The lithium-chloride-soluble cell-wall layers of Chlamydomonas reinhardii contain several immunologically related glycoproteins

Planta ◽  
1988 ◽  
Vol 173 (3) ◽  
pp. 373-384 ◽  
Author(s):  
J�rgen Voigt
Keyword(s):  
Cell Wall ◽  
Lithium Chloride ◽  
Chlamydomonas Reinhardii ◽  
Soluble Cell

Biosynthesis and Turnover of Cell Wall Glycoproteins during the Vegetative Cell Cycle of Chlamydomonas reinhardii

1986 ◽  
Vol 41 (9-10) ◽  
pp. 885-896 ◽  
Author(s):  
Jürgen Voigt
Keyword(s):  
Cell Cycle ◽  
Cell Wall ◽  
Vegetative Cell ◽  
Wall Material ◽  
Cell Wall Material ◽  
Cell Enlargement ◽  
Chlamydomonas Reinhardii ◽  
Soluble Cell ◽  
Time Period ◽  
Wall Components

Abstract Biosynthesis and turnover of the different cell wall components have been studied during the vegetative cell cycle of Chlamydomonas reinhardii by pulse-labelling with [3H]proline and [35S]methionine and by pulse-chase experiments. Two phases of biosynthesis of insoluble cell wall material could be distinguished: 1. de novo synthesis of the daughter cell walls during cytokinesis and 2. cell wall enlargement during cell growth. During the cell enlargement period, a turnover of the insoluble wall component was observed. The released fragments were found to be accumulated in the culture medium. The LiCl-soluble cell wall glycoproteins were found to be precursors of the insoluble cell wall layer. Biosynthesis of the LiCl-soluble cell wall glycoproteins was observed mainly during the time period between cytokinesis and the end of the following cell enlargement period. Labelling of all the cell wall components was found to be strongly reduced during the time period between the end of the growth phase and cytokinesis. During cytokinesis, labelling of the insoluble cell wall material preceded the incorporation of radioactive precursors into the LiCl-soluble wall fraction.

Cell wall glycoproteins from Chlamydomonas reinhardii, and their self-assembly

Planta ◽  
1978 ◽  
Vol 138 (1) ◽  
pp. 91-98 ◽  
Author(s):  
J. W. Catt ◽  
G. J. Hills ◽  
K. Roberts
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Chlamydomonas Reinhardii

scholarly journals Coccidioides immitis Vaccine: Potential of an Alkali-Soluble, Water-Soluble Cell Wall Antigen

1983 ◽  
Vol 39 (1) ◽  
pp. 473-475 ◽  
Author(s):  
Grace Lecara ◽  
Rebecca A. Cox ◽  
Russell B. Simpson
Keyword(s):  
Cell Wall ◽  
Water Soluble ◽  
Coccidioides Immitis ◽  
Soluble Cell ◽  
Vaccine Potential

An acidic water-soluble cell wall polysaccharide: a chemotaxonomic marker for Fusarium and Gibberella

2000 ◽  
Vol 104 (5) ◽  
pp. 603-610 ◽  
Author(s):  
O. Ahrazem ◽  
B. Gómez-Miranda ◽  
A. Prieto ◽  
I. Barasoaín ◽  
M. Bernabé ◽  
...  
Keyword(s):  
Cell Wall ◽  
Water Soluble ◽  
Cell Wall Polysaccharide ◽  
Acidic Water ◽  
Soluble Cell ◽  
Chemotaxonomic Marker

scholarly journals Macromolecules released into the culture medium during the vegetative cell cycle of the unicellular green alga Chlamydomonas reinhardii

1985 ◽  
Vol 226 (1) ◽  
pp. 259-268 ◽  
Author(s):  
J Voigt
Keyword(s):  
Cell Cycle ◽  
Cell Wall ◽  
Cell Growth ◽  
Mother Cell ◽  
Culture Medium ◽  
Cell Enlargement ◽  
Chlamydomonas Reinhardii ◽  
Time Period ◽  
Protein Components ◽  
Mother Cell Wall

The culture medium of growing Chlamydomonas reinhardii cells contains hydroxyproline-rich glycoproteins, which are mainly liberated during release of the zoospores from the mother-cell wall. Pulse-labelling studies with [3H]proline and [35S]methionine have been performed in order to detect the protein components released by synchronously growing cells at different stages of the cell cycle. When either [3H]proline or [35S]methionine were applied during the phase of cell growth, radioactive label appeared in the released macromolecules after a lag period of 40 min, whereas incorporation into the insoluble part of the cell wall was delayed only by 20 min. When applied at the end of the growth phase, e.g. 13 h after beginning of the illumination period, the radioactive amino acids were incorporated into the cell wall, but radioactive labelling of macromolecules released into the culture medium could not be detected before the zoospores were liberated from the mother-cell wall. Maximal incorporation of [3H]proline and [35S]methionine into the insoluble part of the cell wall was observed during cell division, but essentially no radioactively-labelled macromolecules were released into the culture medium during this time period. Analysis of the macromolecules, which were liberated during cell enlargement, by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis revealed distinct radioactive bands, which were differentially labelled with [3H]proline and [35S]methionine. Among the macromolecules released into the culture medium during cell growth, a component of an apparent Mr 35 000 was preferentially labelled with [3H]proline. This component was also detected after labelling with [35S]methionine, but components of an apparently higher Mr were more prominent after labelling with [35S]methionine. Macromolecules released during the cell-enlargement period of synchronously growing cultures in the presence of [3H]proline contained radioactively-labelled hydroxyproline in addition to proline. These results show that, during cell-wall growth, specific protein components are released into the culture medium and that at least one of these components contains large amounts of proline and hydroxyproline. At least some of these macromolecules seem to be constituents of the cell wall, because during pulse-chase experiments radioactively-labelled macromolecules appeared in the culture medium mainly during the time period when the specific radioactivity of the insoluble inner-cell-wall layer decreased.

