Production of S-lactoylglutathione by glycerol-adapted Saccharomyces cerevisiae and genetically engineered Escherichia coli cells

1988 ◽  
Vol 28 (3) ◽  
Author(s):  
N. Kosugi ◽  
Y. Inoue ◽  
H.-I. Rhee ◽  
K. Murata ◽  
A. Kimura
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Genetically Engineered ◽  
Escherichia Coli Cells ◽  
Engineered Escherichia Coli

Studies of operational variables in batch mode for genetically engineered Escherichia coli cells containing penicillin acylase

1993 ◽  
Vol 15 (12) ◽  
pp. 1070-1073 ◽  
Author(s):  
S. Bhattacharya ◽  
V.S. Gupta ◽  
A.A. Prabhune ◽  
H. SivaRaman ◽  
M. Debnath ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Penicillin Acylase ◽  
Genetically Engineered ◽  
Batch Mode ◽  
Escherichia Coli Cells ◽  
Operational Variables ◽  
Engineered Escherichia Coli

Biodecomposition of Phenanthrene and Pyrene by a Genetically Engineered Escherichia coli

2020 ◽  
Vol 14 (2) ◽  
pp. 121-133 ◽  
Author(s):  
Maryam Ahankoub ◽  
Gashtasb Mardani ◽  
Payam Ghasemi-Dehkordi ◽  
Ameneh Mehri-Ghahfarrokhi ◽  
Abbas Doosti ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Performance ◽  
Human Cancer ◽  
Genetically Engineered ◽  
Hazardous Substances ◽  
E Coli ◽  
Spiked Soil ◽  
Engineered Escherichia Coli ◽  
Genetically Manipulated ◽  
Hplc Measurement

Background: Genetically engineered microorganisms (GEMs) can be used for bioremediation of the biological pollutants into nonhazardous or less-hazardous substances, at lower cost. Polycyclic aromatic hydrocarbons (PAHs) are one of these contaminants that associated with a risk of human cancer development. Genetically engineered E. coli that encoded catechol 2,3- dioxygenase (C230) was created and investigated its ability to biodecomposition of phenanthrene and pyrene in spiked soil using high-performance liquid chromatography (HPLC) measurement. We revised patents documents relating to the use of GEMs for bioremediation. This approach have already been done in others studies although using other genes codifying for same catechol degradation approach. Objective: In this study, we investigated biodecomposition of phenanthrene and pyrene by a genetically engineered Escherichia coli. Methods: Briefly, following the cloning of C230 gene (nahH) into pUC18 vector and transformation into E. coli Top10F, the complementary tests, including catalase, oxidase and PCR were used as on isolated bacteria from spiked soil. Results: The results of HPLC measurement showed that in spiked soil containing engineered E. coli, biodegradation of phenanthrene and pyrene comparing to autoclaved soil that inoculated by wild type of E. coli and normal soil group with natural microbial flora, were statistically significant (p<0.05). Moreover, catalase test was positive while the oxidase tests were negative. Conclusion: These findings indicated that genetically manipulated E. coli can provide an effective clean-up process on PAH compounds and it is useful for bioremediation of environmental pollution with petrochemical products.

Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli

2013 ◽  
Vol 35 (5) ◽  
pp. 689-693 ◽  
Author(s):  
Baosheng Ge ◽  
Yan Li ◽  
Haixiang Sun ◽  
Shuai Zhang ◽  
Peijie Hu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetically Engineered ◽  
Engineered Escherichia Coli

Optimizing a production strategy for a nonspecific nuclease from Yersinia enterocolitica subsp. palearctica in genetically engineered Escherichia coli

2019 ◽  
Vol 366 (24) ◽  
Author(s):  
Yan Ge ◽  
Senlin Guo ◽  
Tao Liu ◽  
Chen Zhao ◽  
Duanhua Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Yersinia Enterocolitica ◽  
Inclusion Bodies ◽  
Genetically Engineered ◽  
Biological Research ◽  
Dilution Method ◽  
E Coli ◽  
Protein Renaturation ◽  
Engineered Escherichia Coli ◽  
Pharmaceutical Production

ABSTRACT A nuclease from Yersinia enterocolitica subsp. palearctica (Nucyep) is a newly found thermostable nonspecific nuclease. The heat-resisting ability of this nuclease would be extremely useful in biological research or pharmaceutical production. However, the application of this nuclease is limited because of its poor yield. This research aimed to improve Nucyep productivity by producing a novel genetically engineered Escherichia coli and optimizing the production procedures. After 4 h of induction by lactose, the new genetically engineered E. coli can express a substantial amount of Nucyep in the form of inclusion bodies. The yield was approximately 0.3 g of inclusion bodies in 1 g of bacterial pellets. The inclusion bodies were extracted by sonication and solubilized in an 8 M urea buffer. Protein renaturation was successfully achieved by dilution method. Pure enzyme was obtained after subjecting the protein solution to anion exchange. The Nucyep showed its nonspecific and heat resistant properties as previously reported (Boissinot et  al. 2016). Through a quantification method, its activity was determined to be 1.3 × 10 6 Kunitz units (K.U.)/mg. These results can serve as a reference for increasing Nucyep production.

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