The effect of strychnine sulphate on the home cage activity and oxygen consumption in three inbred strains of mice

Outbred LACA mice and inbred NIH mice were administered low (100 ova), medium (1000 ova), high (3000 ova) and trickle (4×250 ova) doses ofToxocara canisova and the effect of infection on activity was examined with respect to: (i) the dose of ova administered and (ii) the number of larvae recovered from the brain. Larval recovery from the brain was significantly reduced in NIH mice compared to LACA mice for the 1000, 3000 and trickle doses. Mice from each strain were divided into larval intensity groupings based upon the number of larvae recovered from their brain. Activity for each mouse was measured pre- and post-infection by observing its behaviour in the home cage. Activity was assessed by monitoring six different independent categories of murine behaviour – ambulation, grooming, rearing, digging, climbing and immobility. Within each behavioural category, the duration of time spent at each behaviour per mouse within one thousandth of a second, the number of short bouts performed and the number of long bouts of behaviour performed were recorded over a 20 min period. Activity of LACA and NIH mice differed prior to infection. LACA mice spent more time immobile compared to NIH mice, which ambulated and climbed more. Variations in activity were also observed between groups of mice prior to infection. The effect of infection differed by strain, by dose and by larval intensity. Post-infection LACA mice became more immobile and ambulated less. NIH mice showed reduced immobility, but while ambulation decreased digging and climbing increased post-infection. Short bouts of activity remained unchanged among LACA mice post-infection but showed an increase for some behaviours in NIH mice.

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Home cage activity and behavioral performance in inbred and hybrid mice

Behavioural Brain Research ◽

10.1016/s0166-4328(02)00228-0 ◽

2002 ◽

Vol 136 (2) ◽

pp. 555-569 ◽

Cited By ~ 90

Author(s):

Xiangdong Tang ◽

Stuart M Orchard ◽

Larry D Sanford

Keyword(s):

Home Cage ◽

Behavioral Performance ◽

Cage Activity ◽

Home Cage Activity ◽

Hybrid Mice

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Loss of hippocampal neurogenesis, increased novelty-induced activity, decreased home cage activity, and impaired reversal learning one year after irradiation of the young mouse brain

Experimental Neurology ◽

10.1016/j.expneurol.2013.01.006 ◽

2013 ◽

Vol 247 ◽

pp. 402-409 ◽

Cited By ~ 43

Author(s):

Marie Kalm ◽

Niklas Karlsson ◽

Marie K.L. Nilsson ◽

Klas Blomgren

Keyword(s):

Mouse Brain ◽

Reversal Learning ◽

Home Cage ◽

Hippocampal Neurogenesis ◽

Young Mouse ◽

Cage Activity ◽

One Year ◽

Home Cage Activity

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STUDIES ON MOUSE LEUKEMIA

Journal of Experimental Medicine ◽

10.1084/jem.60.5.547 ◽

1934 ◽

Vol 60 (5) ◽

pp. 547-558 ◽

Cited By ~ 3

Author(s):

Joseph Victor ◽

James S. Potter

Keyword(s):

Oxygen Consumption ◽

Cell Lines ◽

Inbred Strains ◽

Leukemic Cells ◽

Lymphoid Tissues ◽

Genetic Constitution ◽

Anaerobic Glycolysis ◽

Lymphatic Leukemia ◽

Inbred Strains Of Mice ◽

Aerobic And Anaerobic

1. Two highly inbred strains of mice of different genetic constitution (Storrs-Little and C58) were used in a study of the influence of hosts on metabolism of cells of transmissible lymphatic leukemia. The experiments were carried out with leukemic cells of transmission Line I as well as with Line M-liver. About 50 per cent of the Storrs-Little mice were killed by each line of cells at this time, while 100 per cent of the C58 mice were killed. 2. The normal lymphoid tissues of the two strains of mice were significantly the same in regard to rates of oxygen consumption and both aerobic and anaerobic glycolysis. 3. Leukemic cells of Line I, growing in hosts of Strain Storrs-Little, gave significantly lower rates of aerobic and anaerobic glycolysis than when growing in hosts of Strain C58. Oxygen consumption was significantly higher. Leukemic cells of Line M-liver, growing in hosts of Strain Storrs-Little, gave significantly lower rates of aerobic and anaerobic glycolysis than when growing in Strain C58. Oxygen consumption was not significantly different. 4. After one to three passages through hosts of Strain Storrs-Little, the cell lines were returned to hosts of Strain C58, with immediate return to significantly the same metabolic rates originally given by each line in hosts of Strain C58. 5. These results lead to the more general conclusions that: (a) The genetic constitution of the host modifies the metabolism of the cell line. (b) The same host constitution may modify the metabolism of different cell lines in different ways. (c) Host constitution does not appear to modify the inherent constitution of the leukemic cells, but acts as a determining environmental factor on their metabolism.

