Pharmacognostical Studies on Leaves of Madhuca longifolia (koen) Macbr

Madhuca longifolia (koen) macbr is an ever-green tree belonging to family sapotaceae commonly known as mahua. The leaf is used by the indigenous people of Australia in curing bleeding, gums, expectorant, cushing`s disease, wound healing activity and various ailments. The present study comprises macroscopy, microscopy, histochemistry, physicochemical parameters, phytochemical studies and florescence analysis. Pharmacognosy is the initial step for determining the status of organ of plant considered as a crude medicine; hence the current study was done, TLC of flavonoid as a chemical constituents present in the drug for establishing the biomarker compound. These studies will help in future for establishing the pharmacopoeial drug standards.

Cytotoxicity Assessment of Heritiera Littoralis (Aiton), Madhuca Longifolia (König) Macbr., Nerium Indicum Mill. And Sapium Indicum (Willd.) Leaves on Artemia Salina (L.)

Petroleum ether, CHCl3 and CH3OH extracts of leaves of Heritiera littoralis (Aiton), Madhuca longifolia (König) Macbr., Nerium indicum Mill. and Sapium indicum (Willd.) were subjected to assess cytotoxicity against Artemia salina (L.) nauplii. The petroleum ether extract of H. littoralis leaves showed LC50 values 273.77, 97.27, 51.60, 37.12, 14.60 and 12.59 ppm after 12, 18, 24, 30, 36 and 42 h; the CHCl3 extract showed LC50 values 733.25, 105.51, 40.72 and 18.20 ppm after 6, 12, 18 and 24 h whereas CH3OH extract showed 73.05, 30.62, 24.56, 20.85, 16.21 and 6.71 ppm after 6, 12, 18, 24, 30 and 36 h of exposure respectively. The petroleum ether extract of M. longifolia leaves possess LC50 values 259.35, 115.17, 56.84 and 8.73 ppm after 12, 18, 24 and 30 h; the CHCl3 extract possess LC50 values 585.43, 205.86, 112.74, 75.62, 52.84and 47.34 ppm after 12, 18, 24, 30, 36 and 42 h but CH3OH extract possess LC50 values 185.87, 60.70, 30.11 and 15.39 ppm after 12, 18, 24 and 30 h of exposure respectively. The petroleum ether extract of N. Indicum leaves recorded LC50 values 249.82, 146.07, 80.23, 54.21 and 40.19 ppm after 18, 24, 30, 36 and 42 h; the CHCl3 extract gave LC50 values 36.13, 21.72, 19.03, 16.81 and 16.34 ppm after 12, 18, 24, 30 and 36 h but CH3OH extract recorded LC50 values 394.90, 129.69, 81.50, 73.10 and 37.51 ppm after 18, 24, 30, 36 and 42 h of exposure respectively. Similarly, the petroleum ether extract of S. indicum leaves showed LC50 values 24.79, 13.18 and 4.61 ppm after 12, 18 and 24 h; the CHCl3 extract were 50.45, 42.64, 21.20 and 14.93 ppm after 18, 24, 30 and 36 h of exposure and the CH3OH extract showed LC50 values 306.37, 217.18, 149.38, 73.52, 54.45 and 22.91 ppm after 12, 18, 24, 30, 36 and 42 h of exposure respectively. The intensity of efficacy of the extracts could be arranged in the following descending order of S. indicum (petroleum etroleum ether extract) >H. littoralis (CH3OH extract) >M. longifolia (petroleum ether extract) >N. indicum (CHCl3 extract). J. Bio-Sci. 29(2): 139-149, 2021 (December)

Apoptotic mechanisms of myricitrin isolated from Madhuca longifolia leaves in HL-60 leukemia cells

