Stable maintenance in chemostat-grown Escherichia coli of pBR322 and pACYC184 by disruption of the tetracycline resistance gene*

1985 ◽  
Vol 5 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Thierry Vernet ◽  
Ian J. McDonald ◽  
Dave R. Cameron ◽  
Louis P. Visentin
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Plasmid Stability ◽  
Tetracycline Resistance ◽  
Cloning Vector ◽  
Tetracycline Resistance Gene ◽  
Plasmid Maintenance ◽  
Stable Maintenance ◽  
Vector Pbr322

Plasmid stability was studied in antibiotic-free chemo-stat cultures. Disruption, either by deletion or insertion, of the tetracycline resistance gene in the EcoRl/EcoRV region of the cloning vector pBR322 or in the HindIII]BamHl region of pACYCI84 yields plasmids markedly more stable than the parent plasmids. Thus, at least for these two instances, cloning of a partitioning (par) locus is not prerequisite for plasmid maintenance.

scholarly journals Prevalence of Antimicrobial Resistance and Transfer of Tetracycline Resistance Genes in Escherichia coli Isolates from Beef Cattle

2015 ◽  
Vol 81 (16) ◽  
pp. 5560-5566 ◽  
Author(s):  
Seung Won Shin ◽  
Min Kyoung Shin ◽  
Myunghwan Jung ◽  
Kuastros Mekonnen Belaynehe ◽  
Han Sang Yoo
Keyword(s):  
Escherichia Coli ◽  
South Korea ◽  
Beef Cattle ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Tetracycline Resistance ◽  
Tetracycline Resistance Gene ◽  
Content Type ◽  
E Coli ◽  
Tetracycline Resistance Genes

ABSTRACTThe aim of this study was to investigate the prevalence and transferability of resistance in tetracycline-resistantEscherichia coliisolates recovered from beef cattle in South Korea. A total of 155E. coliisolates were collected from feces in South Korea, and 146 were confirmed to be resistant to tetracycline. The tetracycline resistance genetet(A) (46.5%) was the most prevalent, followed bytet(B) (45.1%) andtet(C) (5.8%). Strains carryingtet(A) plustet(B) andtet(B) plustet(C) were detected in two isolates each. In terms of phylogenetic grouping, 101 (65.2%) isolates were classified as phylogenetic group B1, followed in decreasing order by D (17.4%), A (14.2%), and B2 (3.2%). Ninety-one (62.3%) isolates were determined to be multidrug resistant by the disk diffusion method. MIC testing using the principal tetracyclines, namely, tetracycline, chlortetracycline, oxytetracycline, doxycycline, and minocycline, revealed that isolates carryingtet(B) had higher MIC values than isolates carryingtet(A). Conjugation assays showed that 121 (82.9%) isolates could transfer a tetracycline resistance gene to a recipient via the IncFIB replicon (65.1%). This study suggests that the high prevalence of tetracycline-resistantE. coliisolates in beef cattle is due to the transferability of tetracycline resistance genes betweenE. colipopulations which have survived the selective pressure caused by the use of antimicrobial agents.

scholarly journals Transfer of CRISPR-like activity of MIMIVIRE into Bacteria

2019 ◽  
Author(s):  
Azza Said ◽  
La Scola Bernard ◽  
Levasseur Anthony ◽  
Perrin Pierre ◽  
Chabrière Eric ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Unknown Function ◽  
Tetracycline Resistance ◽  
Tetracycline Resistance Gene ◽  
Chloramphenicol Resistance ◽  
E Coli ◽  
Defence System

AbstractMIMIVIRE is a defence system utilized by lineage A Mimiviruses against Zamilon virophages. It is composed of a helicase, a nuclease and a gene of unknown function here named trcg (for Target Repeat-Containing gene), which contains four 15-bp repeats identical to the Zamilon sequence. Their silencing restored susceptibility to Zamilon, and the CRISPR-Cas4-like activity of the nuclease was recently characterised. We expressed these 3 genes after transformation of a modified strain of Escherichia coli made resistant to ampicillin, chloramphenicol and tetracycline. The virophage repeats were replaced with four repeats of 15 nucleotides identical to a sequence in the tetracycline resistance gene. The induction of the MIMIVIRE genes restored E. coli sensitivity to tetracycline; the tetracycline operon and its supporting plasmid harbouring the chloramphenicol resistance gene vanished. We therefore efficiently transferred the defence system MIMIVIRE from giant Mimivirus against virophage to E. coli to clear it from a plasmid.

scholarly journals Detection and linkage to mobile genetic elements of tetracycline resistance gene tet(M) in Escherichia coli isolates from pigs

2014 ◽  
Vol 10 (1) ◽  
pp. 155 ◽  
Author(s):  
Sonia Jurado-Rabadán ◽  
Ricardo de la Fuente ◽  
José A Ruiz-Santa-Quiteria ◽  
José A Orden ◽  
Lisbeth E de Vries ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Tetracycline Resistance ◽  
Mobile Genetic Elements ◽  
Tetracycline Resistance Gene ◽  
Genetic Elements
Sign in / Sign up

Export Citation Format

Share Document