A novel heterologous gene expression system in Saccharomyces cerevisiae using the isocitrate lyase gene promoter from Candida tropicalis

1996 ◽  
Vol 44 (6) ◽  
pp. 759-765 ◽  
Author(s):  
T. Kanai ◽  
H. Atomi ◽  
K. Umemura ◽  
H. Ueno ◽  
Y. Teranishi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Candida Tropicalis ◽  
Isocitrate Lyase ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Gene Promoter ◽  
Heterologous Gene ◽  
Gene Expression System

Creation of a heterologous gene expression system on the basis of Aspergillus awamori recombinant strain

2011 ◽  
Vol 47 (3) ◽  
pp. 279-287 ◽  
Author(s):  
A. M. Rozhkova ◽  
A. S. Sereda ◽  
N. V. Tsurikova ◽  
A. K. Nurtaeva ◽  
M. V. Semenova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Recombinant Strain ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Heterologous Gene ◽  
Aspergillus Awamori ◽  
Gene Expression System

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

Production of foreign proteins in the yeast Pichia pastoris

1995 ◽  
Vol 73 (S1) ◽  
pp. 891-897 ◽  
Author(s):  
James M. Cregg ◽  
David R. Higgins
Keyword(s):  
Gene Expression ◽  
Pichia Pastoris ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Methylotrophic Yeast ◽  
Culture Broth ◽  
Heterologous Gene ◽  
Foreign Gene ◽  
Heterologous Proteins ◽  
Yeast Pichia Pastoris

The methanol-utilizing yeast Pichia pastoris has been developed as a host system for the production of heterologous proteins of commercial interest. An industrial yeast selected for efficient growth on methanol for biomass generation, P. pastoris is readily grown on defined medium in continuous culture at high volume and density. A unique feature of the expression system is the promoter employed to drive heterologous gene expression, which is derived from the methanol-regulated alcohol oxidase I gene (AOX1) of P. pastoris, one of the most efficient and tightly regulated promoters known. The strength of the AOX1 promoter results in high expression levels in strains harboring only a single integrated copy of a foreign-gene expression cassette. Levels may often be further enhanced through the integration of multiple cassette copies into the P. pastoris genome and strategies to construct and select multicopy cassette strains have been devised. The system is particularly attractive for the secretion of foreign-gene products. Because P. pastoris endogenous protein secretion levels are low, foreign secreted proteins often appear to be virtually the only proteins in the culture broth, a major advantage in processing and purification. Key words: heterologous gene expression, methylotrophic yeast, Pichia pastoris, secretion, glycosylation.

High-level heterologous gene expression in Saccharomyces cerevisiae from a stable 2μm plasmid system

Gene ◽  
1993 ◽  
Vol 132 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Dale L. Ludwig ◽  
Simone Ugolini ◽  
Carlo V. Bruschi
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
High Level
Sign in / Sign up

Export Citation Format

Share Document