Genetic control of plasmid DNA double-strand gap repair in yeast, Saccharomyces cerevisiae

1990 ◽  
Vol 18 (1) ◽  
pp. 1-5 ◽  
Author(s):  
V. M. Glaser ◽  
A. V. Glasunov ◽  
G. G. Tevzadze ◽  
J. R. Perera ◽  
S. V. Shestakov
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Control ◽  
Plasmid Dna ◽  
Yeast Saccharomyces Cerevisiae

Purification and genetic control of ?-pheromone-inactivating glycoproteins in the yeast Saccharomyces cerevisiae

1984 ◽  
Vol 9 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Hiroaki Fujimura ◽  
Naohiko Yanagishima
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Control ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals Composition of the λ plasmid heritable replicationcomplex

2002 ◽  
Vol 364 (3) ◽  
pp. 857-862 ◽  
Author(s):  
Katarzyna POTRYKUS ◽  
Sylwia BARAŃSKA ◽  
Alicja WĘGRZYN ◽  
Grzegorz WĘGRZYN
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Protein Synthesis ◽  
Plasmid Dna ◽  
Pcr Analysis ◽  
Cross Linking ◽  
Bacteriophage Λ ◽  
Yeast Saccharomyces Cerevisiae ◽  
Replication Complex

Previous studies indicated during replication of plasmids derived from bacteriophage λ (the so-called λ plasmids), that, once assembled, replication complex can be inherited by one of the two daughter plasmid copies after each replication round, and may function in subsequent replication rounds. It seems that similar processes occur during replication of other DNA molecules, including chromosomes of the yeast Saccharomyces cerevisiae. However, apart from some suggestions based on genetic experiments, composition of the λ heritable replication complex remains unknown. In amino acid-starved Escherichia coli relA mutants, replication of λ plasmid DNA is carried out exclusively by the heritable replication complex as assembly of new complexes is impaired due to inhibition of protein synthesis. Here, using a procedure based on in vivo cross-linking, cell lysis, immunoprecipitation with specific sera, de-cross-linking and PCR analysis, we demonstrate that the λ heritable replication complex consists of O, P, DnaB and, perhaps surprisingly, DnaK proteins.

scholarly journals The genetic control of direct-repeat recombination in Saccharomyces: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionally regulated gene.

Genetics ◽  
1989 ◽  
Vol 123 (4) ◽  
pp. 725-738 ◽  
Author(s):  
B J Thomas ◽  
R Rothstein
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Control ◽  
Plasmid Dna ◽  
Double Mutant ◽  
Direct Repeat ◽  
Single Mutant ◽  
Plasmid Loss ◽  
Mutant Strains ◽  
Recombination Pathway

Abstract We have previously shown direct-repeat recombination events leading to loss of a plasmid integrated at the GAL10 locus in Saccharomyces cerevisiae are stimulated by transcription of the region. We have examined the role of two recombination- and repair-defective mutations, rad1 and rad52, on direct repeat recombination in transcriptionally active and inactive sequences. We show that the RAD52 gene is required for transcription-stimulated recombination events leading to loss of the integrated plasmid. Similarly, Gal+ events between the duplicated repeats that retain the integrated plasmid DNA (Gal+ Ura+ replacement events) are reduced 20-fold in the rad52 mutant in sequences that are constitutively expressed. In contrast, in sequences that are not expressed, the rad52 mutation reduces plasmid loss events by only twofold and Gal+ Ura+ replacements by fourfold. We also observe an increase in disome-associated plasmid loss events in the rad52 mutant, indicative of chromosome gain. This event is not affected by expression of the region. Plasmid loss events in rad1 mutant strains are reduced only twofold in transcriptionally active sequences and are not affected in sequences that are repressed. However, the rad1 and rad52 double mutant shows a decrease in plasmid loss events greater than the sum of the decreases in the rates of this event displayed by either single mutant in both constitutive and repressed DNA, indicating a synergistic interaction between these two genes. The synergism is limited to recombination since the rad1 rad52 double mutant is no more sensitive when compared with either single mutant in its ability to survive radiation damage. Finally, the recombination pathway that remains in the double mutant is positively affected by transcription of the region.

The use of plasmid DNA to probe DNA repair functions in the yeast Saccharomyces cerevisiae

1985 ◽  
Vol 201 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Charles I. White ◽  
Steven G. Sedgwick
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Plasmid Dna ◽  
Yeast Saccharomyces Cerevisiae

Genetic control of metabolism of mutagenic purine base analogs 6-hydroxylaminopurine and 2-amino-6-hydroxylaminopurine in yeast Saccharomyces cerevisiae

2009 ◽  
Vol 45 (4) ◽  
pp. 409-414 ◽  
Author(s):  
E. I. Stepchenkova ◽  
S. G. Kozmin ◽  
V. V. Alenin ◽  
Yu. I. Pavlov
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Control ◽  
Yeast Saccharomyces Cerevisiae ◽  
Purine Base

Repair of double-strand breaks in plasmid DNA in the yeast Saccharomyces cerevisiae

1988 ◽  
Vol 213 (2-3) ◽  
pp. 421-424 ◽  
Author(s):  
Joseph R. Perera ◽  
Alexander V. Glasunov ◽  
Vadim M. Glaser ◽  
Alla V. Boreiko
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasmid Dna ◽  
Double Strand Breaks ◽  
Yeast Saccharomyces Cerevisiae ◽  
Strand Breaks

Chromosomal assignment of mutations by specific chromosome loss in the yeast Saccharomyces cerevisiae.

Genetics ◽  
1990 ◽  
Vol 125 (2) ◽  
pp. 333-340 ◽  
Author(s):  
L P Wakem ◽  
F Sherman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasmid Dna ◽  
High Frequency ◽  
Recessive Mutation ◽  
Chromosomal Assignment ◽  
Chromosome Loss ◽  
Specific Chromosome ◽  
Yeast Saccharomyces Cerevisiae ◽  
Homologous Chromosomes

Abstract Yeast 2-microns plasmids were integrated near the centromere of a different chromosome in each of 16 cir0 mapping strains of Saccharomyces cerevisiae. The specific chromosomes containing the integrated 2-microns plasmid DNA were lost at a high frequency after crossing the cir0 strains to cir+ strains. A recessive mutation in a cir+ strain can then be easily assigned to its chromosome using this set of mapping strains, since the phenotype of the recessive mutation will be manifested only in diploids having the integrated 2-microns plasmid and the unmapped mutation on homologous chromosomes.

Genetic Control of the DNA Polymerase α-Primase complex in the Yeast Saccharomyces cerevisiae

1992 ◽  
pp. 285-294
Author(s):  
P. Plevani ◽  
M. Foiani ◽  
S. Francesconi ◽  
A. Pizzagalli ◽  
C. Santocanale ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Control ◽  
Dna Polymerase ◽  
Yeast Saccharomyces Cerevisiae ◽  
Dna Polymerase Α ◽  
Primase Complex
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