Variation for 2n pollen production in clones of Solanum phureja Juz. and Buk.

1981 ◽  
Vol 59 (2) ◽  
pp. 95-100 ◽  
Author(s):  
R. E. Veilleux ◽  
F. I. Lauer
Keyword(s):  
Solanum Phureja ◽  
Pollen Production ◽  
2N Pollen

2n GAMETES IN DIPLOID SOLANUM: FREQUENCY AND TYPES OF SPINDLE ABNORMALITIES

1982 ◽  
Vol 24 (3) ◽  
pp. 301-314 ◽  
Author(s):  
R. E. Veilleux ◽  
N. A. McHale ◽  
F. I. Lauer
Keyword(s):  
Cross Sections ◽  
Meiotic Division ◽  
2N Gametes ◽  
Anatomical Study ◽  
Solanum Phureja ◽  
Spindle Orientation ◽  
Pollen Production ◽  
2N Pollen ◽  
Genetic Theory ◽  
Parallel Spindles

Cytological disturbances resulting in 2n pollen were examined in four clones of diploid (2x = 24) Solanum phureja Juz. and Buk.; two (148-17 and 127-14) were variable and two (154-1 and B2-11) were consistently high in 2n pollen production. Three types of abnormal spindle orientation were observed at the second meiotic division: fused, tripolar, and parallel spindles (fs, tps, and ps, respectively). All four genotypes had fs. Three had tps, and ps was least frequent, occuring in two genotypes only. Cross-sections of buds revealed that all three abnormalities can occur in adjacent cells of a locule. In all genotypes, fs predominated at high levels of expression of 2n gametes. At lower levels of expression (i.e. less than 60%, which occurred only in 148-17 and 127-14), however, the frequencies of tps and ps increased. In general, there was little evidence for localization of dyads (resulting from fs or ps) or tetrads (resulting from normal divisions) within cross sections of locules, but 52.4% of all tetrads which occurred in a single locule of genotype 127-14 were found in 25% of the locular area. Cross-sections of buds revealed variation in frequency of 2n gametes among anthers (61.7 vs. 5.6% in 148-17) and between locules of an anther (73.1 vs. 90.0% in B2-11). Implications of the anatomical study were applied to current genetic theory regarding the development of 2n gametes in potato species.

Genomic composition of 4x–2x potato hybrids: influence of Solanum chacoense

Genome ◽  
1991 ◽  
Vol 34 (3) ◽  
pp. 413-420 ◽  
Author(s):  
Fouzia Bani-Aameur ◽  
F. I. Lauer ◽  
R. E. Veilleux ◽  
A. Hilali
Keyword(s):  
Solanum Tuberosum ◽  
Interspecific Hybrids ◽  
Tuber Yield ◽  
Solanum Chacoense ◽  
Solanum Phureja ◽  
2N Pollen ◽  
Fall Season ◽  
Tuber Set ◽  
Target Environment ◽  
Breeding Selections

Tetraploid potato hybrids representing three genomic compositions (TTTT, TTPP, and TTPC (T, Solanum tuberosum; P, Solanum phureja; C, Solanum chacoense)) were constructed by crossing S. tuberosum (2n = 4x = 48) cultivars or advanced breeding selections among themselves or with 2n pollen producing selections of either S. phureja (2n = 2x = 24) or F1 hybrids between S. phureja and S. chacoense (2n = 2x = 24). Seedling families were evaluated in the fall season and selections within each family were evaluated in spring trials at Ait-Melloul, Morocco. Genomic composition was the greatest source of variance for yield-attributing traits, with TTPC outperforming other hybrids in fall trials for vine vigor, tuber set, and tuber yield and TTPP outperforming other hybrids in spring trials for the same traits. The results imply that analytic breeding for potato by construction of complex interspecific hybrids may result in clones surpassing a previously proposed heterotic threshold if the target environment is conducive to expression of favorable traits from alien germ plasm.Key words: Solanum phureja, Solanum chacoense, Solanum tuberosum, 4x–2x hybrids, potato, analytic breeding.

Variability of flowering and 2n pollen production in diploid potatoes under high temperatures

1987 ◽  
Vol 64 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Kathleen G. Haynes ◽  
F. L. Haynes ◽  
William H. Swallow
Keyword(s):  
High Temperatures ◽  
Pollen Production ◽  
2N Pollen

Use of Ipomoea trifida (HBK.) G. Don germ plasm for sweet-potato improvement. 2. Fertility of synthetic hexaploids and triploids with 2n gametes of I. trifida, and their interspecific crossability with sweet potato

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 209-214 ◽  
Author(s):  
Rosanna Freyre ◽  
Masaru Iwanaga ◽  
Gisella Orjeda
Keyword(s):  
Sweet Potato ◽  
Egg Production ◽  
Ipomoea Batatas ◽  
2N Gametes ◽  
Pollen Production ◽  
2N Pollen ◽  
Ipomoea Trifida ◽  
Potato Improvement ◽  
Synthetic Hexaploids ◽  
Interspecific Crossability

Twenty-two synthetic hexaploids and 33 triploids with 2n pollen production of Ipomoea trifida were used in crosses to estimate their male and female fertility and their crossability with sweet potato, Ipomoea batatas (L.) Lam. Several important conclusions were drawn. (i) The fertility of pollen of the triploid plants was confirmed, which suggested they are truly functional 2n pollen. (ii) The average male fertility of hexaploid plants was higher than that of triploid with 2n pollen production plants. (iii) 2n egg production was observed in several triploid genotypes, (iv) From a polycross, 3079 seeds with normal shapes were produced. These represent a population of hexaploid I. trifida with a wide genetic base, and they may be important material for sweet-potato improvement, (v) A total of 3275 seeds were obtained from crosses with six sweet-potato female parents, of which only 60 germinated, indicating the existence of an interspecific barrier. The implications of 2n gametes for breeding and evolution of sweet potato are also discussed.Key words: Ipomoea batatas, Ipomoea trifida, 2n gametes, sexual polyploidization, triploid.

