Transfer of dye among salivary gland cells is not affected by genetic variations of the period clock gene in Drosophila melanogaster

1993 ◽  
Vol 136 (3) ◽  
Author(s):  
KimberlyK. Flint ◽  
Michael Rosbash ◽  
JeffreyC. Hall
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
Clock Gene ◽  
Genetic Variations ◽  
Gland Cells ◽  
Salivary Gland Cells

WHOLE MOUNT ELECTRON MICROSCOPY OF THE NUCLEOLUS IN SALIVARY GLAND CELLS OF DROSOPHILA MELANOGASTER

1977 ◽  
Vol 19 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Gary D. Burkholder
Keyword(s):  
Electron Microscopy ◽  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
Peripheral Region ◽  
Core Region ◽  
28S Rrna ◽  
The Core ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Whole Mount

The nucleolus of Drosophila melanogaster salivary gland cells, examined by whole mount electron microscopy, consists of a fibrillar core region and a peripheral region containing both fibres and granules. These regions appear to correspond to the fibrillar and granular components, respectively, seen in thin sections. Most of the nucleoli were attached to the chromocenter region of the polytene chromosomes, containing the nucleolar organizer. Bundles of relatively straight chromatin fibres, 13 nm in diameter, extended from the chromocenter into the core region of the nucleolus, however it was not possible to trace the path of these chromatin fibres through the nucleolus since they were obscured within the mass of nucleolar fibres. The nucleolar fibres in both the core and peripheral regions were irregular and knobby, with a diameter of about 15 nm. In the core region, the fibres appeared to be of considerable length and were characteristically clustered together to form small interconnected masses. The fibres in the peripheral region were relatively short and some appeared to blend with amorphous, poorly-defined pools of material. Electron dense granules 15-20 nm in diameter were also associated with this amorphous substance. It is hypothesized that the formation and subsequent packaging of the 28s rRNA may be represented by a morphological transition of the peripheral fibres, via an amorphous pool-like intermediate stage, into the nucleolar granules. The results of this study indicate that whole mount electron microscopy may be a useful alternative to thin sectioning in high resolution studies of the nucleolus.

scholarly journals CHROMOSOME-NUCLEAR MEMBRANE-CYTOPLASMIC INTERRELATIONS IN DROSOPHILA

1956 ◽  
Vol 2 (4) ◽  
pp. 407-414 ◽  
Author(s):  
Helen Gay
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
Larval Development ◽  
Electron Micrographs ◽  
Nuclear Membrane ◽  
Membrane Structure ◽  
Deoxyribonucleic Acid ◽  
Cellular Function ◽  
Gland Cells ◽  
Salivary Gland Cells

The structural evidence for nucleocytoplasmic interrelationships observed in electron micrographs of salivary-gland cells of third instar larvae of Drosophila melanogaster has been reviewed. It has been found that the characteristic nuclear membrane outpocketings with their adjacent highly differentiated chromosomal materials occur at one stage of larval development at a time when a new cellular function is being initiated. Preliminary cytochemical studies to characterize the materials transferred from nucleus to cytoplasm indicate that deoxyribonucleic acid occurs within the blebs. Observations on chromosome and nuclear membrane structure are also presented.

Rapid activation of calcium-sensitive Na+H+ exchange induced by 20-hydroxyecdysone in salivary gland cells of Drosophila melanogaster

1996 ◽  
Vol 116 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Stefan Schneider ◽  
Stefan Wünsch ◽  
Albrecht Schwab ◽  
Hans Oberleithner
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Rapid Activation

Glycogen distribution in the larval salivary gland cells during the development of Drosophila melanogaster and Drosophila auraria: an ultrastructural cytochemical study

2000 ◽  
Vol 251 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Artemis Kosta ◽  
Katerina Dimopoulou ◽  
Victoria Drosou ◽  
George N. Thomopoulos
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
Cytochemical Study ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Larval Salivary Gland

An ultrastructural and histochemical study of the secretory granules in the salivary gland cells of the Drosophila melanogaster species subgroup: phylogenetic relationships and evolutionary aspects

1987 ◽  
Vol 65 (4) ◽  
pp. 950-957 ◽  
Author(s):  
G. N. Thomopoulos
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
Granular Material ◽  
Secretory Granules ◽  
Ultrastructural Level ◽  
Structural Level ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Complex Carbohydrates ◽  
Filamentous Material

The morphology of the secretory granules and the distribution of "neutral" complex carbohydrates in these structures of the larval salivary gland cells of six species belonging to the melanogaster subgroup of Drosophila were examined at the ultra-structural level. The secretory granules of each species show a unique morphology. The species of the melanogaster subgroup can be subdivided into two complexes (the melanogaster and the yakuba complexes) on the basis of the existence of filamentous or granular material in their secretory granules. In the former complex belong the species Drosophila melanogaster, Drosophila simulans, and Drosophila mauritiana, whose secretory granules consist mainly of filamentous material, and in the latter complex belong the species Drosophila yakuba, Drosophila teissieri, and Drosophila erecta, whose secretory granules consist of granular material. A phylogeny of the six species, in which D. erecta is regarded as the most primitive, is presented. It is proposed that the morphology of the secretory granules at the ultrastructural level, combined with the distribution of complex carbohydrates in them, can be used as an additional criterion for the classification of the Drosophila species.

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