journals Spotted fever group Rickettsia in brown dog ticks Rhipicephalus sanguineus in southwestern Spain

2008 ◽  
Vol 103 (1) ◽  
pp. 119-122 ◽  
Author(s):  
F. J. Márquez ◽  
J. J. Rodríguez-Liébana ◽  
R. C. Soriguer ◽  
M. A. Muniaín ◽  
M. Bernabeu-Wittel ◽  
...  
Keyword(s):  
Spotted Fever ◽  
Rhipicephalus Sanguineus ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsia
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