Genomic composition of 4x–2x potato hybrids: influence of Solanum chacoense

Genome ◽  
1991 ◽  
Vol 34 (3) ◽  
pp. 413-420 ◽  
Author(s):  
Fouzia Bani-Aameur ◽  
F. I. Lauer ◽  
R. E. Veilleux ◽  
A. Hilali

Tetraploid potato hybrids representing three genomic compositions (TTTT, TTPP, and TTPC (T, Solanum tuberosum; P, Solanum phureja; C, Solanum chacoense)) were constructed by crossing S. tuberosum (2n = 4x = 48) cultivars or advanced breeding selections among themselves or with 2n pollen producing selections of either S. phureja (2n = 2x = 24) or F1 hybrids between S. phureja and S. chacoense (2n = 2x = 24). Seedling families were evaluated in the fall season and selections within each family were evaluated in spring trials at Ait-Melloul, Morocco. Genomic composition was the greatest source of variance for yield-attributing traits, with TTPC outperforming other hybrids in fall trials for vine vigor, tuber set, and tuber yield and TTPP outperforming other hybrids in spring trials for the same traits. The results imply that analytic breeding for potato by construction of complex interspecific hybrids may result in clones surpassing a previously proposed heterotic threshold if the target environment is conducive to expression of favorable traits from alien germ plasm.Key words: Solanum phureja, Solanum chacoense, Solanum tuberosum, 4x–2x hybrids, potato, analytic breeding.

Botany ◽  
2011 ◽  
Vol 89 (5) ◽  
pp. 289-299 ◽  
Author(s):  
Marie-Claude Moisan ◽  
Jean Rivoal

We have developed an extraction procedure that improves the stability of potato ( Solanum tuberosum L.) tuber hexokinase (HK) after extraction. Using this protocol, we showed that at least four HK isoforms are present in this tissue, and they can be separated by hydrophobic-interaction chromatography on a butyl-Sepharose™ 4 Fast Flow column. One of the main HK isoforms was purified to homogeneity using further chromatographic separations on red dye, DEAE Fractogel, hydroxyapatite, cibacron blue, and MonoQ matrices. HK-specific activity of this fraction (10.2 U·mg protein–1) corresponds to an enrichment of more than 5500-fold, with a yield of 0.9%. This is the highest reported HK-specific activity from a plant source. The purified enzyme consisted of a monomer with a subunit apparent Mr of 51 kDa when analyzed by SDS–PAGE. This polypeptide was recognized by affinity-purified anti- Solanum chacoense Bitt. recombinant HK IgGs. The protein was digested with trypsin and its digestion products were subjected to MS – MS sequencing after HPLC separation. The sequences of these tryptic peptides matched the predicted coding sequence of the S. tuberosum HK1 gene with a coverage of 57%. Examination of the kinetic properties of the purified protein HK1 indicates that it may be regulated by the internal O2 concentration of the tuber because of its sensitivity to acidic pHs and inhibition by ADP.


2018 ◽  
Vol 10 (28) ◽  
pp. 50-63
Author(s):  
mina moghaddaszadeh ◽  
Rasool Asghari Zakaria ◽  
Davoud Hassanpanah ◽  
naser zare ◽  
◽  
...  

2020 ◽  
Vol 1 (1) ◽  
pp. p39
Author(s):  
Nyasha Sakadzo ◽  
Fortune Tafirenyika ◽  
Kasirayi Makaza

