Assessment of genomic and species relationships in Triticum and Aegilops by PAGE and by differential staining of seed albumins and globulins

1978 ◽  
Vol 52 (6) ◽  
pp. 273-280 ◽  
Author(s):  
K. A. Caldwell ◽  
D. D. Kasarda
Keyword(s):  
Differential Staining ◽  
Species Relationships ◽  
Seed Albumins

A Study of the Ultrastructure of Visual Cell Outer Segment Membranes, Using a Water-Miscible Embedding Medium and Differential Staining

1972 ◽  
Vol 30 ◽  
pp. 50-51
Author(s):  
Anthony J. Godfrey
Keyword(s):  
Outer Segment ◽  
Protein Denaturation ◽  
Uranyl Acetate ◽  
Differential Staining ◽  
Visual Cell ◽  
Lead Citrate ◽  
Chick Retina ◽  
Dark Staining ◽  
Embedding Medium ◽  
The Individual

Aldehyde-fixed chick retina was embedded in a water-containing resin of glutaraldehyde and urea, without dehydration. The loss of lipids and other soluble tissue components, which is severe in routine methods involving dehydration, was thereby minimized. Osmium tetroxide post-fixation was not used, lessening the amount of protein denaturation which occurred. Ultrathin sections were stained with 1, uranyl acetate and lead citrate, 2, silicotungstic acid, or 3, osmium vapor, prior to electron microscope examination of visual cell outer segment ultrastructure, at magnifications up to 800,000.Sections stained with uranyl acetate and lead citrate (Fig. 1) showed that the individual disc membranes consisted of a central lipid core about 78Å thick in which dark-staining 40Å masses appeared to be embedded from either side.

scholarly journals Chinese hamster ovary cell line DXB-11: chromosomal instability and karyotype heterogeneity

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Victoria I. Turilova ◽  
Tatyana S. Goryachaya ◽  
Tatiana K. Yakovleva
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Chromosome 8 ◽  
Ovary Cell ◽  
Differential Staining ◽  
Nucleolar Organizer Regions ◽  
Karyotype Heterogeneity

Abstract Background Chinese hamster ovary cell lines, also known as CHO cells, represent a large family of related, yet quite different, cell lines which are metabolic mutants derived from the original cell line, CHO-ori. Dihydrofolate reductase-deficient DXB-11 cell line, one of the first CHO derivatives, serves as the host cell line for the production of therapeutic proteins. It is generally assumed that DXB-11 is identical to DUKX or CHO-DUK cell lines, but, to our knowledge, DXB-11 karyotype has not been described yet. Results Using differential staining approaches (G-, C-banding and Ag-staining), we presented DXB-11 karyotype and revealed that karyotypes of DXB-11 and CHO-DUK cells have a number of differences. Although the number of chromosomes is equal—20 in each cell line—DXB-11 has normal chromosomes of the 1st and 5th pairs as well as an intact chromosome 8. Besides, in DXB-11 line, chromosome der(Z9) includes the material of chromosomes X and 6, whereas in CHO-DUK it results from the translocation of chromosomes 1 and 6. Ag-positive nucleolar organizer regions were revealed in the long arms of chromosome del(4)(q11q12) and both chromosome 5 homologues, as well as in the short arms of chromosomes 8 and add(8)(q11). Only 19 from 112 (16.96%) DXB-11 cells display identical chromosome complement accepted as the main structural variant of karyotype. The karyotype heterogeneity of all the rest of cells (93, 83.04%) occurs due to clonal and nonclonal additional structural rearrangements of chromosomes. Estimation of the frequency of chromosome involvement in these rearrangements allowed us to reveal that chromosomes 9, der(X)t(X;3;4), del(2)(p21p23), del(2)(q11q22) /Z2, der(4) /Z7, add(6)(p11) /Z8 are the most stable, whereas mar2, probably der(10), is the most unstable chromosome. A comparative analysis of our own and literary data on CHO karyotypes allowed to designate conservative chromosomes, both normal and rearranged, that remain unchanged in different CHO cell lines, as well as variable chromosomes that determine the individuality of karyotypes of CHO derivatives. Conclusion DXB-11and CHO-DUK cell lines differ in karyotypes. The revealed differential instability of DXB-11 chromosomes is likely not incidental and results in karyotype heterogeneity of cell population.

Mechanism of Differential Staining in Carious Dentin

1983 ◽  
Vol 62 (6) ◽  
pp. 713-714 ◽  
Author(s):  
Y. Kuboki ◽  
C.-F. Liu ◽  
T. Fusayama
Keyword(s):  
Lactic Acid ◽  
Differential Staining ◽  
Bacterial Origin ◽  
Dentin Matrix

The caries detector, 1.0% acid red sotution in proyylene glycol, failed to stain EDTA-demineralized bavine dentin matrix, but stained the same matrix when treated by lactic acid in a concentration above 0.01 M. Thus, acid of bacterial origin could possibly be responsible for the stainability change of carlous dentin in vivo.

scholarly journals SELECTIVE "STAINING" FOR ELECTRON MICROGRAPHY

1942 ◽  
Vol 76 (1) ◽  
pp. 103-108 ◽  
Author(s):  
Stuart Mudd ◽  
Thomas F. Anderson
Keyword(s):  
Heavy Metals ◽  
Cell Walls ◽  
Differential Staining ◽  
Bacterial Cells ◽  
Selective Staining ◽  
Electron Micrography ◽  
Chemical Effects ◽  
Microchemical Analysis ◽  
Cell Components ◽  
Selective Chemical

The physical basis of contrast and image formation in electron micrography is considered in relation to the possibility of recording selective chemical effects on cell components. A technology of selective microchemical analysis, equivalent to differential staining, is suggested as practicable in electron micrography. Electron pictures of bacteria after exposure to salts of heavy metals have shown the bacterial inner protoplasm, but not the cell walls, to be selectively darkened; shrinkage, coagulation, or escape of protoplasm from the injured cells may result and be recorded in the electron micrographs. Recording of the action of germicidal agents on individual bacterial cells is indicated as one promising field of application of microchemical analysis with the aid of the electron microscope.

scholarly journals Species Disjunctions in Larrea: Evidence From Morphology, Cytogenetics, Phenolic Compounds, and Seed Albumins

1972 ◽  
Vol 59 (2) ◽  
pp. 224 ◽  
Author(s):  
J. H. Hunziker ◽  
R. A. Palacios ◽  
Amalia G. De Valesi ◽  
Lidia Poggio
Keyword(s):  
Phenolic Compounds ◽  
Seed Albumins

scholarly journals The Differential Staining of Nucleated Blood Corpuscles

1882 ◽  
Vol 14 (345supp) ◽  
pp. 5510-5510
Author(s):  
Allen Y. Moore
Keyword(s):  
Differential Staining

scholarly journals Reconstructing Species Relationships within the Recently Diversified GenusOdontitesLudw. (Orobanchaceae): Evidence for Extensive Reticulate Evolution

2018 ◽  
Vol 179 (1) ◽  
pp. 1-20 ◽  
Author(s):  
Myriam Gaudeul ◽  
Sonja Siljak-Yakovlev ◽  
Tae-Soo Jang ◽  
Germinal Rouhan
Keyword(s):  
Reticulate Evolution ◽  
Species Relationships
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