Background: Myricitrin, a naturally occurring flavonoid in Madhuca longifolia, possesses several medicinal properties. Even though our earlier work revealed its role against the proliferation of acute myelogenous leukemia cells (HL-60), its molecular mechanisms have not yet been revealed. The current study aims to explore the molecular mechanisms of myricitrin (isolated from an ethnomedicinal drug Madhuca longifolia) to induce apoptosis in HL-60 cells. Methods and Results: Treatment with IC-50 dose of myricitrin (353 µM) caused cellular shrinkage and cell wall damage in HL-60 cells compared to untreated control cells. Myricitrin treatment reduced the mitochondrial membrane potential (22.95%), increased DNA fragmentation (90.4%), inhibited the cell survival proteins (RAS, B-RAF, & BCL-2) and also induced pro-apoptotic proteins (p38, pro-caspase-3, pro-caspase-9 and caspase-3) in the HL-60 cells. Conclusions: The present study provides scientific evidence for the apoptosis caused by myricitrin in HL-60 leukemia cells. Hence, the phytochemical myricitrin could be considered as a potential candidate to develop an anticancer drug after checking its efficacy through suitable pre-clinical and clinical studies.

Comprehensive Profile Of Madhuca Longifolia In Pharma And Food Industry

: Madhuca longifolia (J.Koenig) J.F.Macbr. belongs to the family Sapotaceae, also commonly known as mahua or butternut tree. Each part of the plant is known for its unique medicinal properties. This review aims to enhance the existence of application and extend the therapeutic potential of Madhuca species in Pharmaceuticals, cosmeceuticals, agriculture and the food industry. Madhuca longifolia and all its products are widely used as a traditional and herbal medicine system. Even though they are used traditionally by a population of local areas, the potential of therapeutic existence is still uncovered. This systematic review is an exhaustive compilation of detailed data on phytochemistry, wide ethnopharmacological uses, medicinal properties and commercial application of mahua in different sectors. With the potential of spasmogenic, oxytocic, uterotonic, anti-bacterial, anti implantation, anti-tumor, anti-progestational, antiestrogenic activity against menorrhagia and anti-cancer, and many more. M. longifolia is also used in stomach ache, snake bite, scorpion bite, bone fractures, treatment of piles, joint pains, increase lactation, etc. It can contribute towards the development of the pharmaceutical sector and food industry too. Positively it will provide a path for future research for the development of herbal drugs and other products for agriculture and the food industry.

Comparative Evaluation of Madhuca Longifolia and Jatropha Curcas Extracted Biodiesel.

In order to fulfil the growing need to replace fossil fuels, investigations exploring the production of biodiesel from agricultural biomass have gained attention. The purpose behind this study is to examine the efficiency of biodiesel extracted from Madhuca longifolia and Jatropha curcas by means of pre-treatment followed by two step acid-base homogeneous catalyst method. The Madhuca and Jatropha biodiesels were blended with diesel at different percentages. Fourier Transform Infrared Spectroscopy (FTIR) showed a strong presence of fatty acid profile, and triglyceride ester linkage at 1744 cm− 1. UV–Vis spectra confirmed the presence of conjugated dienes in the extracted biodiesels. UV absorbance at 320 nm decreases linearly with blend percentage. 1HNMR and 13C NMR confirmed the presence of methyl ester moiety at 3.6 ppm and methoxy carbon at 51.2 ppm in biodiesel distinguishing it from diesel. Raman spectra exhibited C = O stretching at 1725 cm− 1 indicating conversion of Madhuca and Jatropha oil into biodiesel. The engine performance and emission tests of these biodiesels were evaluated. In the engine performance tests, the variations of brake specific fuel consumption (BSFC), exhaust gas temperature (EGT), and brake thermal efficiency (BTE) versus brake power were studied. The emission tests of different blends were done in terms of carbon mono-oxide, nitrous oxide, and unburnt hydrocarbon (UHC). The Jatropha biodiesel exhibited less mean BSFC, EGT and emitted less CO and UHC than Madhuca biodiesel. The average decrease in BTE was more in Jatropha biodiesel than Madhuca biodiesel.