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Genetic study of multigenic factors related to strain difference of temporal pattern in mouse home-cage activity

Neuroscience Research ◽

10.1016/j.neures.2011.07.716 ◽

2011 ◽

Vol 71 ◽

pp. e166

Author(s):

Tatsuhiko Goto ◽

Ayako Ishii ◽

Akinori Nishi ◽

Aki Takahashi ◽

Toshihiko Shiroishi ◽

...

Keyword(s):

Genetic Study ◽

Temporal Pattern ◽

Home Cage ◽

Strain Difference ◽

Cage Activity ◽

Home Cage Activity

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Home-cage activity in heterogeneous stock (HS) mice as a model of baseline activity

Genes Brain & Behavior ◽

10.1034/j.1601-183x.2002.10304.x ◽

2002 ◽

Vol 1 (3) ◽

pp. 166-173 ◽

Cited By ~ 14

Author(s):

J. Mill ◽

M. J. Galsworthy ◽

J. L. Paya-Cano ◽

F. Sluyter ◽

L. C. Schalkwyk ◽

...

Keyword(s):

Home Cage ◽

Heterogeneous Stock ◽

Cage Activity ◽

Baseline Activity ◽

Home Cage Activity

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Chronic Stimulation of Arcuate Kiss1 Neurons Decreases Bone Mass in Female Mice

Journal of the Endocrine Society ◽

10.1210/jendso/bvab048.471 ◽

2021 ◽

Vol 5 (Supplement_1) ◽

pp. A232-A232

Author(s):

Ruben Rodriguez ◽

Candice B Herber ◽

William C Krause ◽

Holly A Ingraham

Keyword(s):

Bone Mass ◽

Home Cage ◽

Designer Drugs ◽

Reproductive Capacity ◽

Estrogen Signaling ◽

Female Mice ◽

Cage Activity ◽

Increased Risk ◽

Chronic Activation ◽

Home Cage Activity

Abstract Loss of peripheral estrogen in postmenopausal women is often associated with decreased physical activity and loss of bone mass, leading to an increased risk of metabolic diseases, osteoporosis, and skeletal fragility. While it is well-established that loss of peripheral estrogen signaling results in bone loss, we previously found that eliminating central estrogen signaling paradoxically results in an unexpected massive increase in bone mass only in female mice. Specifically, deletion of estrogen receptor alpha (ERα) signaling in kisspeptin 1 (Kiss1) expressing neurons of the arcuate nucleus (ARCKiss1) increases bone mass at the expense of reproduction in female mice. Currently, the mechanisms and the neurocircuits that modulate these unexpected responses are unknown. Here, to begin addressing these questions, we asked if changing the neuronal output of ARCKiss1 neurons using chemogenetic manipulation of ARCKiss1 neurons might also alter bone mass and locomotion in female mice. To do this, we delivered stimulatory (AAV2-hM3Dq-mCherry) designer receptors exclusively activated by designer drugs (DREADDs) to the ARC of wild type and Kiss1-Cre+ (Kiss1-CrehM3q-DREADDs) female mice and asked if chronic activation of ARCKiss1 neurons might alter bone mass as analyzed by standard ex-vivo µCT imaging. Clozapine N-oxide (CNO) was delivered for 22 days (0.1 mg/mL). We also leveraged the ANY-Maze system to assess home cage activity over an extensive 96-hour period. Acute activation of ARCKiss1 tended to decrease home cage activity by nearly 40% in Kiss1-CrehM3q-DREADDs mice during the dark period compared to WT females. Interestingly, chronic activation of ARCKiss1 neurons significantly lowered trabecular bone volume by nearly 30%. Current studies are underway to ask if inhibiting ARCKiss1 neurons results in increased bone mass. Our findings collectively suggest that the neuronal activity of ARCKiss1 neurons is sufficient to shift energy allocation away from locomotion and bone-building to maximize reproductive capacity. We speculate that the widely used SERM in breast cancer treatment, Tamoxifen, might exert its bone sparing effect by silencing ARCKiss1 neurons.

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