Mapping of a Magnaporthe grisea locus affecting rice (Oryza sativa) cultivar specificity

1994 ◽  
Vol 88 (8) ◽  
pp. 901-908 ◽  
Author(s):  
J. R. Smith ◽  
S. A. Leong
Keyword(s):  
Oryza Sativa ◽  
Magnaporthe Grisea ◽  
Cultivar Specificity

scholarly journals Inter-relação sexual de Magnaporthe grisea do trigo e de outros hospedeiros

2001 ◽  
Vol 26 (1) ◽  
pp. 21-26 ◽  
Author(s):  
ANA C. BRUNO ◽  
ALFREDO S. URASHIMA
Keyword(s):  
Triticum Aestivum ◽  
Oryza Sativa ◽  
Mating Type ◽  
Magnaporthe Grisea

Foram identificados isolados de Magnaporthe grisea do Brasil a serem utilizados como parentais em estudos de determinação do tipo compatível ("mating type") do fungo. A escolha dos melhores parentais baseou-se naqueles isolados que determinaram o tipo compatível de maior número de isolados de M. grisea do trigo (Triticum aestivum). A inter-relação sexual da brusone do trigo com a brusone de diversos hospedeiros foi avaliada de acordo com os seguintes parâmetros: determinação de tipo compatível (MAT1-1 e MAT1-2), sexualidade (hermafrodita, fêmea e macho), fertilidade (número de peritécios) e produção de órgãos sexuais (peritécio, ascas e ascósporos). Os parentais Bp3a e Br118.2D possibilitaram a identificação do tipo compatível de maior porcentagem dos isolados da brusone do trigo. Entre as diversas gramíneas estudadas, os isolados de brusone provenientes de Brachiaria plantaginea e T. aestivum podem influenciar na alta variabilidade de M. grisea do trigo. Verificou-se variabilidade genética dentro de isolados de Setaria geniculata, sugerindo que a brusone desse hospedeiro pode influir na variabilidade da brusone do trigo. Isolados de brusone do arroz (Oryza sativa) apresentaram baixa fertilidade sexual.

scholarly journals A Gene-for-Gene Relationship Underlying the Species-Specific Parasitism of Avena/Triticum Isolates of Magnaporthe grisea on Wheat Cultivars

2002 ◽  
Vol 92 (11) ◽  
pp. 1182-1188 ◽  
Author(s):  
N. Takabayashi ◽  
Y. Tosa ◽  
H. S. Oh ◽  
S. Mayama
Keyword(s):  
Resistance Gene ◽  
Temperature Sensitivity ◽  
Chinese Spring ◽  
Magnaporthe Grisea ◽  
Gene Interactions ◽  
Wheat Cultivars ◽  
Genetic Mechanisms ◽  
Species Specific ◽  
Cultivar Specificity ◽  
Gene For Gene

To elucidate genetic mechanisms of the species-specific parasitism of Magnaporthe grisea, a Triticum isolate (pathogenic on wheat) was crossed with an Avena isolate (pathogenic on oat), and resulting F1 progeny were subjected to segregation analyses on wheat cvs. Norin 4 and Chinese Spring. We found two fungal loci, Pwt3 and Pwt4, which are involved in the specific parasitism on wheat. Pwt3 operated on both cultivars while Pwt4 operated only on ‘Norin 4’. Using the cultivar specificity of Pwt4, its corresponding resistance gene was successfully identified in ‘Norin 4’ and designated as Rmg1 (Rwt4). The presence of the corresponding resistance gene indicated that Pwt4 is an avirulence locus. Pwt3 was assumed to be an avirulence locus because of its temperature sensitivity. We suggest that gene-for-gene interactions underlie the species-specific parasitism of M. grisea.

scholarly journals Efecto de factores físicos y bioquímicos sobre la formación de estructuras infectivas de Magnaporthe grisea agente causal de pyriculiarosis en arroz (Oryza sativa)

2008 ◽  
Vol 2 (1) ◽  
pp. 31-36
Author(s):  
Wilber Salazar Antón
Keyword(s):  
Oryza Sativa ◽  
Magnaporthe Grisea

Este estudio se realizó en el laboratorio de Fitopatología de la Universidad de Kobe, Japón, evaluándose el efecto de dos factores físicos (superficies hidrofóbicas e hidrofílicas) y dos factores químicos (Adenosin Monofosfato cíclico (cAMP) y colagenasa) sobre la capacidad de adhesión y producción de apresorios de Magnaporthe grisea sobre superficies hidrofóbicas e hidrofílicas. En este estudio la concentración de conidias fue ajustada a 1x104 conidias/mililitro y se utilizó plástico Gelbond™ Film. Los resultados indican que sobre superficies hidrofóbicas el 78% de conidias produjeron apresorios, reduciéndose este porcentaje a cero cuando fueron colocadas sobre una superficie hidrofílica. Sin embargo, la adición de cAMP a las conidias creciendo sobre superficies hidrofílicas les restituyó la capacidad de producir apresorios hasta 69%. Cuando se aplicó colagenasa a la solución de conidias después de 6 horas de incubación su capacidad de adhesión se redujo hasta 61% en comparación con el tratamiento testigo sin colagenasa. Esta reducción fue más evidente cuando la solución de conidias se incubó por 6 horas junto con la colagenasa, reduciéndose el número de conidias adheridas a 97% en comparación con el testigo. La colagenasa también redujo la severidad de los síntomas de M. grisea en plántulas de arroz. La formación de estructuras infectivas de M. grisea se ve favorecida por superficies hidrofóbicas y la presencia de cAMP, en cambio, las superficies hidrofílicas y la colagenasa las inhiben.DOI: http://dx.doi.org/10.5377/universitas.v2i1.1641

