The relationship between in vitro performance of haploid embryos and the agronomic performance of the derived doubled haploid lines in barley

1992 ◽  
Vol 84 (1-2) ◽  
pp. 118-122 ◽  
Author(s):  
R. Bozorgipour ◽  
J. W. Snape
Keyword(s):  
Doubled Haploid ◽  
Agronomic Performance ◽  
Doubled Haploid Lines ◽  
Haploid Embryos ◽  
The Relationship ◽  
In Vitro Performance

scholarly journals Induction of Parthenogenetic Haploid Embryos after Pollination by Irradiated Pollen in Watermelon

HortScience ◽  
1994 ◽  
Vol 29 (10) ◽  
pp. 1189-1190 ◽  
Author(s):  
N. Sari ◽  
K. Abak ◽  
M. Pitrat ◽  
J.C. Rode ◽  
R. Dumas de Vaulx
Keyword(s):  
In Vitro Culture ◽  
Doubled Haploid ◽  
Chromosome Doubling ◽  
Citrullus Lanatus ◽  
Doubled Haploid Lines ◽  
Irradiated Pollen ◽  
Haploid Embryos ◽  
Haploid Plants

Parthenogenetic haploid embryos of `Crimson Sweet', `Halep Karasi', `Sugar Baby' and `Panonia F1' watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] were obtained after pollination with γ-irradiated (200 or 300 Gy) pollen. Some globular and heart-shaped embryos were observed in fruit harvested 2 to 5 weeks after pollination. The number of embryos per 100 seeds was highest for `Halep Karasi'. After in vitro culture, 17 haploid plants were obtained and doubled haploid lines were generated after chromosome doubling using colchicine.

Agronomic Performance of Wheat Doubled-Haploid Lines Derived from Cultivars by Anther Culture

1989 ◽  
Vol 103 (2) ◽  
pp. 101-109 ◽  
Author(s):  
P. S. Baenziger ◽  
D. M. Wesenberg ◽  
V. M. Smail ◽  
W. L. Alexander ◽  
G. W. Schaeffer
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Agronomic Performance ◽  
Doubled Haploid Lines

scholarly journals In vitro androgenesis in triticale

2017 ◽  
Vol 1 (1) ◽  
pp. 99-100
Author(s):  
Oksana Ivanovna Zaitseva
Keyword(s):  
Genetic Variation ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Doubled Haploids ◽  
External Factors ◽  
Hexaploid Triticale ◽  
Doubled Haploid Lines ◽  
In Vitro Androgenesis

Abstract The technology for in vitro anther culturing was optimized in hexaploid triticale using combination of external factors that allowed to obtain more than 100 doubled haploid lines. Investigation of genetic variation among anther culture derived doubled haploids of triticale showed the occurrence of heterozygous plants.

scholarly journals Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture

2020 ◽  
Vol 56 (No. 4) ◽  
pp. 150-158
Author(s):  
Osama Zuhair Kanbar ◽  
Csaba Lantos ◽  
Paul Karumba Chege ◽  
Erzsébet Kiss ◽  
János Pauk
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Genetic Material ◽  
Triticum Aestivum L ◽  
Average Value ◽  
Doubled Haploid Lines ◽  
Dh Lines

We investigated the anther culture (AC) efficiency of thirteen F<sub>4</sub> combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25–87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.

scholarly journals Analysis of the intralinear polymorphism and the homozygosity rate of triticale doubled haploid lines by microsatellite markers

2020 ◽  
Vol 64 (2) ◽  
pp. 199-208
Author(s):  
A. V. Lagunovskaya ◽  
A. A. Buracova ◽  
V. N. Bushtevich ◽  
V. I. Sakovich ◽  
V. A. Lemesh ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Microsatellite Markers ◽  
Somaclonal Variation ◽  
Ssr Markers ◽  
Doubled Haploid ◽  
Microsatellite Loci ◽  
Doubled Haploids ◽  
Hexaploid Triticale ◽  
Doubled Haploid Lines

We evaluated the rate of polymorphism of doubled haploid lines of hexaploid triticale obtained by the method of anther culture based on hybrids of spring and winter types. Using 7 SSR markers for the loci on the chromosomes A- (Xgwm186, Xgwm291, Xgwm595) and B- (Xgwm371, Xgwm540, Xgwm554, Xgwm234), polymorphism of 38 doubled haploid lines of hexaploid triticale was studied. Interlinear polymorphism along six microsatellite loci except the Xgwm554 locus, which is not polymorphic in the studied doubled haploid lines, was revealed. The highest polymorphism was observed for the Xgwm186, Xgwm291 and Xgwm595 loci. The cluster analysis showed that all studied lines were divided into three main groups. The origin of the lines did not affect the distribution in groups. This confirms the influence of in vitro culture somaclonal variation. Eight lines of doubled haploids, which are heterozygous for one of the studied microsatellite loci, were identified. We showed the possibility of using SSR markers to assess interlinear polymorphism and the homozygosity rate in the triticale doubled haploid lines obtained by the method of induced androgenesis in vitro.

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