The expression of parathyroid hormone-related protein (PTHrP) in normal parathyroid: histochemistry and in situ hybridization

1992 ◽  
Vol 98 (4) ◽  
pp. 211-215 ◽  
Author(s):  
Riko Kitazawa ◽  
Sohei Kitazawa ◽  
Masaaki Fukase ◽  
Takuo Fujita ◽  
Akira Kobayashi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Parathyroid Hormone ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein

Localization of parathyroid hormone-related protein in osteoclasts by in situ hybridization and immunohistochemistry

Bone ◽  
1998 ◽  
Vol 22 (3) ◽  
pp. 189-194 ◽  
Author(s):  
V. Kartsogiannis ◽  
N. Udagawa ◽  
K.W. Ng ◽  
T.J. Martin ◽  
J.M. Moseley ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Parathyroid Hormone ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein

Localization of parathyroid hormone-related protein mrna expression in breast cancer and metastatic lesions by in situ hybridization

2009 ◽  
Vol 7 (8) ◽  
pp. 971-979 ◽  
Author(s):  
Socorro J. Vargas ◽  
Matthew T. Gillespie ◽  
Gerard J. Powell ◽  
Justine Southby ◽  
Janine A. Danks ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Situ Hybridization ◽  
Parathyroid Hormone ◽  
Mrna Expression ◽  
Related Protein ◽  
Metastatic Lesions ◽  
Parathyroid Hormone Related Protein

scholarly journals In situ hybridization of parathyroid hormone-related protein in normal skin, skin tumors, and gynecological cancers using digoxigenin-labeled probes and antibody enhancement.

1995 ◽  
Vol 43 (1) ◽  
pp. 5-10 ◽  
Author(s):  
J A Danks ◽  
J C McHale ◽  
S P Clark ◽  
S T Chou ◽  
J P Scurry ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Parathyroid Hormone ◽  
Mrna Expression ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Oligonucleotide Probes ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein ◽  
Basal Cell Carcinomas

We describe a novel procedure for in situ hybridization that combines the use of digoxigenin-labeled oligonucleotide probes with an antibody enhancement step that can be performed on formalin-fixed, paraffin-embedded tissues. Addition of a second antibody enhances the visibility of parathyroid hormone-related protein (PTHrP) mRNA expression from barely to highly discernible and interpretable, with virtually no nonspecific background expression. This technique has allowed visualization of PTHrP mRNA in normal human skin and epithelium-derived tumors. PTHrP mRNA expression was confined to the basal and spinous keratinocyte layers of skin. There was strong hybridization in the spinous keratinocyte layer and a low level of hybridization in the basal layer. An extensive panel of positive and negative controls included poly d(T) probe to indicate total mRNA present in the sections. Squamous cell carcinomas and basal cell carcinomas of the skin, from pathology archives, were examined for the presence of PTHrP mRNA. The results reflected previous immunohistochemical studies, with every squamous cell carcinoma hybridizing strongly with the PTHrP probes. The basal cell carcinomas showed no expression of PTHrP mRNA, although the total mRNA signal was very strong. The localization of PTHrP mRNA in the tumors of the gynecological tract also reflected the immunohistochemical findings, with expression found in the squamous cell carcinomas but not in the adenocarcinomas. In situ hybridization with digoxigenin-labeled oligonucleotide probes and antibody enhancement has provided a sensitive, highly specific procedure for detection of PTHrP mRNA in tumors and normal tissue.