Synthesis of cell wall glucan, chitin, and protein by regenerating protoplasts and mycelia of Trichoderma reesei

1990 ◽  
Vol 36 (3) ◽  
pp. 211-217 ◽  
Author(s):  
Robert Messner ◽  
Christian P. Kubicek
Keyword(s):  
Cell Wall ◽  
Trichoderma Reesei ◽  
Cell Walls ◽  
Cell Wall Protein ◽  
Protoplast Regeneration ◽  
Secretory Proteins ◽  
Soluble Cell ◽  
Cell Wall Biogenesis ◽  
The Individual ◽  
Specific Labelling

The synthesis of constituent polymers of the cell wall by either growing mycelia or regenerating protoplasts of Trichoderma reesei QM 9414 was investigated by following the incorporation of radioactive precursors of the individual polymers (i.e., N[14C] acetyl-glucosamine, [3H] and [14C]glucose, [14C]mannose, and [35S]methionine) into individual fractions. N-Acetyl-glucosamine and glucose were found to become specifically incorporated into cell wall chitin and glucan by both mycelia and regenerating protoplasts, indicating the activity of chitin and glucan synthases in both systems. Cell wall glucan from regenerating protoplasts, however, consisted only of α-glucan and specifically lacked β-glucan which is found in mycelial cell walls. Mannose became metabolized to glucose before its label appeared in the cell wall, and was thus unsuitable for specific labelling. [35S]Methionine was found in a small (< 21 kDa) polypeptide from the first alkali-soluble cell wall fraction, but also in cell wall bound secretory proteins, i. e., β-glucosidase. These results indicate that cell wall biogenesis in T. reesei, in contrast to a report by other authors, is similar to that of other filamentous fungi. Key words: Trichoderma reesei, protoplast regeneration, cell wall polymer, β-glucan, cell wall protein.

scholarly journals Soluble Cell Wall Polysaccharides Released from Pea Stems by Centrifugation

1981 ◽  
Vol 68 (3) ◽  
pp. 538-542 ◽  
Author(s):  
Maurice E. Terry ◽  
Bernard Rubinstein ◽  
Russell L. Jones
Keyword(s):  
Cell Wall ◽  
Cell Wall Polysaccharides ◽  
Soluble Cell

Extraction by lithium chloride of hydroxyproline-rich glycoproteins from intact cells of Chlamydomonas reinhardii

Planta ◽  
1985 ◽  
Vol 164 (3) ◽  
pp. 379-389 ◽  
Author(s):  
J. Voigt
Keyword(s):  
Lithium Chloride ◽  
Intact Cells ◽  
Chlamydomonas Reinhardii

CONTENTS OF SOLUBLE, CELL-WALL-BOUND AND EXUDED PHLOROTANNINS IN THE BROWN ALGA Fucus vesiculosus, WITH IMPLICATIONS ON THEIR ECOLOGICAL FUNCTIONS

2005 ◽  
Vol 31 (1) ◽  
pp. 195-212 ◽  
Author(s):  
RIITTA KOIVIKKO ◽  
JYRKI LOPONEN ◽  
TUIJA HONKANEN ◽  
VEIJO JORMALAINEN
Keyword(s):  
Cell Wall ◽  
Brown Alga ◽  
Fucus Vesiculosus ◽  
Ecological Functions ◽  
Soluble Cell

scholarly journals Structural differences between the alkali-extracted water-soluble cell wall polysaccharides from mycelial and yeast phases of the pathogenic dimorphic fungus Paracoccidioides brasiliensis

Glycobiology ◽  
2003 ◽  
Vol 13 (11) ◽  
pp. 743-747 ◽  
Author(s):  
O. Ahrazem ◽  
A. Prieto ◽  
G. San-Blas ◽  
J. A. Leal ◽  
J. Jimenez-Barbero ◽  
...  
Keyword(s):  
Cell Wall ◽  
Paracoccidioides Brasiliensis ◽  
Water Soluble ◽  
Cell Wall Polysaccharides ◽  
Dimorphic Fungus ◽  
Soluble Cell ◽  
Structural Differences
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