Application of trifluralin to embryogenic microspore cultures to generate doubled haploid plants in Brassica napus

1995 ◽  
Vol 95 (2) ◽  
pp. 304-309 ◽  
Author(s):  
Jiping Zhao ◽  
Daina H. Simmonds
Keyword(s):  
Brassica Napus ◽  
Doubled Haploid ◽  
Doubled Haploid Plants ◽  
Haploid Plants

scholarly journals Doubled haploids of interspecific hybrids between Brassica napus and Brassica rapa for canola production with valuable breeding traits

OCL ◽  
2020 ◽  
Vol 27 ◽  
pp. 45
Author(s):  
Ainash Daurova ◽  
Dias Daurov ◽  
Dmitriy Volkov ◽  
Kuanysh Zhapar ◽  
Daniyar Raimbek ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Brassica Rapa ◽  
Doubled Haploid ◽  
Interspecific Hybrids ◽  
Nutritional Value ◽  
Doubled Haploids ◽  
Doubled Haploid Plants ◽  
Haploid Plants

Doubled haploids (DH) were obtained from two interspecific hybrids between Brassica napus and Brassica rapa. Seeds of doubled haploid plants differed in colour and size. The hybridity of the obtained doubled haploid is shown using genomic in situ hybridization (GISH) analysis. Evaluation of drought tolerance during seed germination on PEG-6000 showed the advantage of doubled haploid plants of interspecific hybrids over the parent cultivars. The oil from seeds of doubled haploid plants showed good nutritional value.

scholarly journals Effects of Heat Shock and 2, 4-D Treatment on Morphological and Physiological Characteristics of Microspores and Microspore-Derived Doubled Haploid Plants in Brassica napus L.

2015 ◽  
Vol 13 (2) ◽  
pp. 31-38 ◽  
Author(s):  
Fatemeh Pourabdollah Najafabadi ◽  
Mehran Enayati Shariatpanahi ◽  
Behzad Ahmadi ◽  
NayerazamKhosh-Kholgh Sima ◽  
Bahram Alizadeh ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Heat Shock ◽  
Doubled Haploid ◽  
Physiological Characteristics ◽  
Brassica Napus L ◽  
Doubled Haploid Plants ◽  
Haploid Plants

scholarly journals Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

2016 ◽  
Vol 44 (2) ◽  
pp. 133
Author(s):  
Cucu Gunarsih ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Muhamad Syukur
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Callus Induction ◽  
Rainfed Rice ◽  
Randomized Design ◽  
Doubled Haploid Plants ◽  
Completely Randomized Design ◽  
N6 Medium ◽  
Haploid Plants

ABSTRACT<br /><br />The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing:  INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM  putresin, and media for rooting was based on  MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and  Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%,  respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants),  G5 (21 plants), and G6 (26 plants).<br /><br />Keywords: Callus induction, doubled haploid, rice<br /><br />

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