Nature of 2n gamete formation and mode of inheritance in interspecific hybrids of diploid Vaccinium darrowi and tetraploid V. corymbosum

1995 ◽  
Vol 91 (8) ◽  
pp. 1309-1315 ◽  
Author(s):  
L. Qu ◽  
J. F. Hancock
Keyword(s):  
Interspecific Hybrids ◽  
Gamete Formation ◽  
2N Gamete ◽  
Vaccinium Darrowi ◽  
Mode Of Inheritance

scholarly journals Nature of 2n Gamete Formation and Mode of Inheritance in Interspecific Hybrids of Diploid Vaccinium darrowii and Tetraploid V. corymbosum

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 808E-808
Author(s):  
Luping Qu ◽  
J.F. Hancock
Keyword(s):  
Interspecific Hybrids ◽  
Rapd Markers ◽  
2N Gametes ◽  
Diploid Population ◽  
A Value ◽  
Tetraploid Hybrid ◽  
Gamete Formation ◽  
2N Gamete ◽  
Segregating Population ◽  
Mode Of Inheritance

RAPD markers were used to determine the level of heterozygosity transmitted via 2n gametes from V. darrowi cv. Florida 4b (Fla 4B) to interspecific hybrids with tetraploid V. corymbosum cv. Bluecrop. The tetraploid hybrid US 75 was found to contain 70.6% of Fla 4B's heterozygosity, a value consistent with a first division restitution (FDR) mode of 2n gamete production. Crossovers during 2n gamete formation were evidenced by the absence of 16 dominant alleles of Fla 4B in US 75, and direct tests of segregation in a diploid population involving Fla 4B. RAPD markers that were present in both Fla 4B and US 75 were used to determine the mode of inheritance in a segregating population of US 75 × V. corymbosum cv. Bluetta. More than 30 homozygous pairs of alleles were located that segregated in a 5:1 ratio, indicating US 75 undergoes tetrasomic inheritance.

Determination of n+1 Gamete Transmission Rate of Trisomics and Location of Gene Controlling 2n Gamete Formation in Chinese Cabbage (Brassica rapa)

2009 ◽  
Vol 51 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Cheng-He Zhang ◽  
Xiao-Feng Li ◽  
Shu-Xing Shen ◽  
He Yuan ◽  
Shu-Xin Xuan
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Transmission Rate ◽  
Gamete Formation ◽  
2N Gamete

Genetic significance of mode of polyploidization: somatic doubling or 2n gametes?

Genome ◽  
1991 ◽  
Vol 34 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Kazuo Watanabe ◽  
Stanley J. Peloquin ◽  
Masatoshi Endo
Keyword(s):  
Combining Ability ◽  
Allelic Diversity ◽  
2N Gametes ◽  
Genotypic Diversity ◽  
P Value ◽  
Sexual Polyploidization ◽  
Gamete Formation ◽  
Single Exchange ◽  
Genetic Significance ◽  
2N Gamete

Computer simulation was undertaken to compare the genetic consequences of asexual (somatic doubling) and sexual (2n gametes) polyploidization. The coefficient of inbreeding at a locus, the number and frequency of genotypes at a locus, and the proportion of tri- and tetra-allelic genotypes were considered. The factors considered to estimate the genetic consequences were (i) mechanisms of sexual polyploidization, by first division restitution (FDR) × second division restitution (SDR), FDR × FDR, or SDR × SDR; (ii) position of the locus in relation to the centromere, which affects the gametic output in 2n gamete formation and thus the probability of single-exchange tetrads in meiosis during 2n gamete formation (p value); and (iii) allelic diversity at a locus. In comparing asexual and sexual polyploidization, regardless of the position of a locus in relation to the centromere, sexual polyploidization generally indicated less inbreeding, more genotypic diversity, and a higher proportion of tri- and tetra-allelic genotypes. When allelic diversity at a locus was increased, these characteristics were even more prominent. When only two alleles are possible at a locus, somatic doubling would not be inferior to sexual polyploidization. Overall results favored SDR × FDR and FDR × FDR as a mode and mechanisms of polyploidization. The genetic variations produced by 2n gametes could be attributed to "combining ability of 2n gametes."Key words: asexual polyploidization, sexual polyploidization, inbreeding, heterozygosity, combining ability of 2n gametes.

Maternal inheritance of the chloroplast genome in Eucalyptus globulus and interspecific hybrids

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 831-835 ◽  
Author(s):  
Gay E McKinnon ◽  
René E Vaillancourt ◽  
Paul A Tilyard ◽  
Brad M Potts
Keyword(s):  
Molecular Marker ◽  
Interspecific Hybrid ◽  
Eucalyptus Globulus ◽  
Interspecific Hybrids ◽  
Maternal Inheritance ◽  
Genetic Manipulation ◽  
Specific Marker ◽  
Genetic Studies ◽  
Rapid Pcr ◽  
Mode Of Inheritance

