Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea

1993 ◽  
Vol 236-236 (2-3) ◽  
pp. 369-373 ◽  
Author(s):  
Douglas C. Boyes ◽  
June B. Nasrallah
Keyword(s):  
Brassica Oleracea ◽  
The Self ◽  
Self Incompatibility ◽  
Physical Linkage ◽  
Incompatibility Locus

scholarly journals Location of the Self-Incompatibility Locus in an RFLP and RAPD Map of Brassica oleracea

1997 ◽  
Vol 88 (1) ◽  
pp. 57-60 ◽  
Author(s):  
L. E. A. Camargo ◽  
L. Savides ◽  
G. Jung ◽  
J. Nienhuis ◽  
T. C. Osborn
Keyword(s):  
Brassica Oleracea ◽  
The Self ◽  
Self Incompatibility ◽  
Incompatibility Locus

Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.

2019 ◽  
Vol 51 (7) ◽  
pp. 723-733 ◽  
Author(s):  
Songmei Shi ◽  
Qiguo Gao ◽  
Tonghong Zuo ◽  
Zhenze Lei ◽  
Quanming Pu ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Signaling Pathway ◽  
Chromosomal Region ◽  
The Self ◽  
Mature Stage ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Phylogenic Tree ◽  
Brassica Oleracea L

Abstract Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway.

scholarly journals Balancing selection and the crossing of fitness valleys in structured populations: diversification in the gametophytic self-incompatibility system

2021 ◽  
Author(s):  
Roman Stetsenko ◽  
Thomas Brom ◽  
Vincent Castric ◽  
Sylvain Billiard
Keyword(s):  
Population Structure ◽  
Allelic Diversity ◽  
Structured Populations ◽  
The Self ◽  
Population Subdivision ◽  
Island Population ◽  
Single Mutation ◽  
Self Incompatibility ◽  
S Locus ◽  
Incompatibility Locus

The self-incompatibility locus (S-locus) of flowering plants displays a striking allelic diversity. How such a diversity has emerged remains unclear. In this paper, we performed numerical simulations in a finite island population genetics model to investigate how population subdivision affects the diversification process at a S-locus, given that the two-genes architecture typical of S-loci involves the crossing of a fitness valley. We show that population structure increases the number of self-incompatibility haplotypes (S-haplotypes) maintained in the whole metapopulation, but at the same time also slightly reduces the parameter range allowing for their diversification. This increase is partly due to a reinforcement of the diversification and replacement dynamics of S-haplotypes within and among demes. We also show that the two-genes architecture leads to a higher diversity compared with a simpler genetic architecture where new S-haplotypes appear in a single mutation step. We conclude that population structure helps explain the large allelic diversity at the self-incompatibility locus. Overall, our results suggest that population subdivision can act in two opposite directions: it makes easier S-haplotypes diversification but increases the risk that the SI system is lost.

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