Replication of Fpoh + plasmid in mafA mutants of Escherichia coli defective in plasmid maintenance

1977 ◽  
Vol 152 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Chieko Wada ◽  
Takashi Yura ◽  
Sota Hiraga
Keyword(s):  
Escherichia Coli ◽  
Plasmid Maintenance

Global transcriptional analysis of metabolic burden due to plasmid maintenance in Escherichia coli DH5α during batch fermentation

2006 ◽  
Vol 39 (3) ◽  
pp. 391-398 ◽  
Author(s):  
Dave Siak-Wei Ow ◽  
Peter Morin Nissom ◽  
Robin Philp ◽  
Steve Kah-Weng Oh ◽  
Miranda Gek-Sim Yap
Keyword(s):  
Escherichia Coli ◽  
Batch Fermentation ◽  
Transcriptional Analysis ◽  
Plasmid Maintenance ◽  
Metabolic Burden

Plasmid maintenance and physiology of a genetically engineered Escherichia coli strain during continuous l-carnitine production

2007 ◽  
Vol 29 (10) ◽  
pp. 1549-1556 ◽  
Author(s):  
Vicente Bernal ◽  
María González-Veracruz ◽  
Manuel Cánovas ◽  
José L. Iborra
Keyword(s):  
Escherichia Coli ◽  
Coli Strain ◽  
Genetically Engineered ◽  
Plasmid Maintenance ◽  
Engineered Escherichia Coli

Stable maintenance in chemostat-grown Escherichia coli of pBR322 and pACYC184 by disruption of the tetracycline resistance gene*

1985 ◽  
Vol 5 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Thierry Vernet ◽  
Ian J. McDonald ◽  
Dave R. Cameron ◽  
Louis P. Visentin
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Plasmid Stability ◽  
Tetracycline Resistance ◽  
Cloning Vector ◽  
Tetracycline Resistance Gene ◽  
Plasmid Maintenance ◽  
Stable Maintenance ◽  
Vector Pbr322

Plasmid stability was studied in antibiotic-free chemo-stat cultures. Disruption, either by deletion or insertion, of the tetracycline resistance gene in the EcoRl/EcoRV region of the cloning vector pBR322 or in the HindIII]BamHl region of pACYCI84 yields plasmids markedly more stable than the parent plasmids. Thus, at least for these two instances, cloning of a partitioning (par) locus is not prerequisite for plasmid maintenance.

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