The use of plasmid DNA to probe DNA repair functions in the yeast Saccharomyces cerevisiae

1985 ◽  
Vol 201 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Charles I. White ◽  
Steven G. Sedgwick
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Plasmid Dna ◽  
Yeast Saccharomyces Cerevisiae

Genetic control of plasmid DNA double-strand gap repair in yeast, Saccharomyces cerevisiae

1990 ◽  
Vol 18 (1) ◽  
pp. 1-5 ◽  
Author(s):  
V. M. Glaser ◽  
A. V. Glasunov ◽  
G. G. Tevzadze ◽  
J. R. Perera ◽  
S. V. Shestakov
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Control ◽  
Plasmid Dna ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals The essential DNA polymerases delta and epsilon are involved in repair of UV-damaged DNA in the yeast Saccharomyces cerevisiae.

1999 ◽  
Vol 46 (2) ◽  
pp. 289-298 ◽  
Author(s):  
A Hałas ◽  
Z Policińska ◽  
H Baranowska ◽  
W J Jachymczyk
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Uv Irradiation ◽  
Dna Polymerases ◽  
Relative Molecular Mass ◽  
Yeast Saccharomyces Cerevisiae ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Mutant Strains ◽  
Cellular Dna

We have studied the ability of yeast DNA polymerases to carry out repair of lesions caused by UV irradiation in Saccharomyces cerevisiae. By the analysis of postirradiation relative molecular mass changes in cellular DNA of different DNA polymerases mutant strains, it was established that mutations in DNA polymerases delta and epsilon showed accumulation of single-strand breaks indicating defective repair. Mutations in other DNA polymerase genes exhibited no defects in DNA repair. Thus, the data obtained suggest that DNA polymerases delta and epsilon are both necessary for DNA replication and for repair of lesions caused by UV irradiation. The results are discussed in the light of current concepts concerning the specificity of DNA polymerases in DNA repair.

scholarly journals Composition of the λ plasmid heritable replicationcomplex

2002 ◽  
Vol 364 (3) ◽  
pp. 857-862 ◽  
Author(s):  
Katarzyna POTRYKUS ◽  
Sylwia BARAŃSKA ◽  
Alicja WĘGRZYN ◽  
Grzegorz WĘGRZYN
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Protein Synthesis ◽  
Plasmid Dna ◽  
Pcr Analysis ◽  
Cross Linking ◽  
Bacteriophage Λ ◽  
Yeast Saccharomyces Cerevisiae ◽  
Replication Complex

Previous studies indicated during replication of plasmids derived from bacteriophage λ (the so-called λ plasmids), that, once assembled, replication complex can be inherited by one of the two daughter plasmid copies after each replication round, and may function in subsequent replication rounds. It seems that similar processes occur during replication of other DNA molecules, including chromosomes of the yeast Saccharomyces cerevisiae. However, apart from some suggestions based on genetic experiments, composition of the λ heritable replication complex remains unknown. In amino acid-starved Escherichia coli relA mutants, replication of λ plasmid DNA is carried out exclusively by the heritable replication complex as assembly of new complexes is impaired due to inhibition of protein synthesis. Here, using a procedure based on in vivo cross-linking, cell lysis, immunoprecipitation with specific sera, de-cross-linking and PCR analysis, we demonstrate that the λ heritable replication complex consists of O, P, DnaB and, perhaps surprisingly, DnaK proteins.

Human XPA and XRCC1 DNA Repair Proteins Expressed in Yeast, Saccharomyces cerevisiae

2001 ◽  
Vol 74 (3) ◽  
pp. 380-384 ◽  
Author(s):  
Elena A. Pushnova ◽  
Kirill Ostanin ◽  
Michael P. Thelen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Yeast Saccharomyces Cerevisiae ◽  
Dna Repair Proteins

The role of PSO and SNM genes in DNA repair of the yeast Saccharomyces cerevisiae

1990 ◽  
Vol 18 (5) ◽  
pp. 387-393 ◽  
Author(s):  
Jo�o A. P. Henriques ◽  
Martin Brendel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Yeast Saccharomyces Cerevisiae

Pph3 Phosphatase Participates in the Regulation of the Error-Free Branch of Postreplication DNA Repair in Yeast Saccharomyces cerevisiae

2021 ◽  
Vol 57 (2) ◽  
pp. 152-160
Author(s):  
D. V. Fedorov ◽  
T. A. Evstyukhina ◽  
V. T. Peshekhonov ◽  
V. G. Korolev
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Yeast Saccharomyces Cerevisiae

Sensitivity to Sn2+ of the Yeast Saccharomyces cerevisiae Depends on General Energy Metabolism, Metal Transport, Anti-Oxidative Defences, and DNA Repair

BioMetals ◽  
2006 ◽  
Vol 19 (6) ◽  
pp. 705-714 ◽  
Author(s):  
C. Viau ◽  
C. Pungartnik ◽  
M.C. Schmitt ◽  
T.S. Basso ◽  
J.A.P. Henriques ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Energy Metabolism ◽  
Metal Transport ◽  
Yeast Saccharomyces Cerevisiae ◽  
General Energy

Repair of double-strand breaks in plasmid DNA in the yeast Saccharomyces cerevisiae

1988 ◽  
Vol 213 (2-3) ◽  
pp. 421-424 ◽  
Author(s):  
Joseph R. Perera ◽  
Alexander V. Glasunov ◽  
Vadim M. Glaser ◽  
Alla V. Boreiko
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasmid Dna ◽  
Double Strand Breaks ◽  
Yeast Saccharomyces Cerevisiae ◽  
Strand Breaks

Chromosomal assignment of mutations by specific chromosome loss in the yeast Saccharomyces cerevisiae.

Genetics ◽  
1990 ◽  
Vol 125 (2) ◽  
pp. 333-340 ◽  
Author(s):  
L P Wakem ◽  
F Sherman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasmid Dna ◽  
High Frequency ◽  
Recessive Mutation ◽  
Chromosomal Assignment ◽  
Chromosome Loss ◽  
Specific Chromosome ◽  
Yeast Saccharomyces Cerevisiae ◽  
Homologous Chromosomes

Abstract Yeast 2-microns plasmids were integrated near the centromere of a different chromosome in each of 16 cir0 mapping strains of Saccharomyces cerevisiae. The specific chromosomes containing the integrated 2-microns plasmid DNA were lost at a high frequency after crossing the cir0 strains to cir+ strains. A recessive mutation in a cir+ strain can then be easily assigned to its chromosome using this set of mapping strains, since the phenotype of the recessive mutation will be manifested only in diploids having the integrated 2-microns plasmid and the unmapped mutation on homologous chromosomes.

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