Anther culture of a 2n pollen producing clone of Solanum phureja Juz. &Buk.

1985 ◽  
Vol 27 (5) ◽  
pp. 559-564 ◽  
Author(s):  
R. E. Veilleux ◽  
J. Booze-Daniels ◽  
E. Pehu
Keyword(s):  
Anther Culture ◽  
Polyacrylamide Gel Electrophoresis ◽  
Pollen Grains ◽  
Unreduced Gametes ◽  
Solanum Phureja ◽  
2N Pollen ◽  
Regenerated Plants ◽  
Series Of Experiments ◽  
Pollen Formation ◽  
First And Second Generation

In a series of experiments, a total of 95 plants were regenerated from culture of 1416 anthers of a single genotype (PP5) of Solanum phureja that expressed a variable frequency of 2n pollen by the genetic equivalent of first division restitution. The regenerated plants included 29 monoploids (2n = x = 12), 58 diploids (2n = 2x = 24) and 8 tetraploids (2n = 4x = 48). Monoploids carrying the potential for 2n pollen formation are central to the development of a breeding scheme to construct highly heterozygous diplandroids. Segregation of tuber flesh color and tuber protein bands revealed by polyacrylamide gel electrophoresis in first and second generation anther-derived diploid plants provided evidence for both embryogenesis of 2n pollen as well as doubling of monoploid genomes as sources of anther-derived diploids. Because of variation for the same genetic markers in anther-derived tetraploids, sources other than embryogenesis of doubly restituted (4n) pollen grains were implied. An enhanced response to anther culture was noted in some anther-derived dihaploids.Key words: unreduced gametes, potato, anther culture, diplandroid, monoploid.

scholarly journals Effects of Colchicine on Populus canescens Ectexine Structure and 2n Pollen Production

2020 ◽  
Vol 11 ◽  
Author(s):  
Qing Zhou ◽  
Jian Wu ◽  
Yaru Sang ◽  
Zhengyang Zhao ◽  
Pingdong Zhang ◽  
...  
Keyword(s):  
Pollen Production ◽  
2N Pollen

Male fertility and 2n pollen production in haploid-wild species hybrids

1985 ◽  
Vol 62 (9) ◽  
pp. 479-487 ◽  
Author(s):  
Shelley A. Hermundstad ◽  
S. J. Peloquin
Keyword(s):  
Wild Species ◽  
Male Fertility ◽  
Pollen Production ◽  
2N Pollen ◽  
Species Hybrids

Discrepancy between spindle anomalies and 2N pollen production insolanum interspecific hybrids

1997 ◽  
Vol 74 (6) ◽  
pp. 399-403 ◽  
Author(s):  
Amalia Barone ◽  
Domenico Carputo ◽  
Giuliana La Rotonda ◽  
Luigi Frusciante
Keyword(s):  
Interspecific Hybrids ◽  
Pollen Production ◽  
2N Pollen

Use of Ipomoea trifida (HBK.) G. Don germ plasm for sweet potato improvement. 1. Development of synthetic hexaploids of I. trifida by ploidy-level manipulations

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Masaru Iwanaga ◽  
Rosanna Freyre ◽  
Gisella Orjeda
Keyword(s):  
Sweet Potato ◽  
Ploidy Level ◽  
Ipomoea Batatas ◽  
Colchicine Treatment ◽  
Pollen Production ◽  
2N Pollen ◽  
Ipomoea Trifida ◽  
Pollen Sample ◽  
Colchicine Solution ◽  
Synthetic Hexaploids

Crosses were made between 21 tetraploid accessions and 41 diploid accessions of Ipomoea trifida, obtaining a total of 9185 triploid seeds from 215 different cross combinations. Doubling the somatic chromosome number of the triploids was attempted by colchicine treatment on young seedlings to obtain synthetic hexaploids. A total of 787 axillary buds of 316 triploid plants were treated with a 0.5% colchicine solution for 24 h, applied to cotton plugs surrounding the buds. The survival rate of the treated buds was, on average, 41%. The ploidy level in germ-layer L2 was determined in 258 clones by evaluating a pollen sample from each clone. Fifty-five clones were selected for high stainability and pollen size. The selected genotypes were meiotically analyzed. Twenty-two of them were identified as hexaploid and 33 as triploid with 2n pollen production. The present study is the first report on 2n pollen production in triploid plants of Ipomoea species. The use of these triploid clones with 2n pollen production and synthetic hexaploid clones for sweet-potato breeding is discussed.Key words: Ipomoea batatas, Ipomoea trifida, colchicine, triploids, 2n pollen.

scholarly journals Unreduced 2n Pollen Production in ‘Nishiuchi Konatsu’ Hyuganatsu as Inferred by Pollen Characteristics and Progeny Ploidy Level

2012 ◽  
Vol 81 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Chitose Honsho ◽  
Eri Yamamura ◽  
Kyoko Tsuruta ◽  
Yukako Yoshimaru ◽  
Kiichi Yasuda ◽  
...  
Keyword(s):  
Ploidy Level ◽  
Pollen Production ◽  
2N Pollen
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