Basis and extent of genetic variability among doubled haploid plants obtained by pollen culture in Nicotiana sylvestris

1981 ◽  
Vol 59 (3) ◽  
pp. 177-184 ◽  
Author(s):  
R. De Paepe ◽  
D. Bleton ◽  
F. Gnangbe
Keyword(s):  
Genetic Variability ◽  
Doubled Haploid ◽  
Nicotiana Sylvestris ◽  
Pollen Culture ◽  
Doubled Haploid Plants ◽  
Haploid Plants

Effects of consecutive androgeneses on morphology and fertility in Nicotiana sylvestris

1983 ◽  
Vol 61 (7) ◽  
pp. 2038-2046 ◽  
Author(s):  
R. De Paepe ◽  
D. Prat ◽  
J. Knight
Keyword(s):  
Doubled Haploid ◽  
Pollen Fertility ◽  
Doubled Haploids ◽  
Pollen Grains ◽  
Nicotiana Sylvestris ◽  
Somatic Tissue ◽  
Source Line ◽  
Doubled Haploid Plants ◽  
Androgenetic Haploid ◽  
Haploid Plants

In Nicotiana sylvestris androgenetic haploid and doubled-haploid plants regenerated from isolated pollen grains show characteristic modifications in leaves and flowers as compared with the pollen source line. During successive cycles of androgenesis, their average dimensions decrease regularly up to the fifth cycle. Afterwards no significant decrease is observed, but other kinds of abnormalities appear, such as foliar outgrowths developing from leaf veins, in haploid and doubled-haploid plants, or from the somatic tissue of the ovules in haploids only. Doubled-haploid plants transmit their abnormalities through the first and second generations of selfing. Their fertility is greatly reduced because of physiological deficiencies when the plants are used as females, and low pollen fertility. Pollen from doubled haploids also has inferior androgenic capabilities.

scholarly journals Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

2016 ◽  
Vol 44 (2) ◽  
pp. 133
Author(s):  
Cucu Gunarsih ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Muhamad Syukur
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Callus Induction ◽  
Rainfed Rice ◽  
Randomized Design ◽  
Doubled Haploid Plants ◽  
Completely Randomized Design ◽  
N6 Medium ◽  
Haploid Plants

ABSTRACT<br /><br />The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing:  INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM  putresin, and media for rooting was based on  MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and  Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%,  respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants),  G5 (21 plants), and G6 (26 plants).<br /><br />Keywords: Callus induction, doubled haploid, rice<br /><br />

FIRST DOUBLED HAPLOID PLANTS OF PEAR (PYRUS COMMUNIS)

2002 ◽  
pp. 173-175 ◽  
Author(s):  
L. Bouvier ◽  
P. Guerif ◽  
M. Djulbic ◽  
Y. Lespinasse
Keyword(s):  
Doubled Haploid ◽  
Pyrus Communis ◽  
Doubled Haploid Plants ◽  
Haploid Plants

scholarly journals Doubled haploids of interspecific hybrids between Brassica napus and Brassica rapa for canola production with valuable breeding traits

OCL ◽  
2020 ◽  
Vol 27 ◽  
pp. 45
Author(s):  
Ainash Daurova ◽  
Dias Daurov ◽  
Dmitriy Volkov ◽  
Kuanysh Zhapar ◽  
Daniyar Raimbek ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Brassica Rapa ◽  
Doubled Haploid ◽  
Interspecific Hybrids ◽  
Nutritional Value ◽  
Doubled Haploids ◽  
Doubled Haploid Plants ◽  
Haploid Plants

Doubled haploids (DH) were obtained from two interspecific hybrids between Brassica napus and Brassica rapa. Seeds of doubled haploid plants differed in colour and size. The hybridity of the obtained doubled haploid is shown using genomic in situ hybridization (GISH) analysis. Evaluation of drought tolerance during seed germination on PEG-6000 showed the advantage of doubled haploid plants of interspecific hybrids over the parent cultivars. The oil from seeds of doubled haploid plants showed good nutritional value.

scholarly journals Factors Affecting Doubled Haploid Plant Production Via Maize Technique in Bread Wheat

2015 ◽  
Vol 56 (2) ◽  
pp. 67-73
Author(s):  
Ioannis Xynias ◽  
Antonios Koufalis ◽  
Evdokia Gouli-Vavdinoudi ◽  
Demetrios Roupakias
Keyword(s):  
Bread Wheat ◽  
Doubled Haploid ◽  
Embryo Rescue ◽  
Plant Production ◽  
Haploid Plant ◽  
In Planta ◽  
Maize Pollen ◽  
Doubled Haploid Plants ◽  
Haploid Embryos ◽  
Haploid Plants

Abstract The effect of two in planta factors (growth conditions, genotype) and two in vitro factors (time of embryo rescue, embryo rescue medium) on doubled haploid (DH) plant production in bread wheat via maize technique was investigated in nine F1 hybrids produced after crossing four bread wheat cultivars. During the first year one group of F1 plants was grown in a field and at the proper stage pollinated with maize pollen (sweet corn popu-lation). In parallel, a second group of F1 plants was grown in a growth chamber and pollinated as in the former group. In the second growing season the experiment was repeated but only field-grown plants were used. All the produced haploid embryos were cultured in three different media and the resulting 146 haploid plants were sub-sequently treated with aqueous solution of colchicine. Finally, 86 doubled haploid plants were obtained. We noted that the growing conditions of the parental plants and the intervening time between day of pollination and day of embryo rescue influenced the percentage of haploid embryo production. Culture medium also influenced haploid and doubled haploid plant production. The two media (MS/2, B5) were found equally effective. Most of the haploid embryos originated from the Penios × Acheloos cross, whereas most of the doubled haploid plants were produced from the KVZ × Penios cross. Doubled haploid plants were produced from all crosses.

Production of doubled haploid plants of carnation (Dianthus caryophyllus L.) by pseudofertilized ovule culture

2000 ◽  
Vol 83 (3-4) ◽  
pp. 301-310 ◽  
Author(s):  
S. Sato ◽  
N. Katoh ◽  
H. Yoshida ◽  
S. Iwai ◽  
M. Hagimori
Keyword(s):  
Doubled Haploid ◽  
Dianthus Caryophyllus ◽  
Ovule Culture ◽  
Doubled Haploid Plants ◽  
Haploid Plants
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