A novel promoter, derived from the isocitrate lyase gene of Candida tropicalis, inducible with acetate in Saccharomyces cerevisiae

1995 ◽  
Vol 43 (3) ◽  
pp. 489-492 ◽  
Author(s):  
K. Umemura ◽  
H. Atomi ◽  
T. Kanai ◽  
Y. Teranishi ◽  
M. Ueda ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Tropicalis ◽  
Isocitrate Lyase

Localization of Candida tropicalis peroxisomal isocitrate lyase highly expressed in peroxisome-proliferated Saccharomyces cerevisiae

1992 ◽  
Vol 74 (6) ◽  
pp. 368-371 ◽  
Author(s):  
Yasushi Kamada ◽  
Mitsuyoshi Ueda ◽  
Haruyuki Atomi ◽  
Keinosuke Oda ◽  
Tatsuo Kurihara ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Tropicalis ◽  
Isocitrate Lyase

Subcellular Destination of Mutant Peroxisomal Isocitrate Lyase Polypeptides of Candida tropicalis in Saccharomyces cerevisiae

1996 ◽  
Vol 45 (6) ◽  
pp. 491-497 ◽  
Author(s):  
N. Kamasawa ◽  
T. Yoshida ◽  
M. Kasahara ◽  
Y. Kamada ◽  
W. Zou ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Tropicalis ◽  
Isocitrate Lyase

A novel promoter, derived from the isocitrate lyase gene of Candida tropicalis, inducible with acetate in Saccharomyces cerevisiae

1995 ◽  
Vol 43 (3) ◽  
pp. 489-492 ◽  
Author(s):  
K. Umemura ◽  
H. Atomi ◽  
T. Kanai ◽  
Y. Teranishi ◽  
M. Ueda ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Tropicalis ◽  
Isocitrate Lyase

scholarly journals The Identification and Characterization of ADR6, a Gene Required for Sporulation and for Expression of the Alcohol Dehydrogenase II Isozyme From Saccharomyces cerevisiae

Genetics ◽  
1987 ◽  
Vol 116 (4) ◽  
pp. 523-530
Author(s):  
Aileen K W Taguchi ◽  
Elton T Young
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alcohol Dehydrogenase ◽  
Isocitrate Lyase ◽  
Carbon Sources ◽  
Recessive Mutation ◽  
Yeast Saccharomyces Cerevisiae ◽  
Upstream Activating Sequence ◽  
Adh Activity ◽  
Identification And Characterization

ABSTRACT The alcohol dehydrogenase II isozyme (enzyme, ADHII; structural gene, ADH2) of the yeast, Saccharomyces cerevisiae, is under stringent carbon catabolite control. This cytoplasmic isozyme exhibits negligible activity during growth in media containing fermentable carbon sources such as glucose and is maximal during growth on nonfermentable carbon sources. A recessive mutation, adr6-1, and possibly two other alleles at this locus, were selected for their ability to decrease Ty-activated ADH2-6 c expression. The adr6-1 mutation led to decreased ADHII activity in both ADH2-6c and ADH2+ strains, and to decreased levels of ADH2 mRNA. Ty transcription and the expression of two other carbon catabolite regulated enzymes, isocitrate lyase and malate dehydrogenase, were unaffected by the adr6-1 mutation. adr6-1/adr6-1strains were defective for sporulation, indicating that adr6 mutations may have pleiotropic effects. The sporulation defect was not a consequence of decreased ADH activity. Since the ADH2-6c mutation is due to insertion of a 5.6-kb Ty element at the TATAA box, it appears that the ADR6+-dependent ADHII activity required ADH2 sequences 3′ to or including the TATAA box. The ADH2 upstream activating sequence (UAS) was probably not required. The ADR6 locus was unlinked to the ADR1 gene which encodes another trans-acting element required for ADH2 expression.

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