Potato (Solanum tuberosum L) is a strategic national food security crop in that can be used as a supplement for carbohydrates. It is the fastest growing staple food crop and source of income for poor smallholder farmers. There is limited documentation on effects of earthing up irish potatoes on yield and yield components in Zimbabwe. A field experiment was conducted in Zaka district of Masvingo province in Zimbabwe during the 2018/19 cropping season. The aim of the study was to investigate the effect of time of earthing up on yield and yield parameters of BP1 potato variety. The treatments consisted of four levels of time of earthing up (no earthing up (control), three, four and five weeks after plant emergence), replicated three times on a Randomized Complete Block Design (RCBD). Results on earthing-up at three weeks recorded significantly (P<0.013) the highest total tuber yield (21.97 t ha-1) which is 10% higher compared to no earthing up which recorded 14.43 t ha-1. Moreover, earthing up at two weeks had the least number of greening tuber yield (3%), 11% pest damaged tuber yield, 6% unmarketable tuber yield and 45% marketable tuber yield. This is in contrast with no earthing up which recorded 91% greening tuber yield, 25.3% on pest damaged tubers, 59% on unmarketable tubers and 10% marketable tuber yield. Based on the results, first earthing up should be done at three weeks after complete plant emergence at the study area under rain fed conditions for the production of potatoes with improved yield and better tuber quality.


2016 ◽  
Vol 9 (1) ◽  
pp. 156-170
Author(s):  
Juan Bautista López ◽  
María Elena Márquez ◽  
Sonia Jaramillo ◽  
José Luís Zapata

El problema más limitante del cultivo de la papa en muchas regiones del mundo, lo constituye la gota o tizón tardío ocasionado por el hongo Phytophthora infestans. Esta investigación se realizó con el objetivo de determinar variaciones poblacionales de este patógeno, basadas en el tipo de apareamiento y en la presencia de razas fisiológicas en 19 aislamientos de P. infestans obtenidos en plantas de papa (Solanum tuberosum) variedades Diacol Capiro, ICA Puracé e ICA Cumanday; papa criolla (Solanum phureja), pepino (Solanum muricatum) y tomate (Lycopersicon esculentum) en diferentes zonas del Departamento de Antioquia (Colombia).Los resultados obtenidos a partir de un apareamiento patrón A1, con los aislamientos colectados en las diferentes zonas sugieren la existencia de una población de P. infestans con un solo tipo de apareamiento. La prueba de virulencia del hongo, realizada en folíolos de 11 diferenciales de papa mostró la presencia de 6 razas fisiológicas, de las cuales la más frecuente fue la 7446 (58%), con los factores de virulencia 1-2-3-4-7-10-11. Aislamientos monozoospóricos presentaron los mismos factores de virulencia que sus respectivos aislamientos recolectados en el campo, lo que sugiere homogeneidad de la población fungosa. La gran complejidad de las razas fisiológicas de P. infestans podría estar influenciada por diversos factores genéticos y medioambientales.Aceptado para publicación: mayo 1997.


2007 ◽  
Vol 55 (3) ◽  
pp. 471-477 ◽  
Author(s):  
Elsa Lucila Camadro ◽  
Sandra Karina Saffarano ◽  
Juan Carlos Espinillo ◽  
Mateo Castro ◽  
Phillip W. Simon

1970 ◽  
Vol 38 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Semiha Güler

Effects of nitrogen on the leaf chlorophyll (chl), yield and yield attributing characters of potato as tuber number and mean tuber weight were studied. Correlation coefficients between the investigated characters were determined. Five nitrogen rates (0, 150, 200, 250 and 300 kg/ha) and four potato cultivars (Burren, Slaney, Anna and Emma) were used in the study. First, second, third class tuber yields and total tuber yield, tuber number per plant, and leaf chl were significantly influenced by both nitrogen rate and cultivar, whereas mean tuber weight was affected only by cultivar. Maximum total yield was obtained at 200 kg N/ha. There was significant linear relationship between leaf chl and N applied (R2=0.91). There were significant correlations between chl and yield and yield related characters. Total yield significantly correlated with leaf chl. Correlations between first class yield and total yield as well as total yield and tuber number per plant were highly significant.   Key words: Potato; Solanum tuberosum L.; Leaf chlorophyll; Nitrogen; Yield DOI: 10.3329/bjb.v38i2.5141 Bangladesh J. Bot. 38(2): 163-169, 2009 (December)  


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