The zygote cell wall of Chlamydomonas reinhardii: a structural, chemical and immunological approach

Planta ◽  
1987 ◽  
Vol 170 (4) ◽  
pp. 433-445 ◽  
Author(s):  
C. Grief ◽  
M. A. O'Neill ◽  
P. J. Shaw
Keyword(s):  
Cell Wall ◽  
Chlamydomonas Reinhardii ◽  
Immunological Approach

Cell wall glycoproteins from Chlamydomonas reinhardii, and their self-assembly

Planta ◽  
1978 ◽  
Vol 138 (1) ◽  
pp. 91-98 ◽  
Author(s):  
J. W. Catt ◽  
G. J. Hills ◽  
K. Roberts
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Chlamydomonas Reinhardii

scholarly journals Macromolecules released into the culture medium during the vegetative cell cycle of the unicellular green alga Chlamydomonas reinhardii

1985 ◽  
Vol 226 (1) ◽  
pp. 259-268 ◽  
Author(s):  
J Voigt
Keyword(s):  
Cell Cycle ◽  
Cell Wall ◽  
Cell Growth ◽  
Mother Cell ◽  
Culture Medium ◽  
Cell Enlargement ◽  
Chlamydomonas Reinhardii ◽  
Time Period ◽  
Protein Components ◽  
Mother Cell Wall

The culture medium of growing Chlamydomonas reinhardii cells contains hydroxyproline-rich glycoproteins, which are mainly liberated during release of the zoospores from the mother-cell wall. Pulse-labelling studies with [3H]proline and [35S]methionine have been performed in order to detect the protein components released by synchronously growing cells at different stages of the cell cycle. When either [3H]proline or [35S]methionine were applied during the phase of cell growth, radioactive label appeared in the released macromolecules after a lag period of 40 min, whereas incorporation into the insoluble part of the cell wall was delayed only by 20 min. When applied at the end of the growth phase, e.g. 13 h after beginning of the illumination period, the radioactive amino acids were incorporated into the cell wall, but radioactive labelling of macromolecules released into the culture medium could not be detected before the zoospores were liberated from the mother-cell wall. Maximal incorporation of [3H]proline and [35S]methionine into the insoluble part of the cell wall was observed during cell division, but essentially no radioactively-labelled macromolecules were released into the culture medium during this time period. Analysis of the macromolecules, which were liberated during cell enlargement, by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis revealed distinct radioactive bands, which were differentially labelled with [3H]proline and [35S]methionine. Among the macromolecules released into the culture medium during cell growth, a component of an apparent Mr 35 000 was preferentially labelled with [3H]proline. This component was also detected after labelling with [35S]methionine, but components of an apparently higher Mr were more prominent after labelling with [35S]methionine. Macromolecules released during the cell-enlargement period of synchronously growing cultures in the presence of [3H]proline contained radioactively-labelled hydroxyproline in addition to proline. These results show that, during cell-wall growth, specific protein components are released into the culture medium and that at least one of these components contains large amounts of proline and hydroxyproline. At least some of these macromolecules seem to be constituents of the cell wall, because during pulse-chase experiments radioactively-labelled macromolecules appeared in the culture medium mainly during the time period when the specific radioactivity of the insoluble inner-cell-wall layer decreased.

Methylation analysis of cell wall glycoproteins and glycopeptides from Chlamydomonas reinhardii

1981 ◽  
Vol 20 (1) ◽  
pp. 25-28 ◽  
Author(s):  
Malcolm A. O'neill ◽  
Keith Roberts
Keyword(s):  
Cell Wall ◽  
Methylation Analysis ◽  
Chlamydomonas Reinhardii

Synchronized cultures of a cell wall-less mutant of Chlamydomonas reinhardii

1976 ◽  
Vol 108 (2) ◽  
pp. 189-194 ◽  
Author(s):  
Torleiv Lien ◽  
Gjert Knutsen
Keyword(s):  
Cell Wall ◽  
Chlamydomonas Reinhardii ◽  
A Cell ◽  
Synchronized Cultures

Cell wall glycoproteins from Chlamydomonas reinhardii are sulphated

1980 ◽  
Vol 49 (4) ◽  
pp. 421-424 ◽  
Author(s):  
K. ROBERTS ◽  
M. R. GAY ◽  
G. J. HILLS
Keyword(s):  
Cell Wall ◽  
Chlamydomonas Reinhardii

Biosynthesis and Turnover of Cell Wall Glycoproteins during the Vegetative Cell Cycle of Chlamydomonas reinhardii

1986 ◽  
Vol 41 (9-10) ◽  
pp. 885-896 ◽  
Author(s):  
Jürgen Voigt
Keyword(s):  
Cell Cycle ◽  
Cell Wall ◽  
Vegetative Cell ◽  
Wall Material ◽  
Cell Wall Material ◽  
Cell Enlargement ◽  
Chlamydomonas Reinhardii ◽  
Soluble Cell ◽  
Time Period ◽  
Wall Components

Abstract Biosynthesis and turnover of the different cell wall components have been studied during the vegetative cell cycle of Chlamydomonas reinhardii by pulse-labelling with [3H]proline and [35S]methionine and by pulse-chase experiments. Two phases of biosynthesis of insoluble cell wall material could be distinguished: 1. de novo synthesis of the daughter cell walls during cytokinesis and 2. cell wall enlargement during cell growth. During the cell enlargement period, a turnover of the insoluble wall component was observed. The released fragments were found to be accumulated in the culture medium. The LiCl-soluble cell wall glycoproteins were found to be precursors of the insoluble cell wall layer. Biosynthesis of the LiCl-soluble cell wall glycoproteins was observed mainly during the time period between cytokinesis and the end of the following cell enlargement period. Labelling of all the cell wall components was found to be strongly reduced during the time period between the end of the growth phase and cytokinesis. During cytokinesis, labelling of the insoluble cell wall material preceded the incorporation of radioactive precursors into the LiCl-soluble wall fraction.

Assembly of cell-wall glycoproteins of Chlamydomonas reinhardii: Oligosaccharides are added in medial and trans Golgi compartments

Planta ◽  
1987 ◽  
Vol 171 (3) ◽  
pp. 302-312 ◽  
Author(s):  
C. Grief ◽  
P. J. Shaw
Keyword(s):  
Cell Wall ◽  
Chlamydomonas Reinhardii

Uptake of Azotobacters by Somatic Fusion of Cell-wall Mutants of Chlamydomonas reinhardii

1986 ◽  
Vol 181 (5) ◽  
pp. 347-357 ◽  
Author(s):  
N.H. Nghia ◽  
I. Gyurján ◽  
P. Stefanovits ◽  
GY. Paless ◽  
I. Turtóczky
Keyword(s):  
Cell Wall ◽  
Chlamydomonas Reinhardii ◽  
Somatic Fusion
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