Quantitation of gibberellins and the metabolism of [3H]gibberellin A1 during somatic embryogenesis in carrot and anise cell cultures

Planta ◽  
1982 ◽  
Vol 155 (5) ◽  
pp. 369-376 ◽  
Author(s):  
Masana Noma ◽  
Jochen Huber ◽  
Dieter Ernst ◽  
Richard P. Pharis
Keyword(s):  
Somatic Embryogenesis ◽  
Cell Cultures ◽  
Gibberellin A1

Initiation and morphological development of somatic embryoids from barley cell cultures

1984 ◽  
Vol 62 (6) ◽  
pp. 1245-1249 ◽  
Author(s):  
L. S. Kott ◽  
K. J. Kasha
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Cell Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Morphological Development ◽  
Low Concentrations ◽  
The Media ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Barley Cell

Somatic embryogenesis was induced in callus previously initiated from immature embryos of barley. These cultures ranged in age from 6 weeks to 30 months. Embryoids were readily initiated from homogenized suspension-grown aggregates when plated on modified B5 media with 2,4-dichlorophenoxyacetic acid. Low concentrations (0.1 and 0.05 mg∙L−1) of abscisic acid promoted further maturation of embryoids, while gibberellic acid (1 mg∙L−1) and kinetin (0.1 mg∙L−1) were used in the media to encourage embryoid germination. The development of somatic embryoids from initiation through maturation and germination is described.

Characteristics of Citrus cell cultures during undifferentiated growth on sucrose and somatic embryogenesis on galactose

1995 ◽  
Vol 93 (3) ◽  
pp. 464-470
Author(s):  
Cecile Cabasson ◽  
Patrick Ollitrault ◽  
Francois-Xavier Cote ◽  
Nicole Michaux-Ferriere ◽  
Dominique Dambier ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Cell Cultures

scholarly journals Somatic Embryogenesis in Arabidopsis thaliana Is Facilitated by Mutations in Genes Repressing Meristematic Cell Divisions

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 549-563
Author(s):  
Andreas P Mordhorst ◽  
Keete J Voerman ◽  
Marijke V Hartog ◽  
Ellen A Meijer ◽  
Jacques van Went ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cell Cultures ◽  
Egg Cell ◽  
Zygotic Embryos ◽  
Additive Effects ◽  
Additional Advantage ◽  
Embryogenic Cell ◽  
Immature Zygotic Embryos

Abstract Embryogenesis in plants can commence from cells other than the fertilized egg cell. Embryogenesis initiated from somatic cells in vitro is an attractive system for studying early embryonic stages when they are accessible to experimental manipulation. Somatic embryogenesis in Arabidopsis offers the additional advantage that many zygotic embryo mutants can be studied under in vitro conditions. Two systems are available. The first employs immature zygotic embryos as starting material, yielding continuously growing embryogenic cultures in liquid medium. This is possible in at least 11 ecotypes. A second, more efficient and reproducible system, employing the primordia timing mutant (pt allelic to hpt, cop2, and amp1), was established. A significant advantage of the pt mutant is that intact seeds, germinated in 2,4-dichlorophenoxyacetic acid (2,4-D) containing liquid medium, give rise to stable embryonic cell cultures, circumventing tedious hand dissection of immature zygotic embryos. pt zygotic embryos are first distinguishable from wild type at early heart stage by a broader embryonic shoot apical meristem (SAM). In culture, embryogenic clusters originate from the enlarged SAMs. pt somatic embryos had all characteristic embryo pattern elements seen in zygotic embryos, but with higher and more variable numbers of cells. Embryogenic cell cultures were also established from seedling, of other mutants with enlarged SAMs, such as clavata (clv). pt clv double mutants showed additive effects on SAM size and an even higher frequency of seedlings producing embryogenic cell lines. pt clv double mutant plants had very short fasciated inflorescence stems and additive effects on the number of rosette leaves. This suggests that the PT and CLV genes act in independent pathways that control SAM size. An increased population of noncommitted SAM cells may be responsible for facilitated establishment of somatic embryogenesis in Arabidopsis.

Somatic embryogenesis in cell cultures of birch (Betula pendula Roth.)

1990 ◽  
Vol 23 (2) ◽  
pp. 101-105 ◽  
Author(s):  
Ulrika Kurt�n ◽  
Anna Maria Nuutila ◽  
Veli Kauppinen ◽  
Matti Rousi
Keyword(s):  
Somatic Embryogenesis ◽  
Cell Cultures ◽  
Betula Pendula ◽  
Betula Pendula Roth
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