Influence of a crown-gall tumor initiation enhancer on bacterial attachment to the host plant cell wall

Planta ◽  
1982 ◽  
Vol 156 (4) ◽  
pp. 364-368 ◽  
Author(s):  
A. -M. Bouckaert-Urban ◽  
G. Brouwers ◽  
L. Thoelen ◽  
J. C. Vendrig
Keyword(s):  
Cell Wall ◽  
Host Plant ◽  
Plant Cell ◽  
Crown Gall ◽  
Plant Cell Wall ◽  
Bacterial Attachment ◽  
Tumor Initiation ◽  
Crown Gall Tumor

scholarly journals Glycoside Hydrolase Genes Are Required for Virulence ofAgrobacterium tumefaciensonBryophyllum daigremontianaand Tomato

2019 ◽  
Vol 85 (15) ◽  
Author(s):  
Stephanie L. Mathews ◽  
Haylea Hannah ◽  
Hillary Samagaio ◽  
Camille Martin ◽  
Eleanor Rodriguez-Rassi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Host Cell ◽  
Plant Cell ◽  
Crown Gall ◽  
Plant Cell Wall ◽  
Tumor Formation ◽  
Glycoside Hydrolases ◽  
Plant Host ◽  
Content Type ◽  
Limited Digestion

ABSTRACTAgrobacterium tumefaciensis a rhizosphere bacterium that can infect wound sites on plants. The bacterium transfers a segment of DNA (T-DNA) from the Ti plasmid to the plant host cell via a type IV secretion system where the DNA becomes integrated into the host cell chromosomes. The expression of T-DNA in the plant results in tumor formation. Although the binding of the bacteria to plant surfaces has been studied previously, there is little work on possible interactions of the bacteria with the plant cell wall. Seven of the 48 genes encoding putative glycoside hydrolases (Atu2295,Atu2371,Atu3104,Atu3129,Atu4560,Atu4561, andAtu4665) in the genome ofA. tumefaciensC58 were found to play a role in virulence on tomato andBryophyllum daigremontiana. Two of these genes (pglAandpglB;Atu3129andAtu4560) encode enzymes capable of digesting polygalacturonic acid and, thus, may play a role in the digestion of pectin. One gene (arfA;Atu3104) encodes an arabinosylfuranosidase, which could remove arabinose from the ends of polysaccharide chains. Two genes (bglAandbglB;Atu2295andAtu4561) encode proteins with β-glycosidase activity and could digest a variety of plant cell wall oligosaccharides and polysaccharides. One gene (xynA;Atu2371) encodes a putative xylanase, which may play a role in the digestion of xylan. Another gene (melA;Atu4665) encodes a protein with α-galactosidase activity and may be involved in the breakdown of arabinogalactans. Limited digestion of the plant cell wall byA. tumefaciensmay be involved in tumor formation on tomato andB. daigremontiana.IMPORTANCEA. tumefaciensis used in the construction of genetically engineered plants, as it is able to transfer DNA to plant hosts. Knowledge of the mechanisms of DNA transfer and the genes required will aid in the understanding of this process. Manipulation of glycoside hydrolases may increase transformation and widen the host range of the bacterium.A. tumefaciensalso causes disease (crown gall tumors) on a variety of plants, including stone fruit trees, grapes, and grafted ornamentals such as roses. It is possible that compounds that inhibit glycoside hydrolases could be used to control crown gall disease caused byA. tumefaciens.

scholarly journals Tumor Induction by Agrobacterium Involves Attachment of the Bacterium to a Site on the Host Plant Cell Wall

1977 ◽  
Vol 59 (3) ◽  
pp. 388-390 ◽  
Author(s):  
Barbara B. Lippincott ◽  
Mariamne H. Whatley ◽  
James A. Lippincott
Keyword(s):  
Cell Wall ◽  
Host Plant ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Tumor Induction ◽  
A Site

scholarly journals Pectin and Xyloglucan Influence the Attachment of Salmonella enterica and Listeria monocytogenes to Bacterial Cellulose-Derived Plant Cell Wall Models

