Construction by one-step gene replacement of Trichoderma reesei strains that produce the glucoamylase P of Hormoconis resinae

1994 ◽  
Vol 26 (5-6) ◽  
pp. 422-429 ◽  
Author(s):  
Vesa V. Joutsjoki
Keyword(s):  
Trichoderma Reesei ◽  
Gene Replacement ◽  
Hormoconis Resinae ◽  
One Step

High frequency one-step gene replacement in Trichoderma reesei. II. Effects of deletions of individual cellulase genes

1993 ◽  
Vol 241-241 (5-6) ◽  
pp. 523-530 ◽  
Author(s):  
Pirkko L. Suominen ◽  
Arja L. Mäntylä ◽  
Taina Karhunen ◽  
Satu Hakola ◽  
Helena Nevalainen
Keyword(s):  
Trichoderma Reesei ◽  
High Frequency ◽  
Gene Replacement ◽  
One Step

High frequency one-step gene replacement in Trichoderma reesei. I. Endoglucanase I overproduction

1993 ◽  
Vol 241-241 (5-6) ◽  
pp. 515-522 ◽  
Author(s):  
Taina Karhunen ◽  
Arja Mäntylä ◽  
K. M. Helena Nevalainen ◽  
Pirkko L. Suominen
Keyword(s):  
Trichoderma Reesei ◽  
High Frequency ◽  
Gene Replacement ◽  
One Step ◽  
Endoglucanase I

Transformation of Trichoderma reesei with the Hormoconis resinae glucoamylase P (gamP) gene: production of a heterologous glucoamylase by Trichoderma reesei

1993 ◽  
Vol 24 (3) ◽  
pp. 223-228 ◽  
Author(s):  
Vesa V. Joutsjoki ◽  
Tuula K. Torkkeli ◽  
K. M. Helena Nevalainen
Keyword(s):  
Trichoderma Reesei ◽  
Hormoconis Resinae

scholarly journals Homologous recombination as the main mechanism for DNA integration and cause of rearrangements in the filamentous ascomycete Ashbya gossypii.

Genetics ◽  
1995 ◽  
Vol 140 (3) ◽  
pp. 973-987 ◽  
Author(s):  
S Steiner ◽  
J Wendland ◽  
M C Wright ◽  
P Philippsen
Keyword(s):  
Resistance Gene ◽  
Gene Replacement ◽  
Phytopathogenic Fungus ◽  
Ashbya Gossypii ◽  
Aminoglycoside Resistance ◽  
Genetic Changes ◽  
Apparent Lack ◽  
Dna Integration ◽  
Filamentous Ascomycete ◽  
One Step

Abstract A slow and a fast growth phenotype were observed after transformation of the phytopathogenic fungus Ashbya gossypii using a plasmid carrying homologous DNA and as selectable marker the Tn903 aminoglycoside resistance gene expressed from a strong A. gossypii promoter. Transformations with circular plasmids yielded slowly and irregularly growing geneticin-resistant mycelia in which 1% of nuclei contained plasmid sequences. Occasionally, fast growing sectors appeared which were shown to be initiated by homologous integration of the transforming DNA. Transformants obtained with plasmids linearized within the homology region immediately exhibited fast radial growth. In all 28 transformants analyzed plasmid DNA was integrated homologously. Such apparent lack of nonhomologous recombination has so far not been observed in filamentous ascomycetes. In 14 transformants two to four tandemly integrated plasmid copies were found. They underwent several types of genetic changes, mainly in the older mycelium: excision of whole plasmid copies and rearrangements within the integrated DNA (inversions and deletions). These internal rearrangements involved 360-bp inverted repeats, remnants of IS-elements flanking the resistance gene, and 156-bp direct repeats, originating from the strong A. gossypii promoter. Improved vectors lacking sequence repetitions were constructed and used for stable one-step gene replacement in A. gossypii.

4,6-Dimethoxy-1,3,5-triazine oligoxyloglucans: Novel one-step preparable substrates for studying action of endo-β-1,4-glucanase III from Trichoderma reesei

2010 ◽  
Vol 20 (12) ◽  
pp. 3588-3591 ◽  
Author(s):  
Atsushi Kobayashi ◽  
Tomonari Tanaka ◽  
Kazuhito Watanabe ◽  
Masaki Ishihara ◽  
Masato Noguchi ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
One Step

scholarly journals Novel Cellulase Profile of Trichoderma reesei Strains Constructed by cbh1 Gene Replacement with eg3 Gene Expression Cassette

2004 ◽  
Vol 36 (10) ◽  
pp. 667-672 ◽  
Author(s):  
Tian-Hong Wang ◽  
Ti Liu ◽  
Zhi-Hong Wu ◽  
Shi-Li Liu ◽  
Yi Lu ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Trichoderma Reesei ◽  
Filamentous Fungus ◽  
Gene Replacement ◽  
Industrial Applications ◽  
Dot Blot ◽  
Cellobiohydrolase I ◽  
Endoglucanase Activity ◽  
Coding Sequence ◽  
Gene Expression Cassette

Abstract To construct strains of the filamentous fungus Trichoderma reesei with low cellobiohydrolases while high endoglucanase activity, the Pcbh1-eg3-Tcbh1 cassette was constructed and the coding sequence of the cellobiohydrolase I (CBHI) gene was replaced with the coding sequence of the eg3 gene by homologous recombination. Disruption of the cbh1 gene was confirmed by PCR, Southern dot blot and Western hybridization analysis in two transformants denoted as L13 and L29. The filter paper-hydrolyzing activity of strain L29 was 60% of the parent strain Rut C30, and the CMCase activity was increased by 33%. This relatively modest increase suggested that the eg3 cDNA under the control of the cbh1 promoter was not efficiently transcribed as the wild type cbhl gene. However our results confirmed that homologous recombination could be used to construct strains of the filamentous fungus Trichoderma reesei with novel cellulase profile. Such strains are of interest from the basic science perspective and also have potential industrial applications.

A rapid, one step molecular identification of Trichoderma citrinoviride and Trichoderma reesei

2015 ◽  
Vol 31 (6) ◽  
pp. 995-999 ◽  
Author(s):  
Dina B. Saroj ◽  
Shrinivas N. Dengeti ◽  
Supriya Aher ◽  
Anil K. Gupta
Keyword(s):  
Trichoderma Reesei ◽  
Molecular Identification ◽  
One Step ◽  
Trichoderma Citrinoviride

One-step gene replacement in yeast by cotransformation

Gene ◽  
1985 ◽  
Vol 36 (1-2) ◽  
pp. 87-95 ◽  
Author(s):  
Hans Rudolph ◽  
Isabelle Koenig-Rauseo ◽  
Albert Hinnen
Keyword(s):  
Gene Replacement ◽  
One Step
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