Towards an in vitro bioassay for the self-incompatibility response in Brassica oleracea

1989 ◽  
Vol 2 (4) ◽  
pp. 277-280 ◽  
Author(s):  
A. Singh ◽  
D. J. Paolillo
Keyword(s):  
Brassica Oleracea ◽  
The Self ◽  
Self Incompatibility ◽  
In Vitro Bioassay

An in vitro bioassay for the stigmatic product of the self-incompatibility gene in Papaver rhoeas L.

1988 ◽  
Vol 110 (1) ◽  
pp. 109-118 ◽  
Author(s):  
V. E. FRANKLIN-TONG ◽  
M. J. LAWRENCE ◽  
F. C. H. FRANKLIN
Keyword(s):  
The Self ◽  
Self Incompatibility ◽  
In Vitro Bioassay ◽  
Papaver Rhoeas

scholarly journals Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes.

1991 ◽  
pp. 271-283 ◽  
Author(s):  
J. E. Gray ◽  
B. A. McClure ◽  
I. Bonig ◽  
M. A. Anderson ◽  
A. E. Clarke
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Nicotiana Alata ◽  
Self Incompatibility

Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.

2019 ◽  
Vol 51 (7) ◽  
pp. 723-733 ◽  
Author(s):  
Songmei Shi ◽  
Qiguo Gao ◽  
Tonghong Zuo ◽  
Zhenze Lei ◽  
Quanming Pu ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Signaling Pathway ◽  
Chromosomal Region ◽  
The Self ◽  
Mature Stage ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Phylogenic Tree ◽  
Brassica Oleracea L

Abstract Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway.

scholarly journals A functional S locus anther gene is not required for the self-incompatibility response in Brassica oleracea.

1997 ◽  
Vol 9 (11) ◽  
pp. 2065-2076 ◽  
Author(s):  
M Pastuglia ◽  
V Ruffio-Châble ◽  
V Delorme ◽  
T Gaude ◽  
C Dumas ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
The Self ◽  
Self Incompatibility ◽  
S Locus

scholarly journals Isolation of a second S-locus-related cDNA from Brassica oleracea: genetic relationships between the S locus and two related loci.

Genetics ◽  
1991 ◽  
Vol 127 (1) ◽  
pp. 221-228 ◽  
Author(s):  
D C Boyes ◽  
C H Chen ◽  
T Tantikanjana ◽  
J J Esch ◽  
J B Nasrallah
Keyword(s):  
Brassica Oleracea ◽  
Fragment Length Polymorphism ◽  
Genetic Relationships ◽  
The Self ◽  
Self Incompatibility ◽  
S Locus ◽  
Related Sequence ◽  
Slg Gene ◽  
S Locus Glycoprotein

Abstract Self-incompatibility in Brassica oleracea is controlled by the highly polymorphic S locus. Isolation and subsequent characterization of the S-locus-glycoprotein (SLG) gene, which encodes the S-locus-specific glycoprotein (SLSG), has revealed the presence of a self-incompatibility multigene family. One of these S-locus-related genes, SLR1, has been shown to be expressed. In this study we present the isolation and preliminary characterization of a second expressed S-locus-related sequence, SLR2. Through restriction fragment length polymorphism (RFLP) linkage analysis we demonstrate that the SLR1 and SLR2 loci reside approximately 18.5 map units apart in one linkage group that segregates independently of the S-locus. The identification of a second SLR gene expressed in stigmas suggests that loci unlinked to the S-locus may play a role in the self-incompatibility response, or in pollination in general.

Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes

1991 ◽  
Vol 3 (3) ◽  
pp. 271 ◽  
Author(s):  
Julie E. Gray ◽  
Bruce A. McClure ◽  
Ingrid Bonig ◽  
Marilyn A. Anderson ◽  
Adrienne E. Clarke
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Nicotiana Alata ◽  
Self Incompatibility

Variability of the self-incompatibility reaction in Brassica oleracea L. with S 15 haplotype

2009 ◽  
Vol 23 (2) ◽  
pp. 141-151 ◽  
Author(s):  
Houria Hadj-Arab ◽  
Anne-Marie Chèvre ◽  
Thierry Gaude ◽  
Véronique Chable
Keyword(s):  
Brassica Oleracea ◽  
The Self ◽  
Self Incompatibility ◽  
Incompatibility Reaction ◽  
Brassica Oleracea L
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