scholarly journals A Set of Standard Differential Blast Isolates (Magnaporthe grisea (Hebert) Barr.) from the Philippines for Rice (Oryza sativa L.) Resistance

2008 ◽  
Vol 42 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Mary Jeanie TELEBANCO-YANORIA ◽  
Tokio IMBE ◽  
Hiroshi KATO ◽  
Hiroshi TSUNEMATSU ◽  
Leodegario A. EBRON ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Magnaporthe Grisea ◽  
Oryza Sativa L ◽  
The Philippines

Prediction of protein–protein interactions between fungus (Magnaporthe grisea) and rice (Oryza sativa L.)

2017 ◽  
Vol 20 (2) ◽  
pp. 448-456 ◽  
Author(s):  
Shiwei Ma ◽  
Qi Song ◽  
Huan Tao ◽  
Andrew Harrison ◽  
Shaobo Wang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Protein Interactions ◽  
Magnaporthe Grisea ◽  
Oryza Sativa L ◽  
Protein Protein Interactions

scholarly journals MGOS: A Resource for Studying Magnaporthe grisea and Oryza sativa Interactions

2006 ◽  
Vol 19 (10) ◽  
pp. 1055-1061 ◽  
Author(s):  
Carol Soderlund ◽  
Karl Haller ◽  
Vishal Pampanwar ◽  
Daniel Ebbole ◽  
Mark Farman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oryza Sativa ◽  
Fungal Pathogen ◽  
Magnaporthe Grisea ◽  
Genomic Sequence ◽  
Blast Disease ◽  
Infection Cycle ◽  
Community Based ◽  
Web Based ◽  
Expression Libraries

The MGOS (Magnaporthe grisea Oryza sativa) web-based database contains data from Oryza sativa and Magnaporthe grisea interaction experiments in which M. grisea is the fungal pathogen that causes the rice blast disease. In order to study the interactions, a consortium of fungal and rice geneticists was formed to construct a comprehensive set of experiments that would elucidate information about the gene expression of both rice and M. grisea during the infection cycle. These experiments included constructing and sequencing cDNA and robust long-serial analysis gene expression libraries from both host and pathogen during different stages of infection in both resistant and susceptible interactions, generating >50,000 M. grisea mutants and applying them to susceptible rice strains to test for pathogenicity, and constructing a dual O. sativa-M. grisea microarray. MGOS was developed as a central web-based repository for all the experimental data along with the rice and M. grisea genomic sequence. Community-based annotation is available for the M. grisea genes to aid in the study of the interactions.

scholarly journals Analysis of Host Species Specificity of Magnaporthe grisea Toward Foxtail Millet Using a Genetic Cross Between Isolates from Wheat and Foxtail Millet

2003 ◽  
Vol 93 (1) ◽  
pp. 42-45 ◽  
Author(s):  
J. Murakami ◽  
R. Tomita ◽  
T. Kataoka ◽  
H. Nakayashiki ◽  
Y. Tosa ◽  
...  
Keyword(s):  
Host Species ◽  
Magnaporthe Grisea ◽  
Foxtail Millet ◽  
Species Specificity ◽  
The Other ◽  
Single Locus ◽  
Gene Interactions ◽  
Genetic Cross ◽  
Genetic Mechanisms ◽  
Cultivar Specificity

Host species specificity of Magnaporthe grisea toward foxtail millet was analyzed using F1 cultures derived from a cross between a Triticum isolate (pathogenic on wheat) and a Setaria isolate (pathogenic on foxtail millet). On foxtail millet cvs. Beni-awa and Oke-awa, avirulent and virulent cultures segregated in a 1:1 ratio, suggesting that a single locus is involved in the specificity. This locus was designated as Pfm1. On cv. Ki-awa, two loci were involved and one of them was Pfm1. The other locus was designated as Pfm2. Interestingly, Pfm1 was not involved in the pathogenic specificity on cv. Kariwano-zairai. These results suggest that there is no “master gene” that determines the pathogenic specificity on all foxtail millet cultivars and that the species specificity of M. grisea toward foxtail millet is governed by cultivar-dependent genetic mechanisms that are similar to gene-for-gene interactions controlling race-cultivar specificity.

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