Ultrastructural localization of pyruvate dehydrogenase in rat heart muscle

Histochemie ◽  
1973 ◽  
Vol 34 (4) ◽  
pp. 355-360 ◽  
Author(s):  
M. L. Nestorescu ◽  
E. A. Siess ◽  
O. H. Wieland
Keyword(s):  
Pyruvate Dehydrogenase ◽  
Heart Muscle ◽  
Rat Heart ◽  
Ultrastructural Localization

Interconversion of pyruvate dehydrogenase in rat heart muscle upon perfusion with fatty acids or ketone bodies

FEBS Letters ◽  
1971 ◽  
Vol 15 (4) ◽  
pp. 295-298 ◽  
Author(s):  
O. Wieland ◽  
H.v. Funcke ◽  
G. Löffler
Keyword(s):  
Fatty Acids ◽  
Pyruvate Dehydrogenase ◽  
Heart Muscle ◽  
Rat Heart ◽  
Ketone Bodies

Effects of aging on the activities of pyruvate dehydrogenase complex and its kinase in rat heart

Life Sciences ◽  
1997 ◽  
Vol 60 (25) ◽  
pp. 2309-2314 ◽  
Author(s):  
Naoya Nakai ◽  
Yuzo Sato ◽  
Yoshiharu Oshida ◽  
Atsushi Yoshimura ◽  
Noriaki Fujitsuka ◽  
...  
Keyword(s):  
Pyruvate Dehydrogenase ◽  
Rat Heart ◽  
Pyruvate Dehydrogenase Complex ◽  
Effects Of Aging ◽  
Dehydrogenase Complex

scholarly journals Starvation and Diabetes Reduce the Amount of Pyruvate Dehydrogenase Phosphatase in Rat Heart and Kidney

Diabetes ◽  
2003 ◽  
Vol 52 (6) ◽  
pp. 1371-1376 ◽  
Author(s):  
B. Huang ◽  
P. Wu ◽  
K. M. Popov ◽  
R. A. Harris
Keyword(s):  
Pyruvate Dehydrogenase ◽  
Rat Heart ◽  
Pyruvate Dehydrogenase Phosphatase

scholarly journals Conversion of inactive (phosphorylated) pyruvate dehydrogenase complex into active complex by the phosphate reaction in heart mitochondria is inhibited by alloxan-diabetes or starvation in the rat

1978 ◽  
Vol 173 (2) ◽  
pp. 669-680 ◽  
Author(s):  
N J Hutson ◽  
A L Kerbey ◽  
P J Randle ◽  
P H Sugden
Keyword(s):  
Pyruvate Dehydrogenase ◽  
Rat Heart ◽  
Pyruvate Dehydrogenase Complex ◽  
Diabetic Rats ◽  
Atp Depletion ◽  
Heart Mitochondria ◽  
Active Complex ◽  
Phosphate Complex ◽  
Rat Hearts ◽  
Dehydrogenase Complex

1. The conversion of inactive (phosphorylated) pyruvate dehydrogenase complex into active (dephosphorylated) complex by pyruvate dehydrogenase phosphate phosphatase is inhibited in heart mitochondria prepared from alloxan-diabetic or 48h-starved rats, in mitochondria prepared from acetate-perfused rat hearts and in mitochondria prepared from normal rat hearts incubated with respiratory substrates for 6 min (as compared with 1 min). 2. This conclusion is based on experiments with isolated intact mitochondria in which the pyruvate dehydrogenase kinase reaction was inhibited by pyruvate or ATP depletion (by using oligomycin and carbonyl cyanide m-chlorophenylhydrazone), and in experiments in which the rate of conversion of inactive complex into active complex by the phosphatase was measured in extracts of mitochondria. The inhibition of the phosphatase reaction was seen with constant concentrations of Ca2+ and Mg2+ (activators of the phosphatase). The phosphatase reaction in these mitochondrial extracts was not inhibited when an excess of exogenous pig heart pyruvate dehydrogenase phosphate was used as substrate. It is concluded that this inhibition is due to some factor(s) associated with the substrate (pyruvate dehydrogenase phosphate complex) and not to inhibition of the phosphatase as such. 3. This conclusion was verified by isolating pyruvate dehydrogenase phosphate complex, free of phosphatase, from hearts of control and diabetic rats an from heart mitochondria incubed for 1min (control) or 6min with respiratory substrates. The rates of re-activation of the inactive complexes were then measured with preparations of ox heart or rat heart phosphatase. The rates were lower (relative to controls) with inactive complex from hearts of diabetic rats or from heart mitochondria incubated for 6min with respiratory substrates. 4. The incorporation of 32Pi into inactive complex took 6min to complete in rat heart mitocondria. The extent of incorporation was consistent with three or four sites of phosphorylation in rat heart pyruvate dehydrogenase complex. 5. It is suggested that phosphorylation of sites additional to an inactivating site may inhibit the conversion of inactive complex into active complex by the phosphatase in heart mitochondria from alloxan-diabetic or 48h-starved rats or in mitochondria incubated for 6min with respiratory substrates.

Carbon Dioxide Fixation by Rat Heart Muscle in Vivo

1953 ◽  
Vol 173 (2) ◽  
pp. 335-336
Author(s):  
H. Eugene Hall ◽  
Edward W. Hawthorne ◽  
Lawrence M. Marshall
Keyword(s):  
Carbon Dioxide ◽  
Heart Muscle ◽  
Rat Heart ◽  
Carbon Dioxide Fixation

Changes of organic acids in rat heart muscle under ischemic-like conditions

1982 ◽  
Vol 227 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Shin-ichi Haraguchi ◽  
Hironori Toshima ◽  
Isamu Matsumoto ◽  
Tomiko Kuhara ◽  
Toshihiro Shinka
Keyword(s):  
Organic Acids ◽  
Heart Muscle ◽  
Rat Heart
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