Efficient in vitro regeneration of leek (Allium ampeloprasum L.) via flower stalk segments

1995 ◽  
Vol 14 (7) ◽  
pp. 423-427 ◽  
Author(s):  
B. Silvertand ◽  
E. Jacobsen ◽  
J. Mazereeuw ◽  
P. Lavrijsen ◽  
A. van Harten
Keyword(s):  
In Vitro Regeneration ◽  
Flower Stalk ◽  
Allium Ampeloprasum

scholarly journals In vitro Propagation in Three Varieties of Gerbera (Gerbera jamesonii Bolus.) from Flower Bud and Flower Stalk Explants

2013 ◽  
Vol 22 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Nazma Akter ◽  
MI Hoque ◽  
RH Sarker
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Root Induction ◽  
Gerbera Jamesonii ◽  
Flower Bud ◽  
Flower Stalk ◽  
Best Response ◽  
In Vitro Shoots

Three types of tender leaves derived explants such as leaf tip, leaf with mid-rib and leaf blade segments as well as flower buds and flower stalks obtained from three selected varieties of gerbera (Gerbera jamesonii Bolus.) cultivated in Bangladesh were exploited for callus induction and in vitro regeneration of plantlets. Among the explants flower bud and flower stalk were suitable and superior for callus induction and subsequent regeneration of in vitro shoots when cultured on MS supplemented with 5.0 mg/l BAP and 1.0 mg/l NAA. However, highest number of multiple shoots were obtained when the flower bud derived callus was subcultured on MS supplemented with 2.0 mg/l BAP. Variety with red petal showed the best response in producing multiple shoots among the three varieties. Root induction at the base of the in vitro regenerated shoots was tried using full and half the strengths of MS containing different concentrations of IBA. Best root induction was observed on half the strength of MS supplemented with 0.2 mg/l IBA. Following adequate acclimatization the regenerated plantlets were successfully transferred to soil where they grew to maturity and produced flowers. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14203 Plant Tissue Cult. & Biotech. 22(2): 143-152, 2012 (December)

Direct in vitro Regeneration of Arachis Hypogaea L.(Jl-24) and Study of Salt Tolerance

2011 ◽  
Vol 3 (8) ◽  
pp. 52-54 ◽  
Author(s):  
Bhagya Lakshmi Jyothi Kusuma ◽  
◽  
Sharmila Begum S
Keyword(s):  
Salt Tolerance ◽  
Arachis Hypogaea ◽  
In Vitro Regeneration ◽  
Arachis Hypogaea L

In vitro regeneration of watermelon seed segments

2015 ◽  
Vol 4 (8) ◽  
Author(s):  
M. Ameri ◽  
M. Lahouti ◽  
A. Bagheri ◽  
A. Sharifi ◽  
F. Keykha Akhar
Keyword(s):  
In Vitro Regeneration ◽  
Watermelon Seed

In vitro regeneration of Phaseolus vulgaris L. via direct and indirect organogenesis

2021 ◽  
Author(s):  
Yan Yu ◽  
Dajun Liu ◽  
Chang Liu ◽  
Zhishan Yan ◽  
Xiaoxu Yang ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
In Vitro Regeneration ◽  
Phaseolus Vulgaris L ◽  
Indirect Organogenesis

scholarly journals Ovaries of Chrysanthemum Irradiated with High-Energy Photons and High-Energy Electrons Can Regenerate Plants with Novel Traits

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1111
Author(s):  
Natalia Miler ◽  
Iwona Jedrzejczyk ◽  
Seweryn Jakubowski ◽  
Janusz Winiecki
Keyword(s):  
Total Dose ◽  
In Vitro Regeneration ◽  
High Energy ◽  
Mutation Breeding ◽  
Chrysanthemum Morifolium ◽  
Physical Factors ◽  
Strong Impact ◽  
Breeding Method ◽  
High Energy Electrons

Classical mutation breeding using physical factors is a common breeding method for ornamental crops. The aim of our study was to examine the utility of ovaries excised from irradiated inflorescences of Chrysanthemum × morifolium (Ramat.) as explants for breeding purposes. We studied the in vitro regeneration capacity of the ovaries of two chrysanthemum cultivars: ‘Profesor Jerzy’ and ‘Karolina’ preceded by irradiation with high-energy photons (total dose 5, 10 and 15 Gy) and high-energy electrons (total dose 10 Gy). Growth and inflorescence parameters of greenhouse acclimatized regenerants were recorded, and ploidy level was estimated with flow cytometry. The strong impact of genotype on regeneration efficiency was recorded—cultivar ‘Karolina’ produced only 7 viable shoots, while ‘Profesor Jerzy’ produced totally 428 shoots. With an increase of irradiation dose, the regeneration decreased, the least responsive were explants irradiated with 15 Gy high-energy photons and 10 Gy high-energy electrons. Regenerants of ‘Profesor Jerzy’ obtained from these explants possessed shorter stem and flowered later. The highest number of stable, color and shape inflorescence variations were obtained from explants treated with 10 Gy high-energy photons. Variations of inflorescences were predominantly changes of shape—from full to semi-full. New color phenotypes were dark yellow, light yellow and pinkish, among them only the dark yellow phenotype remained stable during second year cultivation. None of the regenerants were haploid. The application of ovaries irradiated within the whole inflorescence of chrysanthemum can be successfully applied in the breeding programs, provided the mother cultivar regenerate in vitro efficiently.

In vitro regeneration of the colorful fern Pteris aspericaulis var. tricolor via green globular bodies system

2021 ◽  
Vol 57 (2) ◽  
pp. 225-234
Author(s):  
Rongpei Yu ◽  
Fan Li ◽  
Guoxian Wang ◽  
Jiwei Ruan ◽  
Lifang Wu ◽  
...  
Keyword(s):  
In Vitro Regeneration
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