Determination of cell membrane resistance in cultured renal epithelioid (MDCK) cells: effects of cadmium and mercury ions

1990 ◽  
Vol 417 (1) ◽  
pp. 29-36 ◽  
Author(s):  
M. Ritter ◽  
F. Lang ◽  
G. Gr�bl ◽  
H. G. Embacher
Keyword(s):  
Cell Membrane ◽  
Mdck Cells ◽  
Membrane Resistance ◽  
Mercury Ions ◽  
Cell Membrane Resistance

Electrical Resistance and Volume Flow in Glass Microelectrodes

1971 ◽  
Vol 49 (5) ◽  
pp. 436-447 ◽  
Author(s):  
D. R. Firth ◽  
L. J. DeFelice
Keyword(s):  
Cell Membrane ◽  
Electrical Resistance ◽  
Surface Condition ◽  
External Solution ◽  
Membrane Resistance ◽  
Volume Flow ◽  
Glass Wall ◽  
Measuring Cell ◽  
Cell Membrane Resistance ◽  
Flow Effects

The electrical resistance and the flow of solution in glass microelectrodes has been studied as function of the concentration of internal and external solution (KCl, 3–10−5 M), and as a function of pressure between the inside and outside solutions. The study demonstrates the usefulness of using microelectrodes for measuring cell membrane resistance and also the dangers in using them as microejectors because of anomalous flow effects. Electrokinetic effects and tip potentials are briefly described; they offer a means of investigating the surface condition of the glass wall inside the tip of the electrode and its variation with the outside solution.

scholarly journals Higher Plant Cell Membrane Resistance by a Single Intracellular Electrode Method

1974 ◽  
Vol 53 (1) ◽  
pp. 122-124 ◽  
Author(s):  
W. P. Anderson ◽  
D. L. Hendrix ◽  
N. Higinbotham
Keyword(s):  
Cell Membrane ◽  
Plant Cell ◽  
Membrane Resistance ◽  
Higher Plant ◽  
Cell Membrane Resistance ◽  
Electrode Method

Progesterone inhibits K conductance in plasma membrane of cultured renal epitheloid MDCK cells

1991 ◽  
Vol 260 (5) ◽  
pp. E743-E750 ◽  
Author(s):  
M. Steidl ◽  
G. Pinggera ◽  
M. Ritter ◽  
F. Lang
Keyword(s):  
Cell Membrane ◽  
Channel Blocker ◽  
Mdck Cells ◽  
Extracellular Fluid ◽  
Potassium Conductance ◽  
Membrane Resistance ◽  
Acute Administration ◽  
Madin Darby Canine Kidney ◽  
Ru 486 ◽  
Darby Canine Kidney

Progesterone causes natriuresis, an effect largely attributed to displacement of aldosterone from its receptor. The present study, however, demonstrates that progesterone (0.1, 1, and 10 mumol/1, respectively) also causes a rapid, fully reversible depolarization of Madin-Darby canine kidney (MDCK) cells (by 1.3 +/- 0.5, 4.1 +/- 0.7 and 12.3 +/- 1.5 mV, respectively). 17 alpha-Hydroxyprogesterone and dihydroxytestosterone are, by two orders of magnitude, less effective, whereas cholesterol, aldosterone, hydrocortisone, and estradiol (each up to 10 mumol/l) did not significantly alter the potential difference across the cell membrane. The effect of progesterone is blunted by antiprogestogen RU 486 (5 mumol/l). The progesterone-induced depolarization is paralleled by a decrease of potassium selectivity and an increase of cell membrane resistance and is abolished in the presence of the potassium channel blocker barium (10 mmol/l), as well as in the presence of 40 mmol/l potassium in the extracellular fluid. Neither removal of extracellular chloride or bicarbonate nor amiloride, ouabain, or pretreatment with pertussis toxin abolish the depolarizing effect of 5 mumol/l progesterone. In conclusion, acute administration of progesterone depolarizes MDCK cells by decreasing the potassium conductance of the cell membrane.