Effects of two synthetic parathyroid hormone-related protein fragments on maternofetal transfer of calcium and magnesium and release of cyclic AMP by the in-situ perfused rat placenta

1991 ◽  
Vol 129 (3) ◽  
pp. 399-404 ◽  
Author(s):  
A. J. Shaw ◽  
M. Z. Mughal ◽  
M. J. A. Maresh ◽  
C. P. Sibley
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Ringer Solution ◽  
Related Protein ◽  
Placental Perfusion ◽  
Protein Fragments ◽  
Parathyroid Hormone Related Protein ◽  
Peak Release ◽  
Similar Peak

ABSTRACT Two human parathyroid hormone-related protein (hPTHrP) fragments were tested for effects on maternofetal transfer of 45Ca and Mg across the in-situ perfused rat placenta at 21 days of gestation (term = 23 days). The fetal placental circulation was perfused with a Mg-free Krebs–Ringer solution and the unidirectional maternofetal clearance (Kmf) of 45Ca and Mg compared with that of 51Cr-EDTA, the latter being employed as a paracellular diffusional marker. Placental perfusion with hPTHrP(1–34) (100 ng/ml) or hPTHrP(75–86)amide (50 ng/ml) did not significantly alter the Kmf of 45Ca or that of Mg. In separate rats, however, hPTHrP(1–34) but not hPTHrP(75–86)amide stimulated marked placental cyclic AMP (cAMP) release, the peak response of 63±7 pmol/min occurring 10 min after the beginning of the peptide perfusion. A lower dose of hPTHrP(1–34) (4 ng/ml) produced a similar peak release of cAMP, as did [Nle8,21,Tyr34]-rPTH(1–34)amide (4 ng/ml) and the adenylate cyclase agonist forskolin (17 μmol/l). Forskolin also rapidly increased the Kmf of 45Ca but not that of Mg or 51Cr-EDTA. The present study indicates that hPTHrP does not acutely affect maternofetal transfer of Ca or Mg across the perfused rat placenta. The data also question the role played by cAMP in the stimulatory actions of forskolin on placental Ca transport. Journal of Endocrinology (1991) 129, 399–404

In situ demonstration of parathyroid hormone-related protein mRNA in sclerosing hepatic carcinoma

1999 ◽  
Vol 435 (2) ◽  
pp. 137-142 ◽  
Author(s):  
R. Kitazawa ◽  
Sohei Kitazawa ◽  
Seitetsu Yoon ◽  
Masato Kasuga ◽  
Sakan Maeda
Keyword(s):  
Parathyroid Hormone ◽  
Related Protein ◽  
Hepatic Carcinoma ◽  
Parathyroid Hormone Related Protein

Parathyroid Hormone–Related Protein Protects against Mammary Tumor Emergence and Is Associated with Monocyte Infiltration in Ductal Carcinoma In situ

2009 ◽  
Vol 69 (18) ◽  
pp. 7473-7479 ◽  
Author(s):  
Nicholas I. Fleming ◽  
Melanie K. Trivett ◽  
Joshy George ◽  
John L. Slavin ◽  
William K. Murray ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Mammary Tumor ◽  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein

Expression of parathyroid hormone-related protein mRNA in the rat before birth: demonstration by hybridization histochemistry

1991 ◽  
Vol 6 (3) ◽  
pp. 281-290 ◽  
Author(s):  
P. V. Senior ◽  
D. A. Heath ◽  
F. Beck
Keyword(s):  
Gene Expression ◽  
Parathyroid Hormone ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Giant Cells ◽  
Related Protein ◽  
Hybridization Histochemistry ◽  
Embryonic Tissues ◽  
Parathyroid Hormone Related Protein

ABSTRACT The ontogeny of parathyroid hormone (PTH) and PTH-related protein (PTHrP) gene expression was studied by hybridization histochemistry in the rat at various stages between implantation and full term. PTHrP mRNA was demonstrable in the early post-implantation trophoblastic giant cells but disappeared from this site before 13·5 days. Localized gene expression, detectable by the in-situ technique, began between 12·5 and 15·5 days in embryonic tissues. The distribution of gene expression suggests that PTHrP may be concerned with the process of implantation. Its widespread, yet clearly localized, distribution in embryonic and fetal tissues is consistent with a paracrine or autocrine function which may relate to the transforming growth factor-β family of growth factors. PTH expression occurred solely in the parathyroid and was detectable in the fetal parathyroid at 13·5 days of gestation.

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