Growth rate-dependent regulation of RNA polymerase synthesis in Escherichia coli

1985 ◽  
Vol 201 (3) ◽  
pp. 379-386 ◽  
Author(s):  
Geoffrey Ralling ◽  
Sharon Bodrug ◽  
Thomas Linn
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Rna Polymerase ◽  
Rate Dependent

scholarly journals DksA Is Required for Growth Phase-Dependent Regulation, Growth Rate-Dependent Control, and Stringent Control of fis Expression in Escherichia coli

2006 ◽  
Vol 188 (16) ◽  
pp. 5775-5782 ◽  
Author(s):  
Prabhat Mallik ◽  
Brian J. Paul ◽  
Steven T. Rutherford ◽  
Richard L. Gourse ◽  
Robert Osuna
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Amino Acid ◽  
Rna Polymerase ◽  
Growth Phase ◽  
Stationary Growth ◽  
Rate Dependent ◽  
Transcription In Vitro ◽  
State Growth

ABSTRACT DksA is a critical transcription factor in Escherichia coli that binds to RNA polymerase and potentiates control of rRNA promoters and certain amino acid promoters. Given the kinetic similarities between rRNA promoters and the fis promoter (Pfis), we investigated the possibility that DksA might also control transcription from Pfis. We show that the absence of dksA extends transcription from Pfis well into the late logarithmic and stationary growth phases, demonstrating the importance of DksA for growth phase-dependent regulation of fis. We also show that transcription from Pfis increases with steady-state growth rate and that dksA is absolutely required for this regulation. In addition, both DksA and ppGpp are required for inhibition of Pfis promoter activity following amino acid starvation, and these factors act directly and synergistically to negatively control Pfis transcription in vitro. DksA decreases the half-life of the intrinsically short-lived fis promoter-RNA polymerase complex and increases its sensitivity to the concentration of CTP, the predominant initiating nucleotide triphosphate for this promoter. This work extends our understanding of the multiple factors controlling fis expression and demonstrates the generality of the DksA requirement for regulation of kinetically similar promoters.

Growth-rate dependent regulation of mRNA stability in Escherichia coli

Nature ◽  
1984 ◽  
Vol 312 (5989) ◽  
pp. 75-77 ◽  
Author(s):  
G. Nilsson ◽  
J. G. Belasco ◽  
S. N. Cohen ◽  
A. von Gabain
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Mrna Stability ◽  
Rate Dependent

scholarly journals The Feedback Response of Escherichia coli rRNA Synthesis Is Not Identical to the Mechanism of Growth Rate-Dependent Control

2000 ◽  
Vol 182 (2) ◽  
pp. 536-539 ◽  
Author(s):  
Justina Voulgaris ◽  
Dmitry Pokholok ◽  
W. Mike Holmes ◽  
Craig Squires ◽  
Catherine L. Squires
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Gene Dosage ◽  
Rrna Synthesis ◽  
Rate Dependent ◽  
Nucleoside Triphosphates ◽  
The Relationship ◽  
Mechanism Of Growth

ABSTRACT Growth rate-independent rrn P1 promoter mutants were tested for their ability to respond to changes in rrn gene dosage. Most were found to be normal for the feedback response. In addition, cellular levels of the initiating nucleoside triphosphates remained unchanged when the rrn gene dosage was altered. These results suggest that the feedback response cannot be the mechanism for growth rate-dependent control of rRNA synthesis and that the relationship between these two processes may be more complicated than is currently understood.

scholarly journals The UP Element Is Necessary but Not Sufficient for Growth Rate-Dependent Control of the Escherichia coli guaB Promoter

2008 ◽  
Vol 190 (7) ◽  
pp. 2450-2457 ◽  
Author(s):  
Seyyed I. Husnain ◽  
Mark S. Thomas
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Transcription Start Site ◽  
De Novo ◽  
Start Site ◽  
Transcription Start ◽  
Lower Growth ◽  
Α Subunit ◽  
Rate Dependent ◽  
Up Elements

ABSTRACT The Escherichia coli guaB promoter (P guaB ) regulates the transcription of two genes, guaB and guaA, that are required for de novo synthesis of GMP, a precursor for the synthesis of guanine nucleoside triphosphates. The activity of P guaB is subject to growth rate-dependent control (GRDC). Here we show that the A+T-rich sequence located between positions −59 and −38 relative to the guaB transcription start site stimulates transcription from P guaB ∼8- to 10-fold and, in common with other UP elements, requires the C-terminal domain of the RNA polymerase α subunit for activity. Like the rrnB P1 UP element, the P guaB UP element contains two independently acting subsites located at positions −59 to −47 and −46 to −38 and can stimulate transcription when placed upstream of the lacP1 promoter. We reveal a novel role for the P guaB UP element by demonstrating that it is required for GRDC. The involvement of the UP element in GRDC also requires the participation of sequences located at least 100 bp upstream of the guaB transcription start site. These sequences are required for down-regulation of P guaB activity at lower growth rates.

scholarly journals Host factor I, Hfq, binds to Escherichia coli ompA mRNA in a growth rate-dependent fashion and regulates its stability

1998 ◽  
Vol 95 (24) ◽  
pp. 14118-14123 ◽  
Author(s):  
O. Vytvytska ◽  
J. S. Jakobsen ◽  
G. Balcunaite ◽  
J. S. Andersen ◽  
M. Baccarini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Host Factor ◽  
Factor I ◽  
Rate Dependent
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