Glycosphingolipids: The putative receptor for staphylococcus aureus enterotoxin-B in human kidney proximal tubular cells

1992 ◽  
Vol 113 (1) ◽  
Author(s):  
Subroto Chatterjee ◽  
Marti Jett
Keyword(s):  
Staphylococcus Aureus ◽  
Human Kidney ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Putative Receptor ◽  
Proximal Tubular ◽  
Enterotoxin B ◽  
Kidney Proximal Tubular Cells ◽  
Staphylococcus Aureus Enterotoxin B

scholarly journals Identification of Staphylococcal Enterotoxin B Domains Involved in Binding to Cultured Human Kidney Proximal Tubular Cells: Imparting Proliferation and Death

2007 ◽  
Vol 232 (9) ◽  
pp. 1142-1151 ◽  
Author(s):  
Subroto Chatterjee ◽  
Roger Neill ◽  
Jeffrey W. Shupp ◽  
Rasha Hammamieh ◽  
Boris Ionin ◽  
...  
Keyword(s):  
Human Kidney ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Functional Studies ◽  
Putative Receptor ◽  
Proximal Tubular ◽  
Enterotoxin B ◽  
Kidney Proximal Tubular Cells

Studies suggest that staphylococcal enterotoxin B (SEB) is initially harbored in the kidney by binding to digalactosylceramide molecules in the proximal tubular cells. However, little is known in regard to the peptide motif within SEB that binds to these cells and imparts toxic effects. Herein, using human kidney proximal tubular cells (PTs) we have performed a systematic study on the binding of various peptides and peptide analogs of SEB and demonstrate a structure-functional relationship. Using [125I]labeled SEB peptides, we show a high affinity and displaceable binding of SEB 191–220 to human PT cells. Binding was mitigated by the use of antibody against SEB, by digalactosylceramide (the putative receptor), and by the use of endoglycoceramidase, which selectively removes the oligosaccharide backbones from glycosphingolipids. Our structure/ functional studies revealed that peptide 130–160 induces a concentration-dependent increase in programmed cell death/ apoptosis in human proximal tubular cells. Mechanistic studies further suggest that SEB/SEB peptide (130–160) impart apoptosis via the activation of neutral sphingomyelinase, which hydrolizes sphingomyelin to ceramide and phosphocholine. SEB 130–160 mediated apoptosis was mitigated by preincubation of cells with antibody against SEB and an SEB 130–160 antibody.

scholarly journals Structure–Interaction Relationship of Polymyxins with the Membrane of Human Kidney Proximal Tubular Cells

2020 ◽  
Vol 6 (8) ◽  
pp. 2110-2119 ◽  
Author(s):  
Xukai Jiang ◽  
Shuo Zhang ◽  
Mohammad A. K. Azad ◽  
Kade D. Roberts ◽  
Lin Wan ◽  
...  
Keyword(s):  
Human Kidney ◽  
Proximal Tubular Cells ◽  
Structure Interaction ◽  
Tubular Cells ◽  
Proximal Tubular ◽  
Kidney Proximal Tubular Cells ◽  
Relationship Of ◽  
Interaction Relationship

scholarly journals Effect of TIMP1 transfection on PTEN expression in human kidney proximal tubular cells

2015 ◽  
Vol 14 (4) ◽  
pp. 17373-17383 ◽  
Author(s):  
J.X. Chen ◽  
G.Y. Cai ◽  
X.M. Chen ◽  
H. Liu ◽  
X. Chen ◽  
...  
Keyword(s):  
Human Kidney ◽  
Pten Expression ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Proximal Tubular ◽  
Kidney Proximal Tubular Cells

scholarly journals Effects of SGLT2 Inhibition in Human Kidney Proximal Tubular Cells—Renoprotection in Diabetic Nephropathy?

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e54442 ◽  
Author(s):  
Usha Panchapakesan ◽  
Kate Pegg ◽  
Simon Gross ◽  
Muralikrishna Gangadharan Komala ◽  
Harshini Mudaliar ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Human Kidney ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Sglt2 Inhibition ◽  
Proximal Tubular ◽  
Kidney Proximal Tubular Cells
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