Chromosome localization and characterization of a family of long interspersed repetitive DNA elements from the genus Zea

1995 ◽  
Vol 90 (7-8) ◽  
pp. 1094-1100 ◽  
Author(s):  
R. Aledo ◽  
R. Raz ◽  
A. Monfort ◽  
C. M. Vicient ◽  
P. Puigdomènech ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Chromosome Localization ◽  
Dna Elements ◽  
Interspersed Repetitive Dna ◽  
Repetitive Dna Elements

Characterization of Repetitive DNA Elements in Arabidopsis

1999 ◽  
Vol 48 (6) ◽  
pp. 684-691 ◽  
Author(s):  
Stefan A. Surzycki ◽  
William R. Belknap
Keyword(s):  
Repetitive Dna ◽  
Dna Elements ◽  
Repetitive Dna Elements

Sequence Organization of Simple, Highly Repetitive DNA Elements inBrassicaspecies

1991 ◽  
Vol 42 (2) ◽  
pp. 243-249 ◽  
Author(s):  
D. R SIBSON ◽  
S. G. HUGHES ◽  
J. A. BRYANT ◽  
P. N. FITCHETT
Keyword(s):  
Repetitive Dna ◽  
Sequence Organization ◽  
Dna Elements ◽  
Highly Repetitive Dna ◽  
Repetitive Dna Elements

A rapid procedure for the isolation of C0t-1 DNA from plants

Genome ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 138-142 ◽  
Author(s):  
Michael S. Zwick ◽  
Robert E. Hanson ◽  
M. Nurul Islam-Faridi ◽  
David M. Stelly ◽  
Rod A. Wing ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Repetitive Dna ◽  
Copy Number ◽  
Genomic Dna ◽  
Mammalian Species ◽  
Repetitive Dnas ◽  
Rapid Procedure ◽  
Dna Elements ◽  
Repetitive Dna Elements

In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or "blocking" of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.Key words: C0t-1 DNA, in situ hybridization, BACs, plants.

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