Electrical Resistance and Volume Flow in Glass Microelectrodes

1971 ◽  
Vol 49 (5) ◽  
pp. 436-447 ◽  
Author(s):  
D. R. Firth ◽  
L. J. DeFelice
Keyword(s):  
Cell Membrane ◽  
Electrical Resistance ◽  
Surface Condition ◽  
External Solution ◽  
Membrane Resistance ◽  
Volume Flow ◽  
Glass Wall ◽  
Measuring Cell ◽  
Cell Membrane Resistance ◽  
Flow Effects

The electrical resistance and the flow of solution in glass microelectrodes has been studied as function of the concentration of internal and external solution (KCl, 3–10−5 M), and as a function of pressure between the inside and outside solutions. The study demonstrates the usefulness of using microelectrodes for measuring cell membrane resistance and also the dangers in using them as microejectors because of anomalous flow effects. Electrokinetic effects and tip potentials are briefly described; they offer a means of investigating the surface condition of the glass wall inside the tip of the electrode and its variation with the outside solution.

scholarly journals Higher Plant Cell Membrane Resistance by a Single Intracellular Electrode Method

1974 ◽  
Vol 53 (1) ◽  
pp. 122-124 ◽  
Author(s):  
W. P. Anderson ◽  
D. L. Hendrix ◽  
N. Higinbotham
Keyword(s):  
Cell Membrane ◽  
Plant Cell ◽  
Membrane Resistance ◽  
Higher Plant ◽  
Cell Membrane Resistance ◽  
Electrode Method

Differences in resistance against osmotic challenge among C57BL/6, DBA/2 and their hybrid mice metaphase II (MII) stage oocytes

Zygote ◽  
2019 ◽  
Vol 27 (4) ◽  
pp. 250-254
Author(s):  
Mei Goto ◽  
Hirokatsu Saito ◽  
Yuki Hiradate ◽  
Kenshiro Hara ◽  
Kentaro Tanemura
Keyword(s):  
Cell Membrane ◽  
Osmotic Pressure ◽  
Intracytoplasmic Sperm Injection ◽  
Inbred Mice ◽  
Membrane Resistance ◽  
Hybrid Vigour ◽  
High Tolerance ◽  
Cell Membrane Resistance ◽  
Osmotic Challenge ◽  
Hybrid Mice

SummaryOocytes of B6D2F1 (BDF1) mice are often used as recipients for intracytoplasmic sperm injection because of their cell membrane resistance against capillary penetration. It is assumed that oocytes of BDF1 mice have superior traits because of their hybrid vigour. However, the mechanisms of hybrid vigour are unclear. In this study, we focused on the membrane resistance of MII stage oocytes against changes in extracellular osmotic pressure. As a result, MII stage oocytes of inbred C57BL/6 and DBA/2 mice showed high tolerance in either a hypertonic or a hypotonic environment. Conversely, MII stage oocytes of hybrid BDF1 and D2B6F1 mice showed high tolerance in both hypertonic and hypotonic environments. Therefore, it is considered that MII stage oocytes of hybrid mice have superior traits than those of inbred mice. Our findings demonstrated that the hybrid vigour exists in the form of resistance to extracellular osmotic environment in hybrid MII stage oocytes.

scholarly journals Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells

2020 ◽  
Vol 11 (3) ◽  
pp. 702-715 ◽  
Author(s):  
Michael Gorry ◽  
Toshie Yoneyama ◽  
Lazar Vujanovic ◽  
Marcia L. Moss ◽  
Michelle A. Garlin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Enzyme Activity ◽  
Cell Membrane ◽  
Measuring Cell ◽  
Membrane Enzyme

scholarly journals Resistivity behavior of leather after electro-conductive treatment

2019 ◽  
Vol 2 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Aulon Shabani ◽  
Majlinda Hylli ◽  
Ilda Kazani ◽  
Pellumb Berberi
Keyword(s):  
Electrical Resistivity ◽  
Environmental Conditions ◽  
Electrical Resistance ◽  
Chemical Processes ◽  
Bulk Conductivity ◽  
Measuring Cell ◽  
Textile Materials ◽  
Multiple Factors ◽  
Conductive Properties ◽  
First Time

Measurement of electrical resistance of textile materials, fiber and fabrics included, remains always an engaging task due to sensitivities to interference of multiple factors. Difficulty stands on both finding a method of measurements that fits the requirements of samples to be tested and the most appropriate indicator describing this property. Numerous methods and indicators are used for different sample content and shape (fibers, roving, yarn or fabric, etc.), even when the material tested is the same. Different methods usually use indicators that produce results difficult to compare or to interpret, or do not express intrinsic qualities of their constituent materials. The situation is the same for leather materials. In this paper, we propose a new method, multiple steps method, and a new indicator, electrical resistivity, which takes into consideration compressional properties of leather sample and produce results independent from the amount and form of the sample. Electrical resistivity of conductive leather, as defined below, is shown to be an inherent indicator of bulk conductivity of leather assembly and is not influenced by sample form or the way it is placed within the measuring cell. The method is used for the first time to evaluate changes in electrical resistivity of leather after various chemical processes to make it electro-conductive. The data provide important information about the evolution of electro-conductive properties of leather at different stages of processing, as well as the influence of environmental conditions.

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