Luminal acid increases apical cell membrane resistance in isolated Necturus antral mucosa

1997 ◽  
Vol 113 (3) ◽  
pp. 875-883 ◽  
Author(s):  
H Mustonen ◽  
E Kivilaakso
Keyword(s):  
Cell Membrane ◽  
Apical Cell ◽  
Membrane Resistance ◽  
Antral Mucosa ◽  
Apical Cell Membrane ◽  
Cell Membrane Resistance

Differences in resistance against osmotic challenge among C57BL/6, DBA/2 and their hybrid mice metaphase II (MII) stage oocytes

Zygote ◽  
2019 ◽  
Vol 27 (4) ◽  
pp. 250-254
Author(s):  
Mei Goto ◽  
Hirokatsu Saito ◽  
Yuki Hiradate ◽  
Kenshiro Hara ◽  
Kentaro Tanemura
Keyword(s):  
Cell Membrane ◽  
Osmotic Pressure ◽  
Intracytoplasmic Sperm Injection ◽  
Inbred Mice ◽  
Membrane Resistance ◽  
Hybrid Vigour ◽  
High Tolerance ◽  
Cell Membrane Resistance ◽  
Osmotic Challenge ◽  
Hybrid Mice

SummaryOocytes of B6D2F1 (BDF1) mice are often used as recipients for intracytoplasmic sperm injection because of their cell membrane resistance against capillary penetration. It is assumed that oocytes of BDF1 mice have superior traits because of their hybrid vigour. However, the mechanisms of hybrid vigour are unclear. In this study, we focused on the membrane resistance of MII stage oocytes against changes in extracellular osmotic pressure. As a result, MII stage oocytes of inbred C57BL/6 and DBA/2 mice showed high tolerance in either a hypertonic or a hypotonic environment. Conversely, MII stage oocytes of hybrid BDF1 and D2B6F1 mice showed high tolerance in both hypertonic and hypotonic environments. Therefore, it is considered that MII stage oocytes of hybrid mice have superior traits than those of inbred mice. Our findings demonstrated that the hybrid vigour exists in the form of resistance to extracellular osmotic environment in hybrid MII stage oocytes.

Insulin decreases apical cell membrane resistance in cultured kidney cells (A6)

1984 ◽  
Vol 124 (2) ◽  
pp. 614-618 ◽  
Author(s):  
T.C. Walker ◽  
M.L. Fidelman ◽  
C.O. Watlington ◽  
T.U.L. Biber
Keyword(s):  
Cell Membrane ◽  
Apical Cell ◽  
Membrane Resistance ◽  
Kidney Cells ◽  
Apical Cell Membrane ◽  
Cell Membrane Resistance

The effect of chlorpromazine on cell membrane resistance and capacitance

1977 ◽  
Vol 45 (3) ◽  
pp. 251-256 ◽  
Author(s):  
Peter G. Smith
Keyword(s):  
Cell Membrane ◽  
Membrane Resistance ◽  
Cell Membrane Resistance

Effects of Osmotic Stress, Ionic Stress and IAA on the Cell-Membrane Resistance of Vigna Hypocotyls

1991 ◽  
Vol 32 (7) ◽  
pp. 1021-1029 ◽  
Author(s):  
Qiang Liu ◽  
Kiyoshi Katou ◽  
Hisashi Okamoto
Keyword(s):  
Cell Membrane ◽  
Osmotic Stress ◽  
Membrane Resistance ◽  
Cell Membrane Resistance ◽  
Ionic Stress

ALTERATIONS IN GLIAL AND MESENCHYMAL TUMOR CELL MEMBRANE RESISTANCE WITH HETEROIMMUNE AND AUTOLOGOUS SERA

1969 ◽  
Vol 159 (2 Research in t) ◽  
pp. 585-590 ◽  
Author(s):  
Paul L. Kornblith ◽  
Albert Prieto ◽  
Daniel A. Pollen
Keyword(s):  
Cell Membrane ◽  
Tumor Cell ◽  
Membrane Resistance ◽  
Mesenchymal Tumor ◽  
Tumor Cell Membrane ◽  
Cell Membrane Resistance
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