The utility of chloroplast DNA (cpDNA) in Eucalyptus, either as a molecular marker for genetic studies or as a potential vehicle for genetic manipulation, is based on knowledge of its mode of inheritance. Chloroplast inheritance in angiosperms can vary among and within species, and anomalous inheritance has been reported in some interspecific-hybrid combinations. In Eucalyptus, abnormalities of pollen-tube growth occur in a number of interspecific-hybrid combinations, and this might increase the likelihood of anomalous chloroplast transmission. We used a rapid PCR technique to determine chloroplast heritability in 425 progeny of Eucalyptus, comprising 194 progeny of the premier pulpwood species E. globulus and 231 interspecific hybrids between E. globulus and E. nitens (F1, F2, and backcrosses). At this sampling intensity, no pollen-mediated transmission of cpDNA was found in any of the 40 families tested. The results are discussed with reference to chloroplast engineering and the use of cpDNA as a seed-specific marker in phylogeographic studies of Eucalyptus.Key words: organelle, plastids, uniparental, maternal inheritance, interspecific hybrids.

scholarly journals 2n Gamete formation in the genus Brachiaria (Poaceae: Paniceae)

Euphytica ◽  
2006 ◽  
Vol 154 (1-2) ◽  
pp. 255-260 ◽  
Author(s):  
Patrícia Helena Gallo ◽  
Pamela Lonardoni Micheletti ◽  
Kellen Regina Boldrini ◽  
Claudicéia Risso-Pascotto ◽  
Maria Suely Pagliarini ◽  
...  
Keyword(s):  
Gamete Formation ◽  
2N Gamete

scholarly journals Genomics-based discrimination of 2n gamete formation mechanisms in polyploids: a case study in nonaploid Diospyros kaki ‘Akiou’

2021 ◽  
Author(s):  
Peng Sun ◽  
Soichiro Nishiyama ◽  
Hideaki Asakuma ◽  
Roeland E Voorrips ◽  
Jianmin Fu ◽  
...  
Keyword(s):  
2N Gametes ◽  
Unreduced Gametes ◽  
Formation Mechanisms ◽  
Common Source ◽  
Diospyros Kaki ◽  
Normal Female ◽  
Gamete Formation ◽  
Allele Dosage ◽  
2N Gamete ◽  
Gamete Production

Abstract Unreduced gametes (2n gametes), possessing double the haploid genome, whatever ploidy that happens to be, are a common source of ploidy variation in plant populations. First and second division restitution (FDR and SDR) are the dominant mechanisms of 2n gamete production; all else being equal, FDR gametes have a higher degree of heterozygosity, thus they are advantageous in breeding. The discrimination of these mechanisms from the consequence of hybridization is challenging, especially in higher polyploids, and usually requires information on centromere location. In this study, we propose a genotyping-based strategy to uncover the mechanisms of 2n gamete formation in progeny that has a higher ploidy than its parents. Simulation of 2n gamete production revealed that FDR and SDR pathways can be discriminated based on allele transmission patterns alone without information on centromere location. We applied this strategy to study the formation mechanism of a nonaploid Diospyros kaki ‘Akiou’, which was bred via hybridization between D. kaki hexaploid cultivars. The result demonstrated that ‘Akiou’ was derived from the fertilization of a normal female gamete by a 2n male gamete, and that this 2n gamete was produced through FDR. Consequently, the distinct duplex transmission pattern in the FDR gamete enabled us to infer the genomic characteristics of polyploid persimmon. The method could be tested only for the plant being polypoid, which allows for the ability to discriminate causes of 2n gamete formation using allele dosage in progeny, and will be useful in future studies of polyploid genomics.

Pollen vigor as a function of mode of 2n gamete formation in potatoes

1976 ◽  
Vol 67 (4) ◽  
pp. 204-208 ◽  
Author(s):  
P. W. SIMON ◽  
S. J. PELOQUIN
Keyword(s):  
Gamete Formation ◽  
2N Gamete ◽  
Pollen Vigor

A re-examination of the mechanisms of 2n gamete formation in potato and its implications for breeding

Euphytica ◽  
1979 ◽  
Vol 28 (3) ◽  
pp. 537-561 ◽  
Author(s):  
M. S. Ramanna
Keyword(s):  
Gamete Formation ◽  
2N Gamete

scholarly journals Randomly Amplified Polymorphic DNA- (RAPD-) based Genetic Linkage Map of Blueberry Derived from an Interspecific Cross between Diploid Vaccinium darrowi and Tetraploid V. corymbosum

1997 ◽  
Vol 122 (1) ◽  
pp. 69-73 ◽  
Author(s):  
Luping Qu ◽  
James F. Hancock
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Interspecific Cross ◽  
Randomly Amplified Polymorphic Dna ◽  
Tetraploid Hybrid ◽  
2N Gamete ◽  
Vaccinium Darrowi ◽  
Unique Markers ◽  
Selection Of

A tetraploid blueberry population resulting from a cross of US 75 {a tetraploid hybrid of Fla 4B [a selection of Vaccinium darrowi Camp (2n = 2x = 24) × `Bluecrop' [(V. corymbosum L. (2n = 4x = 48)]} × `Bluetta' (4x) was used to generate a genetic linkage map of US 75 by randomly amplified polymorphic DNA (RAPD) analysis. One hundred and forty markers unique for Fla 4B that segregated 1:1 in the population were mapped into 29 linkage groups that cover a total genetic distance of 1288.2 cM, with a range of 1.6 to 33.9 cM between adjacent markers. The map is essentially of V. darrowi because US 75 was produced via a 2n gamete from Fla 4B and only unique markers for Fla 4B were used. Therefore, all the chromosomes of V. darrowi could be represented in the map.

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