2015 ◽  
Vol 82 (2) ◽  
pp. 680-688 ◽  
Author(s):  
Michelle S. F. Tan ◽  
Sadequr Rahman ◽  
Gary A. Dykes
Keyword(s):  
Cell Wall ◽  
Listeria Monocytogenes ◽  
Plant Cell ◽  
Salmonella Enterica ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Bacterial Attachment ◽  
Plant Cell Walls ◽  
Content Type ◽  
Wall Models

ABSTRACTMinimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. These pathogens must attach to the produce in order to be transmitted. Cut surfaces of produce that expose cell walls are particularly vulnerable. Little is known about the roles that different structural components (cellulose, pectin, and xyloglucan) of plant cell walls play in the attachment of foodborne bacterial pathogens. Using bacterial cellulose-derived plant cell wall models, we showed that the presence of pectin alone or xyloglucan alone affected the attachment of threeSalmonella entericastrains (Salmonella entericasubsp.entericaserovar Enteritidis ATCC 13076,Salmonella entericasubsp.entericaserovar Typhimurium ATCC 14028, andSalmonella entericasubsp.indicaM4) andListeria monocytogenesATCC 7644. In addition, we showed that this effect was modulated in the presence of both polysaccharides. Assays using pairwise combinations ofS.Typhimurium ATCC 14028 andL. monocytogenesATCC 7644 showed that bacterial attachment to all plant cell wall models was dependent on the characteristics of the individual bacterial strains and was not directly proportional to the initial concentration of the bacterial inoculum. This work showed that bacterial attachment was not determined directly by the plant cell wall model or bacterial physicochemical properties. We suggest that attachment of theSalmonellastrains may be influenced by the effects of these polysaccharides on physical and structural properties of the plant cell wall model. Our findings improve the understanding of howSalmonella entericaandListeria monocytogenesattach to plant cell walls, which may facilitate the development of better ways to prevent the attachment of these pathogens to such surfaces.

scholarly journals Hfq, a RNA Chaperone, Contributes to Virulence by Regulating Plant Cell Wall–Degrading Enzyme Production, Type VI Secretion System Expression, Bacterial Competition, and Suppressing Host Defense Response in Pectobacterium carotovorum

2018 ◽  
Vol 31 (11) ◽  
pp. 1166-1178 ◽  
Author(s):  
Chunting Wang ◽  
Tianxin Pu ◽  
Wangying Lou ◽  
Yujie Wang ◽  
Zishu Gao ◽  
...  
Keyword(s):  
Cell Wall ◽  
Host Plant ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Secretion System ◽  
Pectobacterium Carotovorum ◽  
Type Vi Secretion System ◽  
Degrading Enzyme ◽  
Bacterial Competition ◽  
Type Vi Secretion

Hfq is a RNA chaperone and participates in a wide range of cellular processes and pathways. In this study, mutation of hfq gene from Pectobacterium carotovorum subsp. carotovorum PccS1 led to significantly reduced virulence and plant cell wall–degrading enzyme (PCWDE) activities. In addition, the mutant exhibited decreased biofilm formation and motility and greatly attenuated carbapenem production as well as secretion of hemolysin coregulated protein (Hcp) as compared with wild-type strain PccS1. Moreover, a higher level of callose deposition was induced in Nicotiana benthamiana leaves when infiltrated with the mutant. A total of 26 small (s)RNA deletion mutants were obtained among a predicted 27 sRNAs, and three mutants exhibited reduced virulence in the host plant. These results suggest that hfq plays a key role in Pectobacterium virulence by positively impacting PCWDE production, secretion of the type VI secretion system, bacterial competition, and suppression of host plant responses.

A participation of weak-bound with plant cell wall peroxidases in ring rot tolerance of wild Mexican species and cultural varieties of potato

2014 ◽  
pp. 103-108
Author(s):  
M.A. Zhivetiev ◽  
◽  
A.V. Papkina ◽  
I.A. Graskova ◽  
V.K. Voinikov ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Ring Rot ◽  
